SNF2β-BRG1 is essential for the viability of F9 murine embryonal carcinoma cells

Chiho Ichinose, Hiroshi Ichinose, Daniel Metzger, Pierre Chambon

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

The yeast and animal SNF-SWI and related multiprotein complexes are thought to play an important role in processes, such as transcription factor binding to regulatory elements, which require nucleosome remodeling in order to relieve the repressing effect of packaging DNA in chromatin. There are two mammalian homologs of the yeast SNF2-SWI2 subunit protein, SNF2α-brm and SNF2β-BRG1, and overexpression of either one of them has been shown to enhance transcriptional activation by glucocorticoid, estrogen, and retinoic acid (RA) receptors in transiently transfected cells. We have investigated here the function of SNF2β-BRG1 in the RA receptor-retinoid X receptor- mediated transduction of the retinoid signal in F9 embryonal carcinoma (EC) cells which differentiate into endodermal-like cells upon RA treatment. The two SNF2β-BRG1 alleles have been targeted by homologous recombination and subsequently disrupted by using a conditional Cre recombinase. We show that F9 EC cells inactivated on both SNF2β alleles are not viable and that heterozygous mutant cells are affected in proliferation but not in RA- induced differentiation. Thus, in F9 EC cells, SNF2β-BRG1 appears to play an essential role in basal processes involved in cell proliferation, in addition to its putative role in the activation of transcription mediated by nuclear receptors.

Original languageEnglish
Pages (from-to)5976-5986
Number of pages11
JournalMolecular and Cellular Biology
Volume17
Issue number10
Publication statusPublished - 01-10-1997

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Embryonal Carcinoma Stem Cells
Retinoic Acid Receptors
Tretinoin
Transcriptional Activation
Yeasts
Alleles
DNA Packaging
Retinoid X Receptors
Multiprotein Complexes
Nucleosomes
Homologous Recombination
Retinoids
Protein Subunits
Cytoplasmic and Nuclear Receptors
Glucocorticoids
Chromatin
Signal Transduction
Estrogens
Transcription Factors
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Ichinose, Chiho ; Ichinose, Hiroshi ; Metzger, Daniel ; Chambon, Pierre. / SNF2β-BRG1 is essential for the viability of F9 murine embryonal carcinoma cells. In: Molecular and Cellular Biology. 1997 ; Vol. 17, No. 10. pp. 5976-5986.
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Ichinose, C, Ichinose, H, Metzger, D & Chambon, P 1997, 'SNF2β-BRG1 is essential for the viability of F9 murine embryonal carcinoma cells', Molecular and Cellular Biology, vol. 17, no. 10, pp. 5976-5986.

SNF2β-BRG1 is essential for the viability of F9 murine embryonal carcinoma cells. / Ichinose, Chiho; Ichinose, Hiroshi; Metzger, Daniel; Chambon, Pierre.

In: Molecular and Cellular Biology, Vol. 17, No. 10, 01.10.1997, p. 5976-5986.

Research output: Contribution to journalArticle

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