TY - JOUR
T1 - Stimulated neuronal expression of brain-derived neurotrophic factor by Neurotropin
AU - Fukuda, Yu
AU - Berry, Thomas L.
AU - Nelson, Matthew
AU - Hunter, Christopher L.
AU - Fukuhara, Koki
AU - Imai, Hideki
AU - Ito, Shinji
AU - Granholm-Bentley, Ann Charlotte
AU - Kaplan, Allen P.
AU - Mutoh, Tatsuro
PY - 2010/11
Y1 - 2010/11
N2 - Expression of brain-derived neurotrophic factor (BDNF) was stimulated in human neuroblastoma SH-SY5Y cells by a nonprotein extract of inflamed rabbit skin inoculated with vaccinia virus (Neurotropin®), an analgesic widely used in Japan for treatment of disorders associated with chronic pain, with the optimal dosage at 10. mNU/mL. This stimulation was accompanied by activations of p42/44 MAP kinase, CREB and c-Fos expression. Inhibitors of MAP kinases or PI 3-kinase prevented the stimulatory action of Neurotropin, indicating that neuronal TrkB/CREB pathway mediates the action. Repetitive oral administration of Neurotropin (200. NU/kg/day, 3. months) prevented the age-dependent decline in hippocampal BDNF expression in Ts65Dn mice, a model of Down's syndrome. This effect was associated with the improvement of spatial cognition of the mice. These results open an intriguing new strategy in which Neurotropin may prove beneficial treatment for neurodegenerative disorders.
AB - Expression of brain-derived neurotrophic factor (BDNF) was stimulated in human neuroblastoma SH-SY5Y cells by a nonprotein extract of inflamed rabbit skin inoculated with vaccinia virus (Neurotropin®), an analgesic widely used in Japan for treatment of disorders associated with chronic pain, with the optimal dosage at 10. mNU/mL. This stimulation was accompanied by activations of p42/44 MAP kinase, CREB and c-Fos expression. Inhibitors of MAP kinases or PI 3-kinase prevented the stimulatory action of Neurotropin, indicating that neuronal TrkB/CREB pathway mediates the action. Repetitive oral administration of Neurotropin (200. NU/kg/day, 3. months) prevented the age-dependent decline in hippocampal BDNF expression in Ts65Dn mice, a model of Down's syndrome. This effect was associated with the improvement of spatial cognition of the mice. These results open an intriguing new strategy in which Neurotropin may prove beneficial treatment for neurodegenerative disorders.
UR - http://www.scopus.com/inward/record.url?scp=77956616429&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77956616429&partnerID=8YFLogxK
U2 - 10.1016/j.mcn.2010.06.013
DO - 10.1016/j.mcn.2010.06.013
M3 - Article
C2 - 20600926
AN - SCOPUS:77956616429
SN - 1044-7431
VL - 45
SP - 226
EP - 233
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 3
ER -
