TY - JOUR
T1 - Sox9 regulates the luminal stem/progenitor cell properties of salivary glands
AU - Tanaka, Junichi
AU - Mabuchi, Yo
AU - Hata, K.
AU - Yasuhara, Rika
AU - Takamatsu, Koki
AU - Kujiraoka, Satoko
AU - Yukimori, A.
AU - Takakura, I.
AU - Sumimoto, Hidetoshi
AU - Fukada, Toshiyuki
AU - Azuma, Masayuki
AU - Akiyama, Haruhiko
AU - Nishimura, Riko
AU - Shimane, Toshikazu
AU - Mishima, K.
N1 - Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Exocrine glands share a common morphology consisting of ductal, acinar, and basal/myoepithelial cells, but their functions and mechanisms of homeostasis differ among tissues. Salivary glands are an example of exocrine glands, and they have been reported to contain multipotent stem cells that differentiate into other tissues. In this study, we purified the salivary gland stem/progenitor cells of adult mouse salivary glands using the cell surface marker CD133 by flow cytometry. CD133+ cells possessed stem cell capacity, and the transplantation of CD133+ cells into the submandibular gland reconstituted gland structures, including functional acinar. CD133+ cells were sparsely distributed in the intercalated and exocrine ducts and expressed Sox9 at higher levels than CD133– cells. Moreover, we demonstrated that Sox9 was required for the stem cell properties CD133+ cells, including colony and sphere formation. Thus, the Sox9-related signaling may control the regeneration salivary glands.
AB - Exocrine glands share a common morphology consisting of ductal, acinar, and basal/myoepithelial cells, but their functions and mechanisms of homeostasis differ among tissues. Salivary glands are an example of exocrine glands, and they have been reported to contain multipotent stem cells that differentiate into other tissues. In this study, we purified the salivary gland stem/progenitor cells of adult mouse salivary glands using the cell surface marker CD133 by flow cytometry. CD133+ cells possessed stem cell capacity, and the transplantation of CD133+ cells into the submandibular gland reconstituted gland structures, including functional acinar. CD133+ cells were sparsely distributed in the intercalated and exocrine ducts and expressed Sox9 at higher levels than CD133– cells. Moreover, we demonstrated that Sox9 was required for the stem cell properties CD133+ cells, including colony and sphere formation. Thus, the Sox9-related signaling may control the regeneration salivary glands.
KW - CD133
KW - Salivary gland
KW - Sox9
KW - Stem/progenitor cells
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U2 - 10.1016/j.yexcr.2019.05.030
DO - 10.1016/j.yexcr.2019.05.030
M3 - Article
C2 - 31153924
AN - SCOPUS:85066470937
SN - 0014-4827
VL - 382
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
M1 - 111449
ER -