TY - JOUR
T1 - Stimulatory effect of prostaglandin F2α on Na-dependent phosphate transport in osteoblast-like cells
AU - Sato, I.
AU - Suzuki, A.
AU - Kakita, A.
AU - Ono, Y.
AU - Miura, Y.
AU - Itoh, M.
AU - Oiso, Y.
PY - 2003/5/1
Y1 - 2003/5/1
N2 - Prostaglandin F2α (PGF2α) has been reported to activate protein kinase C (PKC) through both phospholipase (PL) C and D, resulting in the proliferation of osteoblast-like cells. In addition, it has also been reported that Erk mitogen-activated protein kinase is also involved in the mechanism of PGF2α-induced proliferation of these cells. Recently, we have reported that several growth factors stimulate Na-dependent phosphate transport (Pi transport) activity of osteoblast-like cells, which has been recognized to play an important role in their mineralization. In the present study, we investigated the effect of PGF2α on Pi transport in MC3T3-E1 osteoblast-like cells. PGF2α stimulated Na-dependent Pi transport dose dependently in the range between 1 nM and 10 μM in MC3T3-E1 cells. The effect was time dependent up to 24h. Kinetic analysis revealed that PGF2α induces newly synthesized Pi transporter. Pretreatment with actinomycin D and cycloheximide suppressed PGF2α-induced enhancement of Pi transport. Combined effect of PMA and PGF2α was not additive in Pi transport. Calphostin C, a PKC inhibitor, dose-dependently suppressed Pi transport induced by PGF2α. On the contrary, U0126, which inhibits an upstream kinase of Erk (MEK), did not affect PGF2α-induced enhancement of Pi transport. In conclusion, PGF2α stimulates Pi transport through activation of PKC in osteoblast-like cells.
AB - Prostaglandin F2α (PGF2α) has been reported to activate protein kinase C (PKC) through both phospholipase (PL) C and D, resulting in the proliferation of osteoblast-like cells. In addition, it has also been reported that Erk mitogen-activated protein kinase is also involved in the mechanism of PGF2α-induced proliferation of these cells. Recently, we have reported that several growth factors stimulate Na-dependent phosphate transport (Pi transport) activity of osteoblast-like cells, which has been recognized to play an important role in their mineralization. In the present study, we investigated the effect of PGF2α on Pi transport in MC3T3-E1 osteoblast-like cells. PGF2α stimulated Na-dependent Pi transport dose dependently in the range between 1 nM and 10 μM in MC3T3-E1 cells. The effect was time dependent up to 24h. Kinetic analysis revealed that PGF2α induces newly synthesized Pi transporter. Pretreatment with actinomycin D and cycloheximide suppressed PGF2α-induced enhancement of Pi transport. Combined effect of PMA and PGF2α was not additive in Pi transport. Calphostin C, a PKC inhibitor, dose-dependently suppressed Pi transport induced by PGF2α. On the contrary, U0126, which inhibits an upstream kinase of Erk (MEK), did not affect PGF2α-induced enhancement of Pi transport. In conclusion, PGF2α stimulates Pi transport through activation of PKC in osteoblast-like cells.
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U2 - 10.1016/S0952-3278(03)00021-8
DO - 10.1016/S0952-3278(03)00021-8
M3 - Article
C2 - 12711247
AN - SCOPUS:0038409076
SN - 0952-3278
VL - 68
SP - 311
EP - 315
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
IS - 5
ER -