Structural characterization of monosialo-, disialo- and trisialo-gangliosides by negative ion AP-MALDI-QIT-TOF mass spectrometry with MS n switching

Emi Ito, Akio Tominaga, Hiroaki Waki, Kozo Miseki, Azusa Tomioka, Kazuki Nakajima, Kazuaki Kakehi, Minoru Suzuki, Naoyuki Taniguchi, Akemi Suzuki

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) is a quite convenient soft ionization for biomolecules, keeping analytes atmospheric conditions instead of high vacuum conditions. In this study, an AP-MALDI ion source has been coupled to a quadrupole ion trap time-of-flight (QIT-TOF) mass spectrometer, which is able to perform MS n analysis. We applied this system to the structural characterization of monosialogangliosides, GM1 (NeuAc) and GM2 (NeuAc), disialogangliosides, GD2 (NeuAc, NeuAc), GD1a (NeuAc, NeuAc) and GD1b (NeuAc, NeuAc) and trisialoganglioside GT1a (NeuAc, NeuAc, NeuAc). In this system, the negative ion mass spectra of MS, MS 2 and MS 3, a set of three mass spectra, were able to measure within 2 s per cycle. Thus, obtained results demonstrate that the negative ion mode MS, MS 2 and MS 3 spectra provided sufficient information for the determination of molecular weights, oligosaccharide sequences and ceramide structures, and indicate that the AP-MALDI-QIT-TOF mass spectrometry keeping analytes atmospheric conditions with MS n switching is quite useful and convenient for structural analyses of various types of sialic acid-containing GSLs, gangliosides.

Original languageEnglish
Pages (from-to)1315-1324
Number of pages10
JournalNeurochemical Research
Volume37
Issue number6
DOIs
Publication statusPublished - 01-06-2012

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Atmospheric Pressure
Gangliosides
Atmospheric pressure
Ionization
Mass spectrometry
Desorption
Mass Spectrometry
Lasers
Negative ions
Ion sources
Ions
Ceramides
Biomolecules
N-Acetylneuraminic Acid
Mass spectrometers
Oligosaccharides
Molecular weight
Vacuum
Molecular Weight
sialogangliosides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Ito, Emi ; Tominaga, Akio ; Waki, Hiroaki ; Miseki, Kozo ; Tomioka, Azusa ; Nakajima, Kazuki ; Kakehi, Kazuaki ; Suzuki, Minoru ; Taniguchi, Naoyuki ; Suzuki, Akemi. / Structural characterization of monosialo-, disialo- and trisialo-gangliosides by negative ion AP-MALDI-QIT-TOF mass spectrometry with MS n switching. In: Neurochemical Research. 2012 ; Vol. 37, No. 6. pp. 1315-1324.
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abstract = "The atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) is a quite convenient soft ionization for biomolecules, keeping analytes atmospheric conditions instead of high vacuum conditions. In this study, an AP-MALDI ion source has been coupled to a quadrupole ion trap time-of-flight (QIT-TOF) mass spectrometer, which is able to perform MS n analysis. We applied this system to the structural characterization of monosialogangliosides, GM1 (NeuAc) and GM2 (NeuAc), disialogangliosides, GD2 (NeuAc, NeuAc), GD1a (NeuAc, NeuAc) and GD1b (NeuAc, NeuAc) and trisialoganglioside GT1a (NeuAc, NeuAc, NeuAc). In this system, the negative ion mass spectra of MS, MS 2 and MS 3, a set of three mass spectra, were able to measure within 2 s per cycle. Thus, obtained results demonstrate that the negative ion mode MS, MS 2 and MS 3 spectra provided sufficient information for the determination of molecular weights, oligosaccharide sequences and ceramide structures, and indicate that the AP-MALDI-QIT-TOF mass spectrometry keeping analytes atmospheric conditions with MS n switching is quite useful and convenient for structural analyses of various types of sialic acid-containing GSLs, gangliosides.",
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Structural characterization of monosialo-, disialo- and trisialo-gangliosides by negative ion AP-MALDI-QIT-TOF mass spectrometry with MS n switching. / Ito, Emi; Tominaga, Akio; Waki, Hiroaki; Miseki, Kozo; Tomioka, Azusa; Nakajima, Kazuki; Kakehi, Kazuaki; Suzuki, Minoru; Taniguchi, Naoyuki; Suzuki, Akemi.

In: Neurochemical Research, Vol. 37, No. 6, 01.06.2012, p. 1315-1324.

Research output: Contribution to journalArticle

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T1 - Structural characterization of monosialo-, disialo- and trisialo-gangliosides by negative ion AP-MALDI-QIT-TOF mass spectrometry with MS n switching

AU - Ito, Emi

AU - Tominaga, Akio

AU - Waki, Hiroaki

AU - Miseki, Kozo

AU - Tomioka, Azusa

AU - Nakajima, Kazuki

AU - Kakehi, Kazuaki

AU - Suzuki, Minoru

AU - Taniguchi, Naoyuki

AU - Suzuki, Akemi

PY - 2012/6/1

Y1 - 2012/6/1

N2 - The atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) is a quite convenient soft ionization for biomolecules, keeping analytes atmospheric conditions instead of high vacuum conditions. In this study, an AP-MALDI ion source has been coupled to a quadrupole ion trap time-of-flight (QIT-TOF) mass spectrometer, which is able to perform MS n analysis. We applied this system to the structural characterization of monosialogangliosides, GM1 (NeuAc) and GM2 (NeuAc), disialogangliosides, GD2 (NeuAc, NeuAc), GD1a (NeuAc, NeuAc) and GD1b (NeuAc, NeuAc) and trisialoganglioside GT1a (NeuAc, NeuAc, NeuAc). In this system, the negative ion mass spectra of MS, MS 2 and MS 3, a set of three mass spectra, were able to measure within 2 s per cycle. Thus, obtained results demonstrate that the negative ion mode MS, MS 2 and MS 3 spectra provided sufficient information for the determination of molecular weights, oligosaccharide sequences and ceramide structures, and indicate that the AP-MALDI-QIT-TOF mass spectrometry keeping analytes atmospheric conditions with MS n switching is quite useful and convenient for structural analyses of various types of sialic acid-containing GSLs, gangliosides.

AB - The atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) is a quite convenient soft ionization for biomolecules, keeping analytes atmospheric conditions instead of high vacuum conditions. In this study, an AP-MALDI ion source has been coupled to a quadrupole ion trap time-of-flight (QIT-TOF) mass spectrometer, which is able to perform MS n analysis. We applied this system to the structural characterization of monosialogangliosides, GM1 (NeuAc) and GM2 (NeuAc), disialogangliosides, GD2 (NeuAc, NeuAc), GD1a (NeuAc, NeuAc) and GD1b (NeuAc, NeuAc) and trisialoganglioside GT1a (NeuAc, NeuAc, NeuAc). In this system, the negative ion mass spectra of MS, MS 2 and MS 3, a set of three mass spectra, were able to measure within 2 s per cycle. Thus, obtained results demonstrate that the negative ion mode MS, MS 2 and MS 3 spectra provided sufficient information for the determination of molecular weights, oligosaccharide sequences and ceramide structures, and indicate that the AP-MALDI-QIT-TOF mass spectrometry keeping analytes atmospheric conditions with MS n switching is quite useful and convenient for structural analyses of various types of sialic acid-containing GSLs, gangliosides.

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