TY - JOUR
T1 - Stem cells from human exfoliated deciduous teeth enhance recovery from focal cerebral ischemia in rats
AU - Sugiyama, Masahiko
AU - Hattori, Hisashi
AU - Inoue, Takanori
AU - Wakita, Hideaki
AU - Hibi, Hideharu
AU - Ueda, Minoru
N1 - Publisher Copyright:
© 2013 Asian AOMS, ASOMP, JSOP, JSOMS, JSOM, and JAMI.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Purpose: The aim of this study was to investigate the effect of stem cells from human exfoliated deciduous teeth (SHED) after permanent MCAO (pMCAO). Materials and methods: Adult male Sprague-Dawley rats were subjected to pMCAO. After pMCAO, SHED was transplanted into the brain. Motor function and infarct volume were evaluated. Neurogenesis and vasculogenesis were determined using immunochemical markers. Results: The SHED group had more positive signals for doublecortin, neurofilament, anti-neuronal nuclei (NeuN) and rat endothelial cell antigen-1 (RECA1) in the peri-infarct area than the PBS group. Migration of doublecortin-positive neural progenitor cells (NPCs) from subventricular zone (SVZ) to the peri-infarct area was observed on day 16. Transplanted SHED merged vascular endothelial growth factor (VEGF) and stromal cell-derived factor 1 (SDF-1) positive cells. Conclusion: SHED promoted migration and differentiation of the endogenous NPCs and induced vasculogenesis, and ameliorated ischemic brain injury after pMCAO in rats.
AB - Purpose: The aim of this study was to investigate the effect of stem cells from human exfoliated deciduous teeth (SHED) after permanent MCAO (pMCAO). Materials and methods: Adult male Sprague-Dawley rats were subjected to pMCAO. After pMCAO, SHED was transplanted into the brain. Motor function and infarct volume were evaluated. Neurogenesis and vasculogenesis were determined using immunochemical markers. Results: The SHED group had more positive signals for doublecortin, neurofilament, anti-neuronal nuclei (NeuN) and rat endothelial cell antigen-1 (RECA1) in the peri-infarct area than the PBS group. Migration of doublecortin-positive neural progenitor cells (NPCs) from subventricular zone (SVZ) to the peri-infarct area was observed on day 16. Transplanted SHED merged vascular endothelial growth factor (VEGF) and stromal cell-derived factor 1 (SDF-1) positive cells. Conclusion: SHED promoted migration and differentiation of the endogenous NPCs and induced vasculogenesis, and ameliorated ischemic brain injury after pMCAO in rats.
KW - Cerebral ischemia
KW - Dental pulp stem cells
KW - Neurogenesis
KW - Stem cells from human exfoliated deciduous teeth
UR - http://www.scopus.com/inward/record.url?scp=84908328680&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84908328680&partnerID=8YFLogxK
U2 - 10.1016/j.ajoms.2013.04.014
DO - 10.1016/j.ajoms.2013.04.014
M3 - Article
AN - SCOPUS:84908328680
SN - 2212-5558
VL - 26
SP - 443
EP - 449
JO - Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology
JF - Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology
IS - 4
ER -