Spironolactone produces antiacne effects and has recently been shown to inhibit 5α-dihydrotestosterone (5α-DHT) receptors in human sebaceous glands. We applied spironolactone alone and combined with testosterone and 5α-DHT to investigate its effects on the proliferation of human sebocyte cultures derived from facial skin. Secondary human facial sebocytes in 96-well culture plates were treated for 10 d by a single or combined application of testosterone (10-8-10-5M), 5α-DHT (10-8-10-5M), and spironolactone (10-12-10-7 M) in serum-free basal medium. Cell proliferation was assessed in six wells using a fluorometric assay. Testosterone and 5α-DHT significantly stimulated sebocyte proliferation in a dose-dependent manner, the effect being strongest with 5α-DHT. Spironolactone, on the other hand, caused a dose-dependent inhibition (25% and 50% at 10-9 and 10-7 M, respectively). Combined treatment of human facial sebocytes with spironolactone and testosterone or 5α-DHT resulted in a lower proliferation than with androgens alone. The fact that spironolactone directly and dose dependently inhibits the proliferation of cultured human facial sebocytes and acts antagonistically to testosterone and 5α-DHT at the cellular level is indicative of a receptor-mediated effect.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology