Structure of the gene encoding the mouse 47-kDa heat-shock protein (HSP47)

Hosokawa Nobuko, Hajime Takechi, Yokota Shinichi, Hirayoshi Kazunori, Nagata Kazuhiro

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

HSP47, a 47-kDa heat-shock protein (HSP), is a member of a group of HSPs with the unique characteristics of collagen binding as well as transformation sensitivity. The protein belongs to the serpin (serine protease inhibitor) superfamily as determined from its amino acid sequence homology. We have isolated and characterized the mouse HSP47 including about 1 kb of the 5'-flanking region. This gene spans about 7.8 kb, consisting of six exons separated by five introns. This exon-intron structure is different from other serpin family proteins. Southern blot analysis revealed the existence of a single copy of HSP47. The promoter region contains a TATA box, four Sp1-binding sites and one AP-1-binding site. A complete heat-shock element (HSE) was found between nucleotides (nt) -61 and -79. Furthermore, the heat inducibility was reproduced by transfecting mouse BALB/3T3 cells with a plasmid carrying cat under the control of the HSE-containing fragment (nt -197 and + 38) of HSP47. Computer analysis of the promoter region did not show marked homology to other vertebrate promoters.

Original languageEnglish
Pages (from-to)187-193
Number of pages7
JournalGene
Volume126
Issue number2
DOIs
Publication statusPublished - 30-04-1993

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Serine Proteinase Inhibitors
Hot Temperature
Genetic Promoter Regions
Introns
Exons
Shock
HSP47 Heat-Shock Proteins
Nucleotides
Binding Sites
BALB 3T3 Cells
Genes
Amino Acid Sequence Homology
TATA Box
5' Flanking Region
Transcription Factor AP-1
Southern Blotting
Vertebrates
Proteins
Cats
Plasmids

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Nobuko, Hosokawa ; Takechi, Hajime ; Shinichi, Yokota ; Kazunori, Hirayoshi ; Kazuhiro, Nagata. / Structure of the gene encoding the mouse 47-kDa heat-shock protein (HSP47). In: Gene. 1993 ; Vol. 126, No. 2. pp. 187-193.
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Nobuko, H, Takechi, H, Shinichi, Y, Kazunori, H & Kazuhiro, N 1993, 'Structure of the gene encoding the mouse 47-kDa heat-shock protein (HSP47)', Gene, vol. 126, no. 2, pp. 187-193. https://doi.org/10.1016/0378-1119(93)90366-B

Structure of the gene encoding the mouse 47-kDa heat-shock protein (HSP47). / Nobuko, Hosokawa; Takechi, Hajime; Shinichi, Yokota; Kazunori, Hirayoshi; Kazuhiro, Nagata.

In: Gene, Vol. 126, No. 2, 30.04.1993, p. 187-193.

Research output: Contribution to journalArticle

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T1 - Structure of the gene encoding the mouse 47-kDa heat-shock protein (HSP47)

AU - Nobuko, Hosokawa

AU - Takechi, Hajime

AU - Shinichi, Yokota

AU - Kazunori, Hirayoshi

AU - Kazuhiro, Nagata

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