Stimulation of TIMP-1 production by oncostatin M in human articular cartilage

Osamu Nemoto, Harumoto Yamada, Masahiro Mukaida, Masayuki Shimmei

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Objective. To investigate the effects of the interleukin-6 (IL-6) family cytokines, such as IL-6, IL-11, leukemia inhibitory factor (LIF), and oncostatin M (OSM), on the production of tissue inhibitor of matrix metalloproteinases 1 (TIMP-1) in human articular cartilage. Methods. Effects of IL-6 family cytokines and growth factors on TIMP-1 production were studied in human articular chondrocytes, grown as monolayer and cartilage explant culture. TIMP-1 protein levels in the cultured medium were measured by sandwich enzyme immunoassay. TIMP-1 messenger RNA levels in the cultured chondrocytes were analyzed by Northern blotting. Western blot analysis was performed to evaluate the release of matrix metalloproteinases (MMPs) in the cultured medium. Cell proliferation of chondrocytes was determined by 3H- thymidine uptake. Results. IL-6 family cytokines induced TIMP-1 expression in monolayer and explant culture, and the production of TIMP-1 was most stimulated by OSM. In contrast, OSM did not modulate the release of MMPs and cell proliferation. Conclusion. These results suggest that OSM may be characterized as one of the chondroprotective mediators in cartilage destruction.

Original languageEnglish
Pages (from-to)560-566
Number of pages7
JournalArthritis and Rheumatism
Volume39
Issue number4
DOIs
Publication statusPublished - 01-04-1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'Stimulation of TIMP-1 production by oncostatin M in human articular cartilage'. Together they form a unique fingerprint.

  • Cite this