Structure and function of the pancreas in the polycystic kidney rat

Lanjuan Yi, Satoru Naruse, Sonoko Furuya, Akiko Yamamoto, Miyuki Nakakuki, Shizuko Nagao, Daisuke Yoshihara, Shigeru B.H. Ko, Muxin Wei, Takaharu Kondo, Hiroshi Ishiguro

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

OBJECTIVES: Mutation in the Pkhd1 gene that encodes a ciliary protein, fibrocystin, causes multiple cysts in the kidneys and liver in the polycystic kidney (PCK) rat, a model for human autosomal recessive PCK disease. To clarify the role of primary cilia in the pancreatic duct, we examined the structure and function of the exocrine pancreas of PCK rats. METHODS: Pancreatic juice and bile were collected from anesthetized rats. Pancreatic ductal structure was analyzed by microdissection and immunohistchemistry. RESULTS: Histologically pancreatic acini were apparently normal, and no cysts were detected in the pancreas. Larger pancreatic ducts were irregularly dilated with enhanced expression of AQP1 in epithelial cells. The pancreatic duct of PCK rats exhibited significantly (P < 0.05) higher distensibility than that of wild-type (WT) rat at a physiological luminal pressure (3 cm H2O). Pancreatic fluid secretion stimulated with a physiological dose of secretin (0.03 nmol/kg per hour) in PCK rats was significantly smaller than that in WT, but the differences were not significant at higher doses. The amylase responses to carbamylcholine were not different between PCK and WT rats. CONCLUSIONS: These findings suggest that fibrocystin/primary cilia-dependent mechanisms may play a role in the regulation of pancreatic ductal structure and fluid secretion.

Original languageEnglish
Pages (from-to)1292-1298
Number of pages7
JournalPancreas
Volume41
Issue number8
DOIs
Publication statusPublished - 01-11-2012

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Polycystic Kidney Diseases
Pancreas
Pancreatic Ducts
Fluids and Secretions
Cilia
Cysts
Autosomal Recessive Polycystic Kidney
Pancreatic Juice
Exocrine Pancreas
Microdissection
Secretin
Carbachol
Amylases
Bile
Epithelial Cells
Kidney
Pressure
Mutation
Liver
Genes

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

Cite this

Yi, L., Naruse, S., Furuya, S., Yamamoto, A., Nakakuki, M., Nagao, S., ... Ishiguro, H. (2012). Structure and function of the pancreas in the polycystic kidney rat. Pancreas, 41(8), 1292-1298. https://doi.org/10.1097/MPA.0b013e31824c12f9
Yi, Lanjuan ; Naruse, Satoru ; Furuya, Sonoko ; Yamamoto, Akiko ; Nakakuki, Miyuki ; Nagao, Shizuko ; Yoshihara, Daisuke ; Ko, Shigeru B.H. ; Wei, Muxin ; Kondo, Takaharu ; Ishiguro, Hiroshi. / Structure and function of the pancreas in the polycystic kidney rat. In: Pancreas. 2012 ; Vol. 41, No. 8. pp. 1292-1298.
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Yi, L, Naruse, S, Furuya, S, Yamamoto, A, Nakakuki, M, Nagao, S, Yoshihara, D, Ko, SBH, Wei, M, Kondo, T & Ishiguro, H 2012, 'Structure and function of the pancreas in the polycystic kidney rat', Pancreas, vol. 41, no. 8, pp. 1292-1298. https://doi.org/10.1097/MPA.0b013e31824c12f9

Structure and function of the pancreas in the polycystic kidney rat. / Yi, Lanjuan; Naruse, Satoru; Furuya, Sonoko; Yamamoto, Akiko; Nakakuki, Miyuki; Nagao, Shizuko; Yoshihara, Daisuke; Ko, Shigeru B.H.; Wei, Muxin; Kondo, Takaharu; Ishiguro, Hiroshi.

In: Pancreas, Vol. 41, No. 8, 01.11.2012, p. 1292-1298.

Research output: Contribution to journalArticle

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T1 - Structure and function of the pancreas in the polycystic kidney rat

AU - Yi, Lanjuan

AU - Naruse, Satoru

AU - Furuya, Sonoko

AU - Yamamoto, Akiko

AU - Nakakuki, Miyuki

AU - Nagao, Shizuko

AU - Yoshihara, Daisuke

AU - Ko, Shigeru B.H.

AU - Wei, Muxin

AU - Kondo, Takaharu

AU - Ishiguro, Hiroshi

PY - 2012/11/1

Y1 - 2012/11/1

N2 - OBJECTIVES: Mutation in the Pkhd1 gene that encodes a ciliary protein, fibrocystin, causes multiple cysts in the kidneys and liver in the polycystic kidney (PCK) rat, a model for human autosomal recessive PCK disease. To clarify the role of primary cilia in the pancreatic duct, we examined the structure and function of the exocrine pancreas of PCK rats. METHODS: Pancreatic juice and bile were collected from anesthetized rats. Pancreatic ductal structure was analyzed by microdissection and immunohistchemistry. RESULTS: Histologically pancreatic acini were apparently normal, and no cysts were detected in the pancreas. Larger pancreatic ducts were irregularly dilated with enhanced expression of AQP1 in epithelial cells. The pancreatic duct of PCK rats exhibited significantly (P < 0.05) higher distensibility than that of wild-type (WT) rat at a physiological luminal pressure (3 cm H2O). Pancreatic fluid secretion stimulated with a physiological dose of secretin (0.03 nmol/kg per hour) in PCK rats was significantly smaller than that in WT, but the differences were not significant at higher doses. The amylase responses to carbamylcholine were not different between PCK and WT rats. CONCLUSIONS: These findings suggest that fibrocystin/primary cilia-dependent mechanisms may play a role in the regulation of pancreatic ductal structure and fluid secretion.

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Yi L, Naruse S, Furuya S, Yamamoto A, Nakakuki M, Nagao S et al. Structure and function of the pancreas in the polycystic kidney rat. Pancreas. 2012 Nov 1;41(8):1292-1298. https://doi.org/10.1097/MPA.0b013e31824c12f9