Stable expression of glutathione S-transferase P-form mRNA and TGF-α immunohistochemical expression are useful markers for identification of persistent nodules from an early stage in rat hepatocarcinogenesis

Toshio Imai, Toshifumi Tashiro, Kazuhiro Hayakawa, Junichi Nakanowatari, Taneo Fukuta, Satoru Hosokawa, Tetsuya Tsukamoto, Ken Ichi Inada, Masae Tatematsu

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Expression of glutathione S-transferase P-form (GST-P) mRNA revealed by in situ hybridization histochemistry was investigated in enzyme altered foci and nodules during rat hepatocarcinogenesis, using diethylnitrosamine initiation followed by 2-acetylaminofluorene promotion protocol. The majority of early foci showed uniformly high levels of GST-P mRNA, however, some lesions after completion of the promotion regimen exhibited a progressive loss of expression of GSTP mRNA. Most of the continuous and strong GST-P mRNA positive lesions were also transforming growth factor-α (TGFα)-positive lesions, a putative potent mitogen for altered hepatocytes. Moreover, the proliferating cell nuclear antigen (PCNA) index was significantly (p<0.05) greater in the GST-P mRNA-positive nodules than that in negative ones. These results suggest that stable expression of GST-P mRNA is a very useful marker for identification of developing preneoplastic lesions during rat hepatocarcinogenesis.

Original languageEnglish
Pages (from-to)249-255
Number of pages7
JournalJournal of Toxicologic Pathology
Volume11
Issue number4
DOIs
Publication statusPublished - 1998

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Toxicology

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