Stimulation of estradiol biosynthesis by tributyltin in rat hippocampal slices

Eiji Munetsuna, Minoru Hattori, Takeshi Yamazaki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Hippocampal functions are influenced by steroid hormones, such as testosterone and estradiol. It has been demonstrated that hippocampus-derived steroid hormones play important roles in neuronal protection and synapse formation. Our research groups have demonstrated that estradiol is de novo synthesized in the rat hippocampus. However, the mechanism(s) regulating this synthesis remains unclear. It has been reported that tributyltin, an environmental pollutant, binds to the retinoid X receptor (RXR) and modifies estrogen synthesis in human granulosa-like tumor cells. This compound can penetrate the blood brain barrier, and tends to accumulate in the brain. Based on these facts, we hypothesized that tributyltin could influence the hippocampal estradiol synthesis. A concentration of 0.1μM tributyltin induced an increase in the mRNA content of P450(17α) and P450arom in hippocampal slices, as determined using real-time PCR. The transcript levels of other steroidogenic enzymes and a steroidogenic acute regulatory protein were not affected. The estradiol level in rat hippocampal slices was subsequently determined using a radioimmunoassay. We found that the estradiol synthesis was stimulated by ∼2-fold following a 48-h treatment with 0.1μM tributyltin, and this was accompanied by transcriptional activation of P450(17α) and P450arom. Tributyltin stimulated de novo hippocampal estradiol synthesis by modifying the transcription of specific steroidogenic enzymes.

Original languageEnglish
Pages (from-to)168-172
Number of pages5
JournalEndocrine Research
Volume39
Issue number4
DOIs
Publication statusPublished - 11-01-2014

Fingerprint

Estradiol
Hippocampus
Steroids
Hormones
Granulosa Cell Tumor
Retinoid X Receptors
Environmental Pollutants
Enzymes
Blood-Brain Barrier
Synapses
Transcriptional Activation
Radioimmunoassay
Testosterone
tributyltin
Real-Time Polymerase Chain Reaction
Estrogens
Messenger RNA
Brain
Research

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Munetsuna, Eiji ; Hattori, Minoru ; Yamazaki, Takeshi. / Stimulation of estradiol biosynthesis by tributyltin in rat hippocampal slices. In: Endocrine Research. 2014 ; Vol. 39, No. 4. pp. 168-172.
@article{c748696322a24181bf67a19a326e5f37,
title = "Stimulation of estradiol biosynthesis by tributyltin in rat hippocampal slices",
abstract = "Hippocampal functions are influenced by steroid hormones, such as testosterone and estradiol. It has been demonstrated that hippocampus-derived steroid hormones play important roles in neuronal protection and synapse formation. Our research groups have demonstrated that estradiol is de novo synthesized in the rat hippocampus. However, the mechanism(s) regulating this synthesis remains unclear. It has been reported that tributyltin, an environmental pollutant, binds to the retinoid X receptor (RXR) and modifies estrogen synthesis in human granulosa-like tumor cells. This compound can penetrate the blood brain barrier, and tends to accumulate in the brain. Based on these facts, we hypothesized that tributyltin could influence the hippocampal estradiol synthesis. A concentration of 0.1μM tributyltin induced an increase in the mRNA content of P450(17α) and P450arom in hippocampal slices, as determined using real-time PCR. The transcript levels of other steroidogenic enzymes and a steroidogenic acute regulatory protein were not affected. The estradiol level in rat hippocampal slices was subsequently determined using a radioimmunoassay. We found that the estradiol synthesis was stimulated by ∼2-fold following a 48-h treatment with 0.1μM tributyltin, and this was accompanied by transcriptional activation of P450(17α) and P450arom. Tributyltin stimulated de novo hippocampal estradiol synthesis by modifying the transcription of specific steroidogenic enzymes.",
author = "Eiji Munetsuna and Minoru Hattori and Takeshi Yamazaki",
year = "2014",
month = "1",
day = "11",
doi = "10.3109/07435800.2013.875563",
language = "English",
volume = "39",
pages = "168--172",
journal = "Endocrine Research",
issn = "0743-5800",
publisher = "Informa Healthcare",
number = "4",

}

Stimulation of estradiol biosynthesis by tributyltin in rat hippocampal slices. / Munetsuna, Eiji; Hattori, Minoru; Yamazaki, Takeshi.

In: Endocrine Research, Vol. 39, No. 4, 11.01.2014, p. 168-172.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Stimulation of estradiol biosynthesis by tributyltin in rat hippocampal slices

AU - Munetsuna, Eiji

AU - Hattori, Minoru

AU - Yamazaki, Takeshi

PY - 2014/1/11

Y1 - 2014/1/11

N2 - Hippocampal functions are influenced by steroid hormones, such as testosterone and estradiol. It has been demonstrated that hippocampus-derived steroid hormones play important roles in neuronal protection and synapse formation. Our research groups have demonstrated that estradiol is de novo synthesized in the rat hippocampus. However, the mechanism(s) regulating this synthesis remains unclear. It has been reported that tributyltin, an environmental pollutant, binds to the retinoid X receptor (RXR) and modifies estrogen synthesis in human granulosa-like tumor cells. This compound can penetrate the blood brain barrier, and tends to accumulate in the brain. Based on these facts, we hypothesized that tributyltin could influence the hippocampal estradiol synthesis. A concentration of 0.1μM tributyltin induced an increase in the mRNA content of P450(17α) and P450arom in hippocampal slices, as determined using real-time PCR. The transcript levels of other steroidogenic enzymes and a steroidogenic acute regulatory protein were not affected. The estradiol level in rat hippocampal slices was subsequently determined using a radioimmunoassay. We found that the estradiol synthesis was stimulated by ∼2-fold following a 48-h treatment with 0.1μM tributyltin, and this was accompanied by transcriptional activation of P450(17α) and P450arom. Tributyltin stimulated de novo hippocampal estradiol synthesis by modifying the transcription of specific steroidogenic enzymes.

AB - Hippocampal functions are influenced by steroid hormones, such as testosterone and estradiol. It has been demonstrated that hippocampus-derived steroid hormones play important roles in neuronal protection and synapse formation. Our research groups have demonstrated that estradiol is de novo synthesized in the rat hippocampus. However, the mechanism(s) regulating this synthesis remains unclear. It has been reported that tributyltin, an environmental pollutant, binds to the retinoid X receptor (RXR) and modifies estrogen synthesis in human granulosa-like tumor cells. This compound can penetrate the blood brain barrier, and tends to accumulate in the brain. Based on these facts, we hypothesized that tributyltin could influence the hippocampal estradiol synthesis. A concentration of 0.1μM tributyltin induced an increase in the mRNA content of P450(17α) and P450arom in hippocampal slices, as determined using real-time PCR. The transcript levels of other steroidogenic enzymes and a steroidogenic acute regulatory protein were not affected. The estradiol level in rat hippocampal slices was subsequently determined using a radioimmunoassay. We found that the estradiol synthesis was stimulated by ∼2-fold following a 48-h treatment with 0.1μM tributyltin, and this was accompanied by transcriptional activation of P450(17α) and P450arom. Tributyltin stimulated de novo hippocampal estradiol synthesis by modifying the transcription of specific steroidogenic enzymes.

UR - http://www.scopus.com/inward/record.url?scp=84911465505&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84911465505&partnerID=8YFLogxK

U2 - 10.3109/07435800.2013.875563

DO - 10.3109/07435800.2013.875563

M3 - Article

C2 - 24679120

AN - SCOPUS:84911465505

VL - 39

SP - 168

EP - 172

JO - Endocrine Research

JF - Endocrine Research

SN - 0743-5800

IS - 4

ER -