Structure and function of the hemolymph node in rats.

K. Sakita, M. Fujino, T. Koshikawa, Naoki Omiya, M. Ohbayashi, J. Asai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Hemolymph nodes (HLs) are unique lymph nodes, in that their lymphatic sinuses contain numerous erythrocytes. In this study, we compared the internal structure and immunologic function of HLs with those of ordinary lymph nodes (OLs) and the spleen. Electron microscopy revealed erythrocytes passing through the walls of blood vessels in the intermediate sinus area (IMSA) of a HL between expanded endothelial cell junctions. However, no direct communication was found between lymphatic sinuses and blood vessels. Numerous carbon particles appeared in the IMSA of HLs on 5 days after intravenous carbon particle injection, while OLs lacked particle deposition. Immunohistochemical studies showed that lipopolysaccharide (LPS) reached the IMSA of HLs and extravasated into medullary cords 4 hours after intravenous LPS injection, resulting in the appearance of more IgM-stained lymphocytes in the IMSA of HLs than in that of OLs on day 5. The ability of organs to produce antibodies was determined by counting the number of plaque forming colonies after intravenous injection of sheep red blood cells (SRBC). The HLs antibody-producing ability was between that of OLs and the spleen. These results suggest that HLs possess functionally open blood vessels in the IMSA and their immunologic capability is between that of OLs and the spleen. These findings suggests that HLs are lymphoid organs that have characteristics between those of the OLs and the spleen, both ultrastructurally and functionally.

Original languageEnglish
Pages (from-to)129-137
Number of pages9
JournalNagoya Journal of Medical Science
Volume60
Issue number3-4
Publication statusPublished - 01-01-1997
Externally publishedYes

Fingerprint

Hemolymph
Spleen
Blood Vessels
Aptitude
Erythrocytes
Intravenous Injections
Lipopolysaccharides
Carbon
Lymph Nodes
Lymphatic Vessels
Intercellular Junctions
Antibodies
Immunoglobulin M
Sheep
Electron Microscopy
Endothelial Cells
Communication
Lymphocytes
Injections

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Sakita, K., Fujino, M., Koshikawa, T., Omiya, N., Ohbayashi, M., & Asai, J. (1997). Structure and function of the hemolymph node in rats. Nagoya Journal of Medical Science, 60(3-4), 129-137.
Sakita, K. ; Fujino, M. ; Koshikawa, T. ; Omiya, Naoki ; Ohbayashi, M. ; Asai, J. / Structure and function of the hemolymph node in rats. In: Nagoya Journal of Medical Science. 1997 ; Vol. 60, No. 3-4. pp. 129-137.
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Sakita, K, Fujino, M, Koshikawa, T, Omiya, N, Ohbayashi, M & Asai, J 1997, 'Structure and function of the hemolymph node in rats.', Nagoya Journal of Medical Science, vol. 60, no. 3-4, pp. 129-137.

Structure and function of the hemolymph node in rats. / Sakita, K.; Fujino, M.; Koshikawa, T.; Omiya, Naoki; Ohbayashi, M.; Asai, J.

In: Nagoya Journal of Medical Science, Vol. 60, No. 3-4, 01.01.1997, p. 129-137.

Research output: Contribution to journalArticle

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Sakita K, Fujino M, Koshikawa T, Omiya N, Ohbayashi M, Asai J. Structure and function of the hemolymph node in rats. Nagoya Journal of Medical Science. 1997 Jan 1;60(3-4):129-137.