SOX10-Nano-lantern reporter human iPS cells; A versatile tool for neural crest research

Tomoko Horikiri, Hiromi Ohi, Mitsuaki Shibata, Makoto Ikeya, Morio Ueno, Chie Sotozono, Shigeru Kinoshita, Takahiko Sato

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The neural crest is a source to produce multipotent neural crest stem cells that have a potential to differentiate into diverse cell types. The transcription factor SOX10 is expressed through early neural crest progenitors and stem cells in vertebrates. Here we report the generation of SOX10-Nano-lantern (NL) reporter human induced pluripotent stem cells (hiPS) by using CRISPR/Cas9 systems, that are beneficial to investigate the generation and maintenance of neural crest progenitor cells. SOX10-NL positive cells are produced transiently from hiPS cells by treatment with TGFβ inhibitor SB431542 and GSK3 inhibitor CHIR99021. We found that all SOX10-NL-positive cells expressed an early neural crest marker NGFR, however SOX10-NL-positive cells purified from differentiated hiPS cells progressively attenuate their NL-expression under proliferation. We therefore attempted to maintain SOX10-NL-positive cells with additional signaling on the plane and sphere culture conditions. These SOX10-NL cells provide us to investigate mass culture with neural crest cells for stem cell research.

Original languageEnglish
Article numbere0170342
JournalPloS one
Volume12
Issue number1
DOIs
Publication statusPublished - 01-2017

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Horikiri, T., Ohi, H., Shibata, M., Ikeya, M., Ueno, M., Sotozono, C., Kinoshita, S., & Sato, T. (2017). SOX10-Nano-lantern reporter human iPS cells; A versatile tool for neural crest research. PloS one, 12(1), [e0170342]. https://doi.org/10.1371/journal.pone.0170342