Stimulatory effect of prostaglandin F on Na-dependent phosphate transport in osteoblast-like cells

I. Sato, A. Suzuki, A. Kakita, Y. Ono, Y. Miura, M. Itoh, Y. Oiso

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Prostaglandin F (PGF) has been reported to activate protein kinase C (PKC) through both phospholipase (PL) C and D, resulting in the proliferation of osteoblast-like cells. In addition, it has also been reported that Erk mitogen-activated protein kinase is also involved in the mechanism of PGF-induced proliferation of these cells. Recently, we have reported that several growth factors stimulate Na-dependent phosphate transport (Pi transport) activity of osteoblast-like cells, which has been recognized to play an important role in their mineralization. In the present study, we investigated the effect of PGF on Pi transport in MC3T3-E1 osteoblast-like cells. PGF stimulated Na-dependent Pi transport dose dependently in the range between 1 nM and 10 μM in MC3T3-E1 cells. The effect was time dependent up to 24h. Kinetic analysis revealed that PGF induces newly synthesized Pi transporter. Pretreatment with actinomycin D and cycloheximide suppressed PGF-induced enhancement of Pi transport. Combined effect of PMA and PGF was not additive in Pi transport. Calphostin C, a PKC inhibitor, dose-dependently suppressed Pi transport induced by PGF. On the contrary, U0126, which inhibits an upstream kinase of Erk (MEK), did not affect PGF-induced enhancement of Pi transport. In conclusion, PGF stimulates Pi transport through activation of PKC in osteoblast-like cells.

Original languageEnglish
Pages (from-to)311-315
Number of pages5
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume68
Issue number5
DOIs
Publication statusPublished - 01-05-2003

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Cell Biology

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