Stimulated neuronal expression of brain-derived neurotrophic factor by Neurotropin

Yu Fukuda, Thomas L. Berry, Matthew Nelson, Christopher L. Hunter, Koki Fukuhara, Hideki Imai, Shinji Ito, Ann Charlotte Granholm-Bentley, Allen P. Kaplan, Tatsuro Mutoh

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Expression of brain-derived neurotrophic factor (BDNF) was stimulated in human neuroblastoma SH-SY5Y cells by a nonprotein extract of inflamed rabbit skin inoculated with vaccinia virus (Neurotropin®), an analgesic widely used in Japan for treatment of disorders associated with chronic pain, with the optimal dosage at 10. mNU/mL. This stimulation was accompanied by activations of p42/44 MAP kinase, CREB and c-Fos expression. Inhibitors of MAP kinases or PI 3-kinase prevented the stimulatory action of Neurotropin, indicating that neuronal TrkB/CREB pathway mediates the action. Repetitive oral administration of Neurotropin (200. NU/kg/day, 3. months) prevented the age-dependent decline in hippocampal BDNF expression in Ts65Dn mice, a model of Down's syndrome. This effect was associated with the improvement of spatial cognition of the mice. These results open an intriguing new strategy in which Neurotropin may prove beneficial treatment for neurodegenerative disorders.

Original languageEnglish
Pages (from-to)226-233
Number of pages8
JournalMolecular and Cellular Neuroscience
Volume45
Issue number3
DOIs
Publication statusPublished - 01-11-2010

Fingerprint

Brain-Derived Neurotrophic Factor
Vaccinia virus
Mitogen-Activated Protein Kinase 1
Down Syndrome
Neuroblastoma
Phosphatidylinositol 3-Kinases
Chronic Pain
Neurodegenerative Diseases
Cognition
Oral Administration
Analgesics
Japan
Phosphotransferases
Rabbits
Skin
neurotropin
Therapeutics

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Fukuda, Y., Berry, T. L., Nelson, M., Hunter, C. L., Fukuhara, K., Imai, H., ... Mutoh, T. (2010). Stimulated neuronal expression of brain-derived neurotrophic factor by Neurotropin. Molecular and Cellular Neuroscience, 45(3), 226-233. https://doi.org/10.1016/j.mcn.2010.06.013
Fukuda, Yu ; Berry, Thomas L. ; Nelson, Matthew ; Hunter, Christopher L. ; Fukuhara, Koki ; Imai, Hideki ; Ito, Shinji ; Granholm-Bentley, Ann Charlotte ; Kaplan, Allen P. ; Mutoh, Tatsuro. / Stimulated neuronal expression of brain-derived neurotrophic factor by Neurotropin. In: Molecular and Cellular Neuroscience. 2010 ; Vol. 45, No. 3. pp. 226-233.
@article{d583330532814af5bc56dbc475d4561e,
title = "Stimulated neuronal expression of brain-derived neurotrophic factor by Neurotropin",
abstract = "Expression of brain-derived neurotrophic factor (BDNF) was stimulated in human neuroblastoma SH-SY5Y cells by a nonprotein extract of inflamed rabbit skin inoculated with vaccinia virus (Neurotropin{\circledR}), an analgesic widely used in Japan for treatment of disorders associated with chronic pain, with the optimal dosage at 10. mNU/mL. This stimulation was accompanied by activations of p42/44 MAP kinase, CREB and c-Fos expression. Inhibitors of MAP kinases or PI 3-kinase prevented the stimulatory action of Neurotropin, indicating that neuronal TrkB/CREB pathway mediates the action. Repetitive oral administration of Neurotropin (200. NU/kg/day, 3. months) prevented the age-dependent decline in hippocampal BDNF expression in Ts65Dn mice, a model of Down's syndrome. This effect was associated with the improvement of spatial cognition of the mice. These results open an intriguing new strategy in which Neurotropin may prove beneficial treatment for neurodegenerative disorders.",
author = "Yu Fukuda and Berry, {Thomas L.} and Matthew Nelson and Hunter, {Christopher L.} and Koki Fukuhara and Hideki Imai and Shinji Ito and Granholm-Bentley, {Ann Charlotte} and Kaplan, {Allen P.} and Tatsuro Mutoh",
year = "2010",
month = "11",
day = "1",
doi = "10.1016/j.mcn.2010.06.013",
language = "English",
volume = "45",
pages = "226--233",
journal = "Molecular and Cellular Neurosciences",
issn = "1044-7431",
publisher = "Academic Press Inc.",
number = "3",

}

Fukuda, Y, Berry, TL, Nelson, M, Hunter, CL, Fukuhara, K, Imai, H, Ito, S, Granholm-Bentley, AC, Kaplan, AP & Mutoh, T 2010, 'Stimulated neuronal expression of brain-derived neurotrophic factor by Neurotropin', Molecular and Cellular Neuroscience, vol. 45, no. 3, pp. 226-233. https://doi.org/10.1016/j.mcn.2010.06.013

Stimulated neuronal expression of brain-derived neurotrophic factor by Neurotropin. / Fukuda, Yu; Berry, Thomas L.; Nelson, Matthew; Hunter, Christopher L.; Fukuhara, Koki; Imai, Hideki; Ito, Shinji; Granholm-Bentley, Ann Charlotte; Kaplan, Allen P.; Mutoh, Tatsuro.

In: Molecular and Cellular Neuroscience, Vol. 45, No. 3, 01.11.2010, p. 226-233.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Stimulated neuronal expression of brain-derived neurotrophic factor by Neurotropin

AU - Fukuda, Yu

AU - Berry, Thomas L.

AU - Nelson, Matthew

AU - Hunter, Christopher L.

AU - Fukuhara, Koki

AU - Imai, Hideki

AU - Ito, Shinji

AU - Granholm-Bentley, Ann Charlotte

AU - Kaplan, Allen P.

AU - Mutoh, Tatsuro

PY - 2010/11/1

Y1 - 2010/11/1

N2 - Expression of brain-derived neurotrophic factor (BDNF) was stimulated in human neuroblastoma SH-SY5Y cells by a nonprotein extract of inflamed rabbit skin inoculated with vaccinia virus (Neurotropin®), an analgesic widely used in Japan for treatment of disorders associated with chronic pain, with the optimal dosage at 10. mNU/mL. This stimulation was accompanied by activations of p42/44 MAP kinase, CREB and c-Fos expression. Inhibitors of MAP kinases or PI 3-kinase prevented the stimulatory action of Neurotropin, indicating that neuronal TrkB/CREB pathway mediates the action. Repetitive oral administration of Neurotropin (200. NU/kg/day, 3. months) prevented the age-dependent decline in hippocampal BDNF expression in Ts65Dn mice, a model of Down's syndrome. This effect was associated with the improvement of spatial cognition of the mice. These results open an intriguing new strategy in which Neurotropin may prove beneficial treatment for neurodegenerative disorders.

AB - Expression of brain-derived neurotrophic factor (BDNF) was stimulated in human neuroblastoma SH-SY5Y cells by a nonprotein extract of inflamed rabbit skin inoculated with vaccinia virus (Neurotropin®), an analgesic widely used in Japan for treatment of disorders associated with chronic pain, with the optimal dosage at 10. mNU/mL. This stimulation was accompanied by activations of p42/44 MAP kinase, CREB and c-Fos expression. Inhibitors of MAP kinases or PI 3-kinase prevented the stimulatory action of Neurotropin, indicating that neuronal TrkB/CREB pathway mediates the action. Repetitive oral administration of Neurotropin (200. NU/kg/day, 3. months) prevented the age-dependent decline in hippocampal BDNF expression in Ts65Dn mice, a model of Down's syndrome. This effect was associated with the improvement of spatial cognition of the mice. These results open an intriguing new strategy in which Neurotropin may prove beneficial treatment for neurodegenerative disorders.

UR - http://www.scopus.com/inward/record.url?scp=77956616429&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956616429&partnerID=8YFLogxK

U2 - 10.1016/j.mcn.2010.06.013

DO - 10.1016/j.mcn.2010.06.013

M3 - Article

C2 - 20600926

AN - SCOPUS:77956616429

VL - 45

SP - 226

EP - 233

JO - Molecular and Cellular Neurosciences

JF - Molecular and Cellular Neurosciences

SN - 1044-7431

IS - 3

ER -