TY - JOUR
T1 - SNX25 regulates proinflammatory cytokine expression via the NF-?B signal in macrophages
AU - Nishimura, Kazuya
AU - Tanaka, Tatsuhide
AU - Takemura, Shoko
AU - Tatsumi, Kouko
AU - Wanaka, Akio
N1 - Publisher Copyright:
© 2021 Nishimura et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2021/3
Y1 - 2021/3
N2 - Innate immunity is the first line of defense against bacterial infection and is initiated by macrophages. Sorting nexin 25 (SNX25) is an SNX family member and is reported to negatively regulate TGF-ß signaling by enhancing TGF receptor degradation. However, few studies have focused on the relationship between SNX25 and the immune system. We knocked down SNX25 expression in macrophages and examined inflammatory cytokine expression, a hallmark of innate immunity, after lipopolysaccharide stimulation. SNX25 knockdown increased proinflammatory cytokine expression in RAW 264.7 cells. In addition, SNX25 knockdown activated the NF-?B signal by promoting ubiquitination of I?Ba. These results suggest that SNX25 inhibits the NF-?B signal and thereby regulates proinflammatory cytokine expression in macrophages.
AB - Innate immunity is the first line of defense against bacterial infection and is initiated by macrophages. Sorting nexin 25 (SNX25) is an SNX family member and is reported to negatively regulate TGF-ß signaling by enhancing TGF receptor degradation. However, few studies have focused on the relationship between SNX25 and the immune system. We knocked down SNX25 expression in macrophages and examined inflammatory cytokine expression, a hallmark of innate immunity, after lipopolysaccharide stimulation. SNX25 knockdown increased proinflammatory cytokine expression in RAW 264.7 cells. In addition, SNX25 knockdown activated the NF-?B signal by promoting ubiquitination of I?Ba. These results suggest that SNX25 inhibits the NF-?B signal and thereby regulates proinflammatory cytokine expression in macrophages.
UR - https://www.scopus.com/pages/publications/85102324097
UR - https://www.scopus.com/pages/publications/85102324097#tab=citedBy
U2 - 10.1371/journal.pone.0247840
DO - 10.1371/journal.pone.0247840
M3 - Article
C2 - 33647065
AN - SCOPUS:85102324097
SN - 1932-6203
VL - 16
JO - PloS one
JF - PloS one
IS - 3 March
M1 - e0247840
ER -