Stimulation of Na-dependent phosphate transport by platelet-derived growth factor in rat aortic smooth muscle cells

Ayako Kakita, Atsushi Suzuki, Keiko Nishiwaki, Yasunaga Ono, Motoko Kotake, Yoh Ariyoshi, Yoshitaka Miura, Mitsuyasu Ltoh, Yutaka Oiso

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Abstract

We investigated the effect of platelet-derived growth factor B homodimer (PDGF-BB) on inorganic phosphate (Pi) transport activity, which has been reported to be involved in the mechanism of atherosclerosis, in A-10 rat aortic vascular smooth muscle cells (VSMCs). PDGF-BB time- and dose-dependently stimulated Pi transport in A-10 cells. Using northern blot analysis, the PDGF-BB-enhanced Pi transporter (PiT) in A-10 cells was identified as Pit-1 (Glvr-1), a member of the type III Na-dependent PiT. An inhibitor of PDGF β-receptor tyrosine kinase suppressed PDGF-BB-induced Pi transport. Both a protein kinase C (PKC) inhibitor calphostin C and PKC down regulation suppressed the stimulatory effect of PDGF-BB on Pi transport. On the other hand, inhibition of mitogen-activated protein (MAP) kinases by selective inhibitors did not affect Pi transport. Ly294002, a phosphatidylinositol (PI) 3-kinase inhibitor, partially attenuated PDGF-BB-induced Pi transport. A selective inhibitor of S6 kinase, rapamycin, reduced this effect of PDGF-BB, while Akt kinase inhibitor did not. In summary, these results indicated that PDGF-BB is a potent and selective stimulator of Pi transport in VSMCs. The mechanism responsible for this effect is not mediated by MAP kinase, but involves activation of PKC, PI 3-kinase and S6 kinase.

Original languageEnglish
Pages (from-to)17-24
Number of pages8
JournalAtherosclerosis
Volume174
Issue number1
DOIs
Publication statusPublished - 01-05-2004

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Platelet-Derived Growth Factor
Smooth Muscle Myocytes
Phosphates
antineoplaston A10
Phosphatidylinositol 3-Kinase
Protein Kinase C
Ribosomal Protein S6 Kinases
Protein Kinase Inhibitors
Mitogen-Activated Protein Kinases
Vascular Smooth Muscle
Proto-Oncogene Proteins c-sis
platelet-derived growth factor BB
Protein C Inhibitor
Sirolimus
Northern Blotting
Atherosclerosis
Phosphotransferases
Down-Regulation

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Kakita, Ayako ; Suzuki, Atsushi ; Nishiwaki, Keiko ; Ono, Yasunaga ; Kotake, Motoko ; Ariyoshi, Yoh ; Miura, Yoshitaka ; Ltoh, Mitsuyasu ; Oiso, Yutaka. / Stimulation of Na-dependent phosphate transport by platelet-derived growth factor in rat aortic smooth muscle cells. In: Atherosclerosis. 2004 ; Vol. 174, No. 1. pp. 17-24.
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Stimulation of Na-dependent phosphate transport by platelet-derived growth factor in rat aortic smooth muscle cells. / Kakita, Ayako; Suzuki, Atsushi; Nishiwaki, Keiko; Ono, Yasunaga; Kotake, Motoko; Ariyoshi, Yoh; Miura, Yoshitaka; Ltoh, Mitsuyasu; Oiso, Yutaka.

In: Atherosclerosis, Vol. 174, No. 1, 01.05.2004, p. 17-24.

Research output: Contribution to journalArticle

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T1 - Stimulation of Na-dependent phosphate transport by platelet-derived growth factor in rat aortic smooth muscle cells

AU - Kakita, Ayako

AU - Suzuki, Atsushi

AU - Nishiwaki, Keiko

AU - Ono, Yasunaga

AU - Kotake, Motoko

AU - Ariyoshi, Yoh

AU - Miura, Yoshitaka

AU - Ltoh, Mitsuyasu

AU - Oiso, Yutaka

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N2 - We investigated the effect of platelet-derived growth factor B homodimer (PDGF-BB) on inorganic phosphate (Pi) transport activity, which has been reported to be involved in the mechanism of atherosclerosis, in A-10 rat aortic vascular smooth muscle cells (VSMCs). PDGF-BB time- and dose-dependently stimulated Pi transport in A-10 cells. Using northern blot analysis, the PDGF-BB-enhanced Pi transporter (PiT) in A-10 cells was identified as Pit-1 (Glvr-1), a member of the type III Na-dependent PiT. An inhibitor of PDGF β-receptor tyrosine kinase suppressed PDGF-BB-induced Pi transport. Both a protein kinase C (PKC) inhibitor calphostin C and PKC down regulation suppressed the stimulatory effect of PDGF-BB on Pi transport. On the other hand, inhibition of mitogen-activated protein (MAP) kinases by selective inhibitors did not affect Pi transport. Ly294002, a phosphatidylinositol (PI) 3-kinase inhibitor, partially attenuated PDGF-BB-induced Pi transport. A selective inhibitor of S6 kinase, rapamycin, reduced this effect of PDGF-BB, while Akt kinase inhibitor did not. In summary, these results indicated that PDGF-BB is a potent and selective stimulator of Pi transport in VSMCs. The mechanism responsible for this effect is not mediated by MAP kinase, but involves activation of PKC, PI 3-kinase and S6 kinase.

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