Stability of serum high-density lipoprotein-microRNAs for preanalytical conditions

Hiroaki Ishikawa, Hiroya Yamada, Nao Taromaru, Kanako Kondo, Ayuri Nagura, Mirai Yamazaki, Yoshitaka Ando, Eiji Munetsuna, Koji Suzuki, Koji Ohashi, Ryoji Teradaira

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Recently, several studies have shown that microRNAs are present in high-density lipoprotein, and high-density lipoprotein-microRNA may be a promising disease biomarker. We investigated the stability of high-density lipoprotein-microRNAs in different storage conditions as this is an important issue for its application to the field of clinical research. Methods: microRNAs were extracted from the high-density lipoprotein fraction that was purified from the serum. miR-135 a and miR-223, which are known to be present in high-density lipoprotein, were quantified by quantitative real-time PCR. The influence of preanalytical parameters on the analysis of high-density lipoprotein-miRNAs was examined by the effect of RNase, storage conditions, and freezing and thawing. Results: The concentrations of microRNA in high-density lipoprotein were not altered by RNase A treatment (0–100 U/mL). No significant change in these microRNAs was observed after storing serum at room temperature or 4℃ for 0–24 h, and there was a similar result in the cryopreservation for up to two weeks. Also, high-density lipoprotein-microRNAs were stable for, at least, up to five freeze–thaw cycles. Conclusions: These results demonstrated that high-density lipoprotein-microRNAs are relatively resistant to various storage conditions. This study provides new and important information on the stability of high-density lipoprotein-microRNAs.

Original languageEnglish
Pages (from-to)134-142
Number of pages9
JournalAnnals of Clinical Biochemistry
Volume54
Issue number1
DOIs
Publication statusPublished - 01-01-2017

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HDL Lipoproteins
MicroRNAs
Serum
Pancreatic Ribonuclease
Thawing
Cryopreservation
Biomarkers
Ribonucleases
Freezing
Real-Time Polymerase Chain Reaction
Temperature

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Ishikawa, Hiroaki ; Yamada, Hiroya ; Taromaru, Nao ; Kondo, Kanako ; Nagura, Ayuri ; Yamazaki, Mirai ; Ando, Yoshitaka ; Munetsuna, Eiji ; Suzuki, Koji ; Ohashi, Koji ; Teradaira, Ryoji. / Stability of serum high-density lipoprotein-microRNAs for preanalytical conditions. In: Annals of Clinical Biochemistry. 2017 ; Vol. 54, No. 1. pp. 134-142.
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Stability of serum high-density lipoprotein-microRNAs for preanalytical conditions. / Ishikawa, Hiroaki; Yamada, Hiroya; Taromaru, Nao; Kondo, Kanako; Nagura, Ayuri; Yamazaki, Mirai; Ando, Yoshitaka; Munetsuna, Eiji; Suzuki, Koji; Ohashi, Koji; Teradaira, Ryoji.

In: Annals of Clinical Biochemistry, Vol. 54, No. 1, 01.01.2017, p. 134-142.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Stability of serum high-density lipoprotein-microRNAs for preanalytical conditions

AU - Ishikawa, Hiroaki

AU - Yamada, Hiroya

AU - Taromaru, Nao

AU - Kondo, Kanako

AU - Nagura, Ayuri

AU - Yamazaki, Mirai

AU - Ando, Yoshitaka

AU - Munetsuna, Eiji

AU - Suzuki, Koji

AU - Ohashi, Koji

AU - Teradaira, Ryoji

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Background: Recently, several studies have shown that microRNAs are present in high-density lipoprotein, and high-density lipoprotein-microRNA may be a promising disease biomarker. We investigated the stability of high-density lipoprotein-microRNAs in different storage conditions as this is an important issue for its application to the field of clinical research. Methods: microRNAs were extracted from the high-density lipoprotein fraction that was purified from the serum. miR-135 a and miR-223, which are known to be present in high-density lipoprotein, were quantified by quantitative real-time PCR. The influence of preanalytical parameters on the analysis of high-density lipoprotein-miRNAs was examined by the effect of RNase, storage conditions, and freezing and thawing. Results: The concentrations of microRNA in high-density lipoprotein were not altered by RNase A treatment (0–100 U/mL). No significant change in these microRNAs was observed after storing serum at room temperature or 4℃ for 0–24 h, and there was a similar result in the cryopreservation for up to two weeks. Also, high-density lipoprotein-microRNAs were stable for, at least, up to five freeze–thaw cycles. Conclusions: These results demonstrated that high-density lipoprotein-microRNAs are relatively resistant to various storage conditions. This study provides new and important information on the stability of high-density lipoprotein-microRNAs.

AB - Background: Recently, several studies have shown that microRNAs are present in high-density lipoprotein, and high-density lipoprotein-microRNA may be a promising disease biomarker. We investigated the stability of high-density lipoprotein-microRNAs in different storage conditions as this is an important issue for its application to the field of clinical research. Methods: microRNAs were extracted from the high-density lipoprotein fraction that was purified from the serum. miR-135 a and miR-223, which are known to be present in high-density lipoprotein, were quantified by quantitative real-time PCR. The influence of preanalytical parameters on the analysis of high-density lipoprotein-miRNAs was examined by the effect of RNase, storage conditions, and freezing and thawing. Results: The concentrations of microRNA in high-density lipoprotein were not altered by RNase A treatment (0–100 U/mL). No significant change in these microRNAs was observed after storing serum at room temperature or 4℃ for 0–24 h, and there was a similar result in the cryopreservation for up to two weeks. Also, high-density lipoprotein-microRNAs were stable for, at least, up to five freeze–thaw cycles. Conclusions: These results demonstrated that high-density lipoprotein-microRNAs are relatively resistant to various storage conditions. This study provides new and important information on the stability of high-density lipoprotein-microRNAs.

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