Stimulatory effect of prostaglandin F on Na-dependent phosphate transport in osteoblast-like cells

I. Sato, Atsushi Suzuki, A. Kakita, Y. Ono, Y. Miura, M. Itoh, Y. Oiso

Research output: Contribution to journalArticle

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Abstract

Prostaglandin F (PGF ) has been reported to activate protein kinase C (PKC) through both phospholipase (PL) C and D, resulting in the proliferation of osteoblast-like cells. In addition, it has also been reported that Erk mitogen-activated protein kinase is also involved in the mechanism of PGF -induced proliferation of these cells. Recently, we have reported that several growth factors stimulate Na-dependent phosphate transport (Pi transport) activity of osteoblast-like cells, which has been recognized to play an important role in their mineralization. In the present study, we investigated the effect of PGF on Pi transport in MC3T3-E1 osteoblast-like cells. PGF stimulated Na-dependent Pi transport dose dependently in the range between 1 nM and 10 μM in MC3T3-E1 cells. The effect was time dependent up to 24h. Kinetic analysis revealed that PGF induces newly synthesized Pi transporter. Pretreatment with actinomycin D and cycloheximide suppressed PGF -induced enhancement of Pi transport. Combined effect of PMA and PGF was not additive in Pi transport. Calphostin C, a PKC inhibitor, dose-dependently suppressed Pi transport induced by PGF . On the contrary, U0126, which inhibits an upstream kinase of Erk (MEK), did not affect PGF -induced enhancement of Pi transport. In conclusion, PGF stimulates Pi transport through activation of PKC in osteoblast-like cells.

Original languageEnglish
Pages (from-to)311-315
Number of pages5
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume68
Issue number5
DOIs
Publication statusPublished - 01-05-2003

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Osteoblasts
Prostaglandins F
Phosphates
Protein Kinase C
MAP Kinase Kinase Kinases
Phospholipase D
Protein C Inhibitor
Mitogen-Activated Protein Kinase Kinases
Dactinomycin
Type C Phospholipases
Cycloheximide
Protein Kinase Inhibitors
Mitogen-Activated Protein Kinases
Intercellular Signaling Peptides and Proteins
Phosphotransferases
Chemical activation
Cells
Cell Proliferation
Kinetics

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Cell Biology

Cite this

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title = "Stimulatory effect of prostaglandin F 2α on Na-dependent phosphate transport in osteoblast-like cells",
abstract = "Prostaglandin F 2α (PGF 2α ) has been reported to activate protein kinase C (PKC) through both phospholipase (PL) C and D, resulting in the proliferation of osteoblast-like cells. In addition, it has also been reported that Erk mitogen-activated protein kinase is also involved in the mechanism of PGF 2α -induced proliferation of these cells. Recently, we have reported that several growth factors stimulate Na-dependent phosphate transport (Pi transport) activity of osteoblast-like cells, which has been recognized to play an important role in their mineralization. In the present study, we investigated the effect of PGF 2α on Pi transport in MC3T3-E1 osteoblast-like cells. PGF 2α stimulated Na-dependent Pi transport dose dependently in the range between 1 nM and 10 μM in MC3T3-E1 cells. The effect was time dependent up to 24h. Kinetic analysis revealed that PGF 2α induces newly synthesized Pi transporter. Pretreatment with actinomycin D and cycloheximide suppressed PGF 2α -induced enhancement of Pi transport. Combined effect of PMA and PGF 2α was not additive in Pi transport. Calphostin C, a PKC inhibitor, dose-dependently suppressed Pi transport induced by PGF 2α . On the contrary, U0126, which inhibits an upstream kinase of Erk (MEK), did not affect PGF 2α -induced enhancement of Pi transport. In conclusion, PGF 2α stimulates Pi transport through activation of PKC in osteoblast-like cells.",
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Stimulatory effect of prostaglandin F on Na-dependent phosphate transport in osteoblast-like cells . / Sato, I.; Suzuki, Atsushi; Kakita, A.; Ono, Y.; Miura, Y.; Itoh, M.; Oiso, Y.

In: Prostaglandins Leukotrienes and Essential Fatty Acids, Vol. 68, No. 5, 01.05.2003, p. 311-315.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Stimulatory effect of prostaglandin F 2α on Na-dependent phosphate transport in osteoblast-like cells

AU - Sato, I.

AU - Suzuki, Atsushi

AU - Kakita, A.

AU - Ono, Y.

AU - Miura, Y.

AU - Itoh, M.

AU - Oiso, Y.

PY - 2003/5/1

Y1 - 2003/5/1

N2 - Prostaglandin F 2α (PGF 2α ) has been reported to activate protein kinase C (PKC) through both phospholipase (PL) C and D, resulting in the proliferation of osteoblast-like cells. In addition, it has also been reported that Erk mitogen-activated protein kinase is also involved in the mechanism of PGF 2α -induced proliferation of these cells. Recently, we have reported that several growth factors stimulate Na-dependent phosphate transport (Pi transport) activity of osteoblast-like cells, which has been recognized to play an important role in their mineralization. In the present study, we investigated the effect of PGF 2α on Pi transport in MC3T3-E1 osteoblast-like cells. PGF 2α stimulated Na-dependent Pi transport dose dependently in the range between 1 nM and 10 μM in MC3T3-E1 cells. The effect was time dependent up to 24h. Kinetic analysis revealed that PGF 2α induces newly synthesized Pi transporter. Pretreatment with actinomycin D and cycloheximide suppressed PGF 2α -induced enhancement of Pi transport. Combined effect of PMA and PGF 2α was not additive in Pi transport. Calphostin C, a PKC inhibitor, dose-dependently suppressed Pi transport induced by PGF 2α . On the contrary, U0126, which inhibits an upstream kinase of Erk (MEK), did not affect PGF 2α -induced enhancement of Pi transport. In conclusion, PGF 2α stimulates Pi transport through activation of PKC in osteoblast-like cells.

AB - Prostaglandin F 2α (PGF 2α ) has been reported to activate protein kinase C (PKC) through both phospholipase (PL) C and D, resulting in the proliferation of osteoblast-like cells. In addition, it has also been reported that Erk mitogen-activated protein kinase is also involved in the mechanism of PGF 2α -induced proliferation of these cells. Recently, we have reported that several growth factors stimulate Na-dependent phosphate transport (Pi transport) activity of osteoblast-like cells, which has been recognized to play an important role in their mineralization. In the present study, we investigated the effect of PGF 2α on Pi transport in MC3T3-E1 osteoblast-like cells. PGF 2α stimulated Na-dependent Pi transport dose dependently in the range between 1 nM and 10 μM in MC3T3-E1 cells. The effect was time dependent up to 24h. Kinetic analysis revealed that PGF 2α induces newly synthesized Pi transporter. Pretreatment with actinomycin D and cycloheximide suppressed PGF 2α -induced enhancement of Pi transport. Combined effect of PMA and PGF 2α was not additive in Pi transport. Calphostin C, a PKC inhibitor, dose-dependently suppressed Pi transport induced by PGF 2α . On the contrary, U0126, which inhibits an upstream kinase of Erk (MEK), did not affect PGF 2α -induced enhancement of Pi transport. In conclusion, PGF 2α stimulates Pi transport through activation of PKC in osteoblast-like cells.

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