TY - JOUR
T1 - Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication
AU - Abe, Eiji
AU - Kuwahara, Kazuhiko
AU - Yoshida, Mikoto
AU - Suzuki, Mikio
AU - Terasaki, Hidenori
AU - Matsuo, Yoshinobu
AU - Takahashi, Ei Ichi
AU - Sakaguchi, Nobuo
N1 - Funding Information:
The authors thank Drs Taichi Ezaki and Kenjirou Matsuno (Department of Anatomy II, Kumamoto University School of Medicine) for helpful comments regarding immunohistochemical staining. We also appreciate Dr Eisaku Kondo (Second Department of Pathology, Okayama University Medical School) for his suggestion on the in-situ RNA hybridization method. This work was supported in part by a grant-in-aid from the Ministry of Education, Science, Sports, and Culture of Japan.
PY - 2000/9/19
Y1 - 2000/9/19
N2 - A 210 kDa protein named GANP is upregulated in germinal center (GC)-B cells in the spleen of antigen-immunized mouse. We studied a human ganp gene (hganp) encoding a putative polypeptide of 1980 amino acids. The carboxyl-terminal 721-amino-acid sequence of hGANP is identical to Map80, that is presumably generated by alternative splicing of hganp/Map80 gene. The genomic segment carrying hganp and Map80 genes was isolated, and the chromosomal location was determined on 21q22.3. Northern blot analysis with RNAs from various organs demonstrated a single band of 7 kb hganp mRNA, which suggests a preferential transcription of hganp gene from the hganp/Map80 locus. The hGANP expression was upregulated in GCs of the tonsil, as demonstrated by in-situ RNA hybridization and immunohistochemical analyses. The hGANP, with the domain (Map-box) capable of binding to MCM3 in B cells, might be involved in regulation of cell-cycle progression and DNA replication of GC-B cells. (C) 2000 Elsevier Science B.V. All rights reserved.
AB - A 210 kDa protein named GANP is upregulated in germinal center (GC)-B cells in the spleen of antigen-immunized mouse. We studied a human ganp gene (hganp) encoding a putative polypeptide of 1980 amino acids. The carboxyl-terminal 721-amino-acid sequence of hGANP is identical to Map80, that is presumably generated by alternative splicing of hganp/Map80 gene. The genomic segment carrying hganp and Map80 genes was isolated, and the chromosomal location was determined on 21q22.3. Northern blot analysis with RNAs from various organs demonstrated a single band of 7 kb hganp mRNA, which suggests a preferential transcription of hganp gene from the hganp/Map80 locus. The hGANP expression was upregulated in GCs of the tonsil, as demonstrated by in-situ RNA hybridization and immunohistochemical analyses. The hGANP, with the domain (Map-box) capable of binding to MCM3 in B cells, might be involved in regulation of cell-cycle progression and DNA replication of GC-B cells. (C) 2000 Elsevier Science B.V. All rights reserved.
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U2 - 10.1016/S0378-1119(00)00336-X
DO - 10.1016/S0378-1119(00)00336-X
M3 - Article
C2 - 11024281
AN - SCOPUS:0034687059
VL - 255
SP - 219
EP - 227
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -