Structural elucidation of a variety of GalNAc-containing N-linked oligosaccharides from human urinary kallidinogenase

N. Tomiya, J. Awaya, M. Kurono, H. Hanzawa, I. Shimada, Y. Arata, T. Yoshida, N. Takahashi

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Abstract

Fifteen different structures of terminal GalNAc-containing N-linked oligosaccharides from human urinary kallidinogenase have been identified. These N-linked oligosaccharides were mostly neutral, because sialic acid content was lower than 0.13 mol of sialic acid/mol of sugar chain, and sulfate was not detected. The oligosaccharides were released from pepsin- digested protein by glycoamidase A (from almond) digestion. The reducing ends of the oligosaccharide chains were aminated with a fluorescent reagent, 2- aminopyridine. The resulting mixture of pyridylamino derivatives of the oligosaccharides were separated by high performance liquid chromatography on an ODS-silica column, and 15 oligosaccharides were isolated. The structure of each oligosaccharide fraction was analyzed by two-dimensional sugar mapping, component sugar analysis, high resolution proton nuclear magnetic resonance and methylation analysis. It was found that each N-linked oligosaccharide associated with human urinary kallidinogenase contains unsubstituted GalNAc residues at the nonreducing terminal. These 15 oligosaccharides include 5 biantennary, 7 triantennary, and 3 tetraantennary oligosaccharides.

Original languageEnglish
Pages (from-to)113-126
Number of pages14
JournalJournal of Biological Chemistry
Volume268
Issue number1
Publication statusPublished - 01-01-1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Tomiya, N., Awaya, J., Kurono, M., Hanzawa, H., Shimada, I., Arata, Y., Yoshida, T., & Takahashi, N. (1993). Structural elucidation of a variety of GalNAc-containing N-linked oligosaccharides from human urinary kallidinogenase. Journal of Biological Chemistry, 268(1), 113-126.