TY - JOUR
T1 - Structure of the mouse tyrosine hydroxylase gene
AU - Iwata, Nakao
AU - Kobayashi, Kazuto
AU - Sasaoka, Toshikuni
AU - Hidaka, Hiroyoshi
AU - Nagatsu, Toshiharu
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research, Ministry of Education, Science and Culture, Japan. We would like to thank Drs. M. Katsuki and M. Kimura of Tokai University for helpful discussion and encouragement. We also thank Dr. Ichikawa of Nagoya University for valuable technical instruction.
PY - 1992/1/15
Y1 - 1992/1/15
N2 - The mouse tyrosine hydroxylase (TH) gene was isolated from a genomic library by cross-hybridization with human TH cDNA probe. Nucleotide sequence analysis of two overlapping genomic clones showed that this gene is split into 13 exons distributed about 7.5 kb in length. The transcription initiation site was determined by primer extension analysis with mouse adrenal gland poly(A)+RNA. The structure of the mouse TH gene was similar to that of the human TH gene, but it contained neither the alternative splice donor site around the 3′-end of the first exon nor an independent exon corresponding to the second exon of the human TH gene. There were the canonical TATA and GC boxes, cyclic AMP responsive element (CRE), and AP1 binding site in the 5′-flanking region of the mouse TH gene.
AB - The mouse tyrosine hydroxylase (TH) gene was isolated from a genomic library by cross-hybridization with human TH cDNA probe. Nucleotide sequence analysis of two overlapping genomic clones showed that this gene is split into 13 exons distributed about 7.5 kb in length. The transcription initiation site was determined by primer extension analysis with mouse adrenal gland poly(A)+RNA. The structure of the mouse TH gene was similar to that of the human TH gene, but it contained neither the alternative splice donor site around the 3′-end of the first exon nor an independent exon corresponding to the second exon of the human TH gene. There were the canonical TATA and GC boxes, cyclic AMP responsive element (CRE), and AP1 binding site in the 5′-flanking region of the mouse TH gene.
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U2 - 10.1016/S0006-291X(05)80151-2
DO - 10.1016/S0006-291X(05)80151-2
M3 - Article
C2 - 1346248
AN - SCOPUS:0026584718
VL - 182
SP - 348
EP - 354
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -