Spread of novel aminoglycoside resistance gene aac(6′)-Iad among Acinetobacter clinical isolates in Japan

Yohei Doi, Jun Ichi Wachino, Kunikazu Yamane, Naohiro Shibata, Tetsuya Yagi, Keigo Shibayama, Haru Kato, Yoshichika Arakawa

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A novel aminoglycoside resistance gene, aac(6′)-Iad, encoding aminoglycoside 6′-N-acetyltransferase, was identified in Acinetobacter genospecies 3 strain A.51. The gene encoded a 144-amino-acid protein, which shared modest identity (up to 36.7%) with some of the aminoglycoside 6′-N-acetyltransferases. The results of high-pressure liquid chromatography assays confirmed that the protein is a functional aminoglycoside 6′-N-acetyltransferase. The enzyme conferred resistance to amikacin, tobramycin, sisomicin, and isepamicin but not to gentamicin. The prevalence of this gene among Acinetobacter clinical isolates in Japan was then investigated. Of 264 Acinetobacter sp. strains isolated from geographically diverse areas in Japan in 2002, 16 were not susceptible to amikacin, and aac(6′)-Iad was detected in 7. Five of the producers of aminoglycoside 6′ -N-acetyltransferase type Iad were identified as Acinetobacter baumannii, and two were identified as Acinetobacter genospecies 3. These results suggest that aac(6′)-Iad plays a substantial role in amikacin resistance among Acinetobacter spp. in Japan.

Original languageEnglish
Pages (from-to)2075-2080
Number of pages6
JournalAntimicrobial agents and chemotherapy
Volume48
Issue number6
DOIs
Publication statusPublished - 01-06-2004

Fingerprint

Acinetobacter
Aminoglycosides
Japan
Amikacin
Genes
Sisomicin
Acinetobacter baumannii
Tobramycin
Gentamicins
Proteins
High Pressure Liquid Chromatography
Amino Acids
aminoglycoside N(6')-acetyltransferase
Enzymes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Doi, Yohei ; Wachino, Jun Ichi ; Yamane, Kunikazu ; Shibata, Naohiro ; Yagi, Tetsuya ; Shibayama, Keigo ; Kato, Haru ; Arakawa, Yoshichika. / Spread of novel aminoglycoside resistance gene aac(6′)-Iad among Acinetobacter clinical isolates in Japan. In: Antimicrobial agents and chemotherapy. 2004 ; Vol. 48, No. 6. pp. 2075-2080.
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Spread of novel aminoglycoside resistance gene aac(6′)-Iad among Acinetobacter clinical isolates in Japan. / Doi, Yohei; Wachino, Jun Ichi; Yamane, Kunikazu; Shibata, Naohiro; Yagi, Tetsuya; Shibayama, Keigo; Kato, Haru; Arakawa, Yoshichika.

In: Antimicrobial agents and chemotherapy, Vol. 48, No. 6, 01.06.2004, p. 2075-2080.

Research output: Contribution to journalArticle

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AU - Doi, Yohei

AU - Wachino, Jun Ichi

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AU - Shibata, Naohiro

AU - Yagi, Tetsuya

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AU - Kato, Haru

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