Structural analysis of the rat homologue of CD1: Evidence for evolutionary conservation of the CD1D class and widespread transcription by rat cells

Shingo Ichimiya, Kokichi Kikuchi, Akihiro Matsuura

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The cDNA encoding the rat homologue of CD1 was isolated and the complete nucleotide sequence was determined. It contained an open reading frame of 1008 bp that was capable of encoding a polypeptide with 336 amino acids composed of hydrophobic leader and transmembrane sequences, three extracellular domains, and 5' and 3' untranslated sequences. Comparison of the amino acid sequence of rat CD1 with those of other species revealed that it showed the highest similarity to mouse CD1, which belongs to the CD1D class of the CD1 system and is distinct from the classic CD1 class including CD1a, CD1b, and CD1c expressed primarily on human thymocytes and some dendritic cells. Widespread transcription of rat CD1 was readily detected by Northern blot analysis in nonlymphoid organs, including the liver, kidney, and heart, as well as in lymphoid organs, including the thymus, lymph node, and spleen. Intestinal expression was also demonstrated by the more sensitive reverse transcription-PCR method. Immunoprecipitation with a rabbit anti-rat CD1 Ab showed that rat CD1 was expressed on the cell surface as a β2- microglobulin-associated heterodimer. Southern blot analysis of inbred rat strains suggested that rat CD1 shows limited polymorphism and that only one CD1 gene is detectable in the F344 rat genome. These results provide evidence for the conservation of CD1D class through mammalian evolution and an apparent lack of the classic CD1 class genes in rodents. Functional similarity of rodent CD1 is implied.

Original languageEnglish
Pages (from-to)1112-1123
Number of pages12
JournalJournal of Immunology
Volume153
Issue number3
Publication statusPublished - 01-08-1994
Externally publishedYes

Fingerprint

Rodentia
Inbred Strains Rats
Inbred F344 Rats
Thymocytes
Southern Blotting
Immunoprecipitation
Northern Blotting
Dendritic Cells
Thymus Gland
Open Reading Frames
Genes
Reverse Transcription
Amino Acid Sequence
Spleen
Complementary DNA
Lymph Nodes
Genome
Rabbits
Kidney
Amino Acids

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

@article{7d06516c933847e59df57e4e9d017df9,
title = "Structural analysis of the rat homologue of CD1: Evidence for evolutionary conservation of the CD1D class and widespread transcription by rat cells",
abstract = "The cDNA encoding the rat homologue of CD1 was isolated and the complete nucleotide sequence was determined. It contained an open reading frame of 1008 bp that was capable of encoding a polypeptide with 336 amino acids composed of hydrophobic leader and transmembrane sequences, three extracellular domains, and 5' and 3' untranslated sequences. Comparison of the amino acid sequence of rat CD1 with those of other species revealed that it showed the highest similarity to mouse CD1, which belongs to the CD1D class of the CD1 system and is distinct from the classic CD1 class including CD1a, CD1b, and CD1c expressed primarily on human thymocytes and some dendritic cells. Widespread transcription of rat CD1 was readily detected by Northern blot analysis in nonlymphoid organs, including the liver, kidney, and heart, as well as in lymphoid organs, including the thymus, lymph node, and spleen. Intestinal expression was also demonstrated by the more sensitive reverse transcription-PCR method. Immunoprecipitation with a rabbit anti-rat CD1 Ab showed that rat CD1 was expressed on the cell surface as a β2- microglobulin-associated heterodimer. Southern blot analysis of inbred rat strains suggested that rat CD1 shows limited polymorphism and that only one CD1 gene is detectable in the F344 rat genome. These results provide evidence for the conservation of CD1D class through mammalian evolution and an apparent lack of the classic CD1 class genes in rodents. Functional similarity of rodent CD1 is implied.",
author = "Shingo Ichimiya and Kokichi Kikuchi and Akihiro Matsuura",
year = "1994",
month = "8",
day = "1",
language = "English",
volume = "153",
pages = "1112--1123",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

}

Structural analysis of the rat homologue of CD1 : Evidence for evolutionary conservation of the CD1D class and widespread transcription by rat cells. / Ichimiya, Shingo; Kikuchi, Kokichi; Matsuura, Akihiro.

In: Journal of Immunology, Vol. 153, No. 3, 01.08.1994, p. 1112-1123.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Structural analysis of the rat homologue of CD1

T2 - Evidence for evolutionary conservation of the CD1D class and widespread transcription by rat cells

AU - Ichimiya, Shingo

AU - Kikuchi, Kokichi

AU - Matsuura, Akihiro

PY - 1994/8/1

Y1 - 1994/8/1

N2 - The cDNA encoding the rat homologue of CD1 was isolated and the complete nucleotide sequence was determined. It contained an open reading frame of 1008 bp that was capable of encoding a polypeptide with 336 amino acids composed of hydrophobic leader and transmembrane sequences, three extracellular domains, and 5' and 3' untranslated sequences. Comparison of the amino acid sequence of rat CD1 with those of other species revealed that it showed the highest similarity to mouse CD1, which belongs to the CD1D class of the CD1 system and is distinct from the classic CD1 class including CD1a, CD1b, and CD1c expressed primarily on human thymocytes and some dendritic cells. Widespread transcription of rat CD1 was readily detected by Northern blot analysis in nonlymphoid organs, including the liver, kidney, and heart, as well as in lymphoid organs, including the thymus, lymph node, and spleen. Intestinal expression was also demonstrated by the more sensitive reverse transcription-PCR method. Immunoprecipitation with a rabbit anti-rat CD1 Ab showed that rat CD1 was expressed on the cell surface as a β2- microglobulin-associated heterodimer. Southern blot analysis of inbred rat strains suggested that rat CD1 shows limited polymorphism and that only one CD1 gene is detectable in the F344 rat genome. These results provide evidence for the conservation of CD1D class through mammalian evolution and an apparent lack of the classic CD1 class genes in rodents. Functional similarity of rodent CD1 is implied.

AB - The cDNA encoding the rat homologue of CD1 was isolated and the complete nucleotide sequence was determined. It contained an open reading frame of 1008 bp that was capable of encoding a polypeptide with 336 amino acids composed of hydrophobic leader and transmembrane sequences, three extracellular domains, and 5' and 3' untranslated sequences. Comparison of the amino acid sequence of rat CD1 with those of other species revealed that it showed the highest similarity to mouse CD1, which belongs to the CD1D class of the CD1 system and is distinct from the classic CD1 class including CD1a, CD1b, and CD1c expressed primarily on human thymocytes and some dendritic cells. Widespread transcription of rat CD1 was readily detected by Northern blot analysis in nonlymphoid organs, including the liver, kidney, and heart, as well as in lymphoid organs, including the thymus, lymph node, and spleen. Intestinal expression was also demonstrated by the more sensitive reverse transcription-PCR method. Immunoprecipitation with a rabbit anti-rat CD1 Ab showed that rat CD1 was expressed on the cell surface as a β2- microglobulin-associated heterodimer. Southern blot analysis of inbred rat strains suggested that rat CD1 shows limited polymorphism and that only one CD1 gene is detectable in the F344 rat genome. These results provide evidence for the conservation of CD1D class through mammalian evolution and an apparent lack of the classic CD1 class genes in rodents. Functional similarity of rodent CD1 is implied.

UR - http://www.scopus.com/inward/record.url?scp=0028289547&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028289547&partnerID=8YFLogxK

M3 - Article

C2 - 7517972

AN - SCOPUS:0028289547

VL - 153

SP - 1112

EP - 1123

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 3

ER -