Stability of frozen serum levels of insulin-like growth factor-I, insulin-like growth factor-II, insulin-like growth factor binding protien-3, transforming growth factor β, soluble fas, and superoxide dismutase activity for the JACC Study

Yoshinori Ito, Kei Nakachi, Kazue Imai, Shuji Hashimoto, Yoshiyuki Watanabe, Yutaka Inaba, Akiko Tamakoshi, Takesumi Yoshimura, Mitsuru Mori, Yutaka Motohashi, Ichiro Tsuji, Yosikazu Nakamura, Hiroyasu Iso, Haruo Mikami, Yutaka Inaba, Yoshiharu Hoshiyama, Hiroshi Suzuki, Hiroyuki Shimizu, Hideaki Toyoshima, Shinkan TokudomeShogo Kikuchi, Akio Koizumi, Takashi Kawamura, Tsuneharu Miki, Chigusa Date, Kiyomi Sakata, Takayuki Nose, Norihiko Hayakawa, Akira Shibata, Naoyuki Okamoto, Hideo Shio, Yoshiyuki Ohno, Tomoyuki Kitagawa, Toshio Kuroki, Kazuo Tajima

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Background: Subjects of the Japan Collaborate Cohort Study (JACC Study) gave peripheral blood samples collected between 1988 and 1990. We conducted to investigate whether levels of serum components measured after 9 years of frozen storage are stable or not. Methods: To assess the degradation of frozen serum components in the JACC Study, we compared levels of various components (IGF-I, IGF-II, IGFBP-3, TGF-β1, sFas, and total SOD activity) between fresh and stored sera collected from other inhabitants. Serum levels of constituents were measured by immunoradiometric assay (IGF-I, IGF-II and IGFBP-3), quantitative enzyme immunciassay (TGF-β1), enzyme-linked immuno-adsorbent assay (sFas), and an improved nitrite method (SOD activity). Results: The coefficients of variation for intra- and inter-assay precisions of the measurements were less than 9%. Levels of IGF-I, IGF-11, IGFBP-3, TGF-β1 and sFas in sera after storage for 9 years at - 80°C were similar to those of fresh sera newly collected from inhabitants. The distributions of serum IGF-I, IGF-II, IGFBP-3, TGF-β1, sFas and SOD activity for specimens collected from different individuals tended to be similar to those of serum levels for frozen specimens collected from different individuals and stored for 9 years. Conclusions: There was no statistically significant difference in distribution of measured values of IGF-I, IGF-II, IGFBP-3, TGF-β1, and sFas between newly collected sera and frozen specimens stored for 9 years. Thus, measurements of these serum constituents of specimens stored for the JACC Study can be reliably used in nested case-control study.

Original languageEnglish
JournalJournal of epidemiology
Volume15
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 12-10-2005

Fingerprint

Insulin-Like Growth Factor II
Transforming Growth Factors
Somatomedins
Insulin-Like Growth Factor I
Superoxide Dismutase
Japan
Cohort Studies
Insulin-Like Growth Factor Binding Protein 3
Serum
Immunoradiometric Assay
Enzymes
Nitrites
Case-Control Studies

All Science Journal Classification (ASJC) codes

  • Epidemiology

Cite this

Ito, Yoshinori ; Nakachi, Kei ; Imai, Kazue ; Hashimoto, Shuji ; Watanabe, Yoshiyuki ; Inaba, Yutaka ; Tamakoshi, Akiko ; Yoshimura, Takesumi ; Mori, Mitsuru ; Motohashi, Yutaka ; Tsuji, Ichiro ; Nakamura, Yosikazu ; Iso, Hiroyasu ; Mikami, Haruo ; Inaba, Yutaka ; Hoshiyama, Yoshiharu ; Suzuki, Hiroshi ; Shimizu, Hiroyuki ; Toyoshima, Hideaki ; Tokudome, Shinkan ; Kikuchi, Shogo ; Koizumi, Akio ; Kawamura, Takashi ; Miki, Tsuneharu ; Date, Chigusa ; Sakata, Kiyomi ; Nose, Takayuki ; Hayakawa, Norihiko ; Shibata, Akira ; Okamoto, Naoyuki ; Shio, Hideo ; Ohno, Yoshiyuki ; Kitagawa, Tomoyuki ; Kuroki, Toshio ; Tajima, Kazuo. / Stability of frozen serum levels of insulin-like growth factor-I, insulin-like growth factor-II, insulin-like growth factor binding protien-3, transforming growth factor β, soluble fas, and superoxide dismutase activity for the JACC Study. In: Journal of epidemiology. 2005 ; Vol. 15, No. SUPPL. 1.
@article{c66a38b7217b46758da61ea5c5311550,
title = "Stability of frozen serum levels of insulin-like growth factor-I, insulin-like growth factor-II, insulin-like growth factor binding protien-3, transforming growth factor β, soluble fas, and superoxide dismutase activity for the JACC Study",
abstract = "Background: Subjects of the Japan Collaborate Cohort Study (JACC Study) gave peripheral blood samples collected between 1988 and 1990. We conducted to investigate whether levels of serum components measured after 9 years of frozen storage are stable or not. Methods: To assess the degradation of frozen serum components in the JACC Study, we compared levels of various components (IGF-I, IGF-II, IGFBP-3, TGF-β1, sFas, and total SOD activity) between fresh and stored sera collected from other inhabitants. Serum levels of constituents were measured by immunoradiometric assay (IGF-I, IGF-II and IGFBP-3), quantitative enzyme immunciassay (TGF-β1), enzyme-linked immuno-adsorbent assay (sFas), and an improved nitrite method (SOD activity). Results: The coefficients of variation for intra- and inter-assay precisions of the measurements were less than 9{\%}. Levels of IGF-I, IGF-11, IGFBP-3, TGF-β1 and sFas in sera after storage for 9 years at - 80°C were similar to those of fresh sera newly collected from inhabitants. The distributions of serum IGF-I, IGF-II, IGFBP-3, TGF-β1, sFas and SOD activity for specimens collected from different individuals tended to be similar to those of serum levels for frozen specimens collected from different individuals and stored for 9 years. Conclusions: There was no statistically significant difference in distribution of measured values of IGF-I, IGF-II, IGFBP-3, TGF-β1, and sFas between newly collected sera and frozen specimens stored for 9 years. Thus, measurements of these serum constituents of specimens stored for the JACC Study can be reliably used in nested case-control study.",
author = "Yoshinori Ito and Kei Nakachi and Kazue Imai and Shuji Hashimoto and Yoshiyuki Watanabe and Yutaka Inaba and Akiko Tamakoshi and Takesumi Yoshimura and Mitsuru Mori and Yutaka Motohashi and Ichiro Tsuji and Yosikazu Nakamura and Hiroyasu Iso and Haruo Mikami and Yutaka Inaba and Yoshiharu Hoshiyama and Hiroshi Suzuki and Hiroyuki Shimizu and Hideaki Toyoshima and Shinkan Tokudome and Shogo Kikuchi and Akio Koizumi and Takashi Kawamura and Tsuneharu Miki and Chigusa Date and Kiyomi Sakata and Takayuki Nose and Norihiko Hayakawa and Akira Shibata and Naoyuki Okamoto and Hideo Shio and Yoshiyuki Ohno and Tomoyuki Kitagawa and Toshio Kuroki and Kazuo Tajima",
year = "2005",
month = "10",
day = "12",
doi = "10.2188/jea.15.S67",
language = "English",
volume = "15",
journal = "Journal of Epidemiology",
issn = "0917-5040",
publisher = "Japan Epidemiology Association",
number = "SUPPL. 1",

}

Ito, Y, Nakachi, K, Imai, K, Hashimoto, S, Watanabe, Y, Inaba, Y, Tamakoshi, A, Yoshimura, T, Mori, M, Motohashi, Y, Tsuji, I, Nakamura, Y, Iso, H, Mikami, H, Inaba, Y, Hoshiyama, Y, Suzuki, H, Shimizu, H, Toyoshima, H, Tokudome, S, Kikuchi, S, Koizumi, A, Kawamura, T, Miki, T, Date, C, Sakata, K, Nose, T, Hayakawa, N, Shibata, A, Okamoto, N, Shio, H, Ohno, Y, Kitagawa, T, Kuroki, T & Tajima, K 2005, 'Stability of frozen serum levels of insulin-like growth factor-I, insulin-like growth factor-II, insulin-like growth factor binding protien-3, transforming growth factor β, soluble fas, and superoxide dismutase activity for the JACC Study', Journal of epidemiology, vol. 15, no. SUPPL. 1. https://doi.org/10.2188/jea.15.S67

Stability of frozen serum levels of insulin-like growth factor-I, insulin-like growth factor-II, insulin-like growth factor binding protien-3, transforming growth factor β, soluble fas, and superoxide dismutase activity for the JACC Study. / Ito, Yoshinori; Nakachi, Kei; Imai, Kazue; Hashimoto, Shuji; Watanabe, Yoshiyuki; Inaba, Yutaka; Tamakoshi, Akiko; Yoshimura, Takesumi; Mori, Mitsuru; Motohashi, Yutaka; Tsuji, Ichiro; Nakamura, Yosikazu; Iso, Hiroyasu; Mikami, Haruo; Inaba, Yutaka; Hoshiyama, Yoshiharu; Suzuki, Hiroshi; Shimizu, Hiroyuki; Toyoshima, Hideaki; Tokudome, Shinkan; Kikuchi, Shogo; Koizumi, Akio; Kawamura, Takashi; Miki, Tsuneharu; Date, Chigusa; Sakata, Kiyomi; Nose, Takayuki; Hayakawa, Norihiko; Shibata, Akira; Okamoto, Naoyuki; Shio, Hideo; Ohno, Yoshiyuki; Kitagawa, Tomoyuki; Kuroki, Toshio; Tajima, Kazuo.

In: Journal of epidemiology, Vol. 15, No. SUPPL. 1, 12.10.2005.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Stability of frozen serum levels of insulin-like growth factor-I, insulin-like growth factor-II, insulin-like growth factor binding protien-3, transforming growth factor β, soluble fas, and superoxide dismutase activity for the JACC Study

AU - Ito, Yoshinori

AU - Nakachi, Kei

AU - Imai, Kazue

AU - Hashimoto, Shuji

AU - Watanabe, Yoshiyuki

AU - Inaba, Yutaka

AU - Tamakoshi, Akiko

AU - Yoshimura, Takesumi

AU - Mori, Mitsuru

AU - Motohashi, Yutaka

AU - Tsuji, Ichiro

AU - Nakamura, Yosikazu

AU - Iso, Hiroyasu

AU - Mikami, Haruo

AU - Inaba, Yutaka

AU - Hoshiyama, Yoshiharu

AU - Suzuki, Hiroshi

AU - Shimizu, Hiroyuki

AU - Toyoshima, Hideaki

AU - Tokudome, Shinkan

AU - Kikuchi, Shogo

AU - Koizumi, Akio

AU - Kawamura, Takashi

AU - Miki, Tsuneharu

AU - Date, Chigusa

AU - Sakata, Kiyomi

AU - Nose, Takayuki

AU - Hayakawa, Norihiko

AU - Shibata, Akira

AU - Okamoto, Naoyuki

AU - Shio, Hideo

AU - Ohno, Yoshiyuki

AU - Kitagawa, Tomoyuki

AU - Kuroki, Toshio

AU - Tajima, Kazuo

PY - 2005/10/12

Y1 - 2005/10/12

N2 - Background: Subjects of the Japan Collaborate Cohort Study (JACC Study) gave peripheral blood samples collected between 1988 and 1990. We conducted to investigate whether levels of serum components measured after 9 years of frozen storage are stable or not. Methods: To assess the degradation of frozen serum components in the JACC Study, we compared levels of various components (IGF-I, IGF-II, IGFBP-3, TGF-β1, sFas, and total SOD activity) between fresh and stored sera collected from other inhabitants. Serum levels of constituents were measured by immunoradiometric assay (IGF-I, IGF-II and IGFBP-3), quantitative enzyme immunciassay (TGF-β1), enzyme-linked immuno-adsorbent assay (sFas), and an improved nitrite method (SOD activity). Results: The coefficients of variation for intra- and inter-assay precisions of the measurements were less than 9%. Levels of IGF-I, IGF-11, IGFBP-3, TGF-β1 and sFas in sera after storage for 9 years at - 80°C were similar to those of fresh sera newly collected from inhabitants. The distributions of serum IGF-I, IGF-II, IGFBP-3, TGF-β1, sFas and SOD activity for specimens collected from different individuals tended to be similar to those of serum levels for frozen specimens collected from different individuals and stored for 9 years. Conclusions: There was no statistically significant difference in distribution of measured values of IGF-I, IGF-II, IGFBP-3, TGF-β1, and sFas between newly collected sera and frozen specimens stored for 9 years. Thus, measurements of these serum constituents of specimens stored for the JACC Study can be reliably used in nested case-control study.

AB - Background: Subjects of the Japan Collaborate Cohort Study (JACC Study) gave peripheral blood samples collected between 1988 and 1990. We conducted to investigate whether levels of serum components measured after 9 years of frozen storage are stable or not. Methods: To assess the degradation of frozen serum components in the JACC Study, we compared levels of various components (IGF-I, IGF-II, IGFBP-3, TGF-β1, sFas, and total SOD activity) between fresh and stored sera collected from other inhabitants. Serum levels of constituents were measured by immunoradiometric assay (IGF-I, IGF-II and IGFBP-3), quantitative enzyme immunciassay (TGF-β1), enzyme-linked immuno-adsorbent assay (sFas), and an improved nitrite method (SOD activity). Results: The coefficients of variation for intra- and inter-assay precisions of the measurements were less than 9%. Levels of IGF-I, IGF-11, IGFBP-3, TGF-β1 and sFas in sera after storage for 9 years at - 80°C were similar to those of fresh sera newly collected from inhabitants. The distributions of serum IGF-I, IGF-II, IGFBP-3, TGF-β1, sFas and SOD activity for specimens collected from different individuals tended to be similar to those of serum levels for frozen specimens collected from different individuals and stored for 9 years. Conclusions: There was no statistically significant difference in distribution of measured values of IGF-I, IGF-II, IGFBP-3, TGF-β1, and sFas between newly collected sera and frozen specimens stored for 9 years. Thus, measurements of these serum constituents of specimens stored for the JACC Study can be reliably used in nested case-control study.

UR - http://www.scopus.com/inward/record.url?scp=19444366925&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19444366925&partnerID=8YFLogxK

U2 - 10.2188/jea.15.S67

DO - 10.2188/jea.15.S67

M3 - Article

C2 - 15881197

AN - SCOPUS:19444366925

VL - 15

JO - Journal of Epidemiology

JF - Journal of Epidemiology

SN - 0917-5040

IS - SUPPL. 1

ER -