Spatiotemporal expression pattern of Myt/NZF family zinc finger transcription factors during mouse nervous system development

Fumio Matsushita, Toshiki Kameyama, Yuzo Kadokawa, Tohru Marunouchi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background:: Three members of the Myt/NZF family of transcription factors are involved in many processes of vertebrate development. Several studies have reported that Myt1/NZF-2 has a regulatory function in the development of cultured oligodendrocyte progenitors or in neuronal differentiation during Xenopus primary neurogenesis. However, little is known about the proper function of Myt/NZF family proteins during mammalian nervous system development. To assess the possible function of Myt/NZF transcription factors in mammalian neuronal differentiation, we determined the comparative spatial and temporal expression patterns of all three types of Myt/NZF family genes in the embryonic mouse nervous system using quantitative reverse transcriptase polymerase chain reaction and in situ hybridization. Results: All three Myt/NZF family genes were extensively expressed in developing mouse nervous tissues, and their expression was transient. NZF-1 was expressed later in post-mitotic neurons. NZF-2 was initially expressed in neuronal cells a little earlier than NZF-3. NZF-3 was initially expressed in neuronal cells, just after proliferation was complete. Conclusion: These expression patterns suggest that the expression of NZF family genes is spatially and temporally regulated, and each Myt/NZF family gene may have a regulatory function in a specific phase during neuronal differentiation.

Original languageEnglish
Pages (from-to)588-600
Number of pages13
JournalDevelopmental Dynamics
Volume243
Issue number4
DOIs
Publication statusPublished - 01-01-2014

Fingerprint

Zinc Fingers
Nervous System
Transcription Factors
Genes
Nerve Tissue
Neurogenesis
Oligodendroglia
Xenopus
Reverse Transcriptase Polymerase Chain Reaction
In Situ Hybridization
Vertebrates
Neurons
Proteins

All Science Journal Classification (ASJC) codes

  • Developmental Biology

Cite this

@article{9a086ef38dd445558cff59eaf2ebb2c7,
title = "Spatiotemporal expression pattern of Myt/NZF family zinc finger transcription factors during mouse nervous system development",
abstract = "Background:: Three members of the Myt/NZF family of transcription factors are involved in many processes of vertebrate development. Several studies have reported that Myt1/NZF-2 has a regulatory function in the development of cultured oligodendrocyte progenitors or in neuronal differentiation during Xenopus primary neurogenesis. However, little is known about the proper function of Myt/NZF family proteins during mammalian nervous system development. To assess the possible function of Myt/NZF transcription factors in mammalian neuronal differentiation, we determined the comparative spatial and temporal expression patterns of all three types of Myt/NZF family genes in the embryonic mouse nervous system using quantitative reverse transcriptase polymerase chain reaction and in situ hybridization. Results: All three Myt/NZF family genes were extensively expressed in developing mouse nervous tissues, and their expression was transient. NZF-1 was expressed later in post-mitotic neurons. NZF-2 was initially expressed in neuronal cells a little earlier than NZF-3. NZF-3 was initially expressed in neuronal cells, just after proliferation was complete. Conclusion: These expression patterns suggest that the expression of NZF family genes is spatially and temporally regulated, and each Myt/NZF family gene may have a regulatory function in a specific phase during neuronal differentiation.",
author = "Fumio Matsushita and Toshiki Kameyama and Yuzo Kadokawa and Tohru Marunouchi",
year = "2014",
month = "1",
day = "1",
doi = "10.1002/dvdy.24091",
language = "English",
volume = "243",
pages = "588--600",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "4",

}

Spatiotemporal expression pattern of Myt/NZF family zinc finger transcription factors during mouse nervous system development. / Matsushita, Fumio; Kameyama, Toshiki; Kadokawa, Yuzo; Marunouchi, Tohru.

In: Developmental Dynamics, Vol. 243, No. 4, 01.01.2014, p. 588-600.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Spatiotemporal expression pattern of Myt/NZF family zinc finger transcription factors during mouse nervous system development

AU - Matsushita, Fumio

AU - Kameyama, Toshiki

AU - Kadokawa, Yuzo

AU - Marunouchi, Tohru

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background:: Three members of the Myt/NZF family of transcription factors are involved in many processes of vertebrate development. Several studies have reported that Myt1/NZF-2 has a regulatory function in the development of cultured oligodendrocyte progenitors or in neuronal differentiation during Xenopus primary neurogenesis. However, little is known about the proper function of Myt/NZF family proteins during mammalian nervous system development. To assess the possible function of Myt/NZF transcription factors in mammalian neuronal differentiation, we determined the comparative spatial and temporal expression patterns of all three types of Myt/NZF family genes in the embryonic mouse nervous system using quantitative reverse transcriptase polymerase chain reaction and in situ hybridization. Results: All three Myt/NZF family genes were extensively expressed in developing mouse nervous tissues, and their expression was transient. NZF-1 was expressed later in post-mitotic neurons. NZF-2 was initially expressed in neuronal cells a little earlier than NZF-3. NZF-3 was initially expressed in neuronal cells, just after proliferation was complete. Conclusion: These expression patterns suggest that the expression of NZF family genes is spatially and temporally regulated, and each Myt/NZF family gene may have a regulatory function in a specific phase during neuronal differentiation.

AB - Background:: Three members of the Myt/NZF family of transcription factors are involved in many processes of vertebrate development. Several studies have reported that Myt1/NZF-2 has a regulatory function in the development of cultured oligodendrocyte progenitors or in neuronal differentiation during Xenopus primary neurogenesis. However, little is known about the proper function of Myt/NZF family proteins during mammalian nervous system development. To assess the possible function of Myt/NZF transcription factors in mammalian neuronal differentiation, we determined the comparative spatial and temporal expression patterns of all three types of Myt/NZF family genes in the embryonic mouse nervous system using quantitative reverse transcriptase polymerase chain reaction and in situ hybridization. Results: All three Myt/NZF family genes were extensively expressed in developing mouse nervous tissues, and their expression was transient. NZF-1 was expressed later in post-mitotic neurons. NZF-2 was initially expressed in neuronal cells a little earlier than NZF-3. NZF-3 was initially expressed in neuronal cells, just after proliferation was complete. Conclusion: These expression patterns suggest that the expression of NZF family genes is spatially and temporally regulated, and each Myt/NZF family gene may have a regulatory function in a specific phase during neuronal differentiation.

UR - http://www.scopus.com/inward/record.url?scp=84897088892&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897088892&partnerID=8YFLogxK

U2 - 10.1002/dvdy.24091

DO - 10.1002/dvdy.24091

M3 - Article

C2 - 24214099

AN - SCOPUS:84897088892

VL - 243

SP - 588

EP - 600

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 4

ER -