TY - JOUR
T1 - Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication
AU - Abe, Eiji
AU - Kuwahara, Kazuhiko
AU - Yoshida, Mikoto
AU - Suzuki, Mikio
AU - Terasaki, Hidenori
AU - Matsuo, Yoshinobu
AU - Takahashi, Ei Ichi
AU - Sakaguchi, Nobuo
PY - 2000/9/19
Y1 - 2000/9/19
N2 - A 210 kDa protein named GANP is upregulated in germinal center (GC)-B cells in the spleen of antigen-immunized mouse. We studied a human ganp gene (hganp) encoding a putative polypeptide of 1980 amino acids. The carboxyl-terminal 721-amino-acid sequence of hGANP is identical to Map80, that is presumably generated by alternative splicing of hganp/Map80 gene. The genomic segment carrying hganp and Map80 genes was isolated, and the chromosomal location was determined on 21q22.3. Northern blot analysis with RNAs from various organs demonstrated a single band of 7 kb hganp mRNA, which suggests a preferential transcription of hganp gene from the hganp/Map80 locus. The hGANP expression was upregulated in GCs of the tonsil, as demonstrated by in-situ RNA hybridization and immunohistochemical analyses. The hGANP, with the domain (Map-box) capable of binding to MCM3 in B cells, might be involved in regulation of cell-cycle progression and DNA replication of GC-B cells. (C) 2000 Elsevier Science B.V. All rights reserved.
AB - A 210 kDa protein named GANP is upregulated in germinal center (GC)-B cells in the spleen of antigen-immunized mouse. We studied a human ganp gene (hganp) encoding a putative polypeptide of 1980 amino acids. The carboxyl-terminal 721-amino-acid sequence of hGANP is identical to Map80, that is presumably generated by alternative splicing of hganp/Map80 gene. The genomic segment carrying hganp and Map80 genes was isolated, and the chromosomal location was determined on 21q22.3. Northern blot analysis with RNAs from various organs demonstrated a single band of 7 kb hganp mRNA, which suggests a preferential transcription of hganp gene from the hganp/Map80 locus. The hGANP expression was upregulated in GCs of the tonsil, as demonstrated by in-situ RNA hybridization and immunohistochemical analyses. The hGANP, with the domain (Map-box) capable of binding to MCM3 in B cells, might be involved in regulation of cell-cycle progression and DNA replication of GC-B cells. (C) 2000 Elsevier Science B.V. All rights reserved.
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U2 - 10.1016/S0378-1119(00)00336-X
DO - 10.1016/S0378-1119(00)00336-X
M3 - Article
C2 - 11024281
AN - SCOPUS:0034687059
VL - 255
SP - 219
EP - 227
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -