Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication

Eiji Abe, Kazuhiko Kuwahara, Mikoto Yoshida, Mikio Suzuki, Hidenori Terasaki, Yoshinobu Matsuo, Ei Ichi Takahashi, Nobuo Sakaguchi

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A 210 kDa protein named GANP is upregulated in germinal center (GC)-B cells in the spleen of antigen-immunized mouse. We studied a human ganp gene (hganp) encoding a putative polypeptide of 1980 amino acids. The carboxyl-terminal 721-amino-acid sequence of hGANP is identical to Map80, that is presumably generated by alternative splicing of hganp/Map80 gene. The genomic segment carrying hganp and Map80 genes was isolated, and the chromosomal location was determined on 21q22.3. Northern blot analysis with RNAs from various organs demonstrated a single band of 7 kb hganp mRNA, which suggests a preferential transcription of hganp gene from the hganp/Map80 locus. The hGANP expression was upregulated in GCs of the tonsil, as demonstrated by in-situ RNA hybridization and immunohistochemical analyses. The hGANP, with the domain (Map-box) capable of binding to MCM3 in B cells, might be involved in regulation of cell-cycle progression and DNA replication of GC-B cells. (C) 2000 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)219-227
Number of pages9
JournalGene
Volume255
Issue number2
DOIs
Publication statusPublished - 19-09-2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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