Studies on tissue and cellular distribution of indoleamine 2,3-dioxygenase 2: The absence of IDO1 upregulates IDO2 expression in the epididymis

Masakazu Fukunaga, Yasuko Yamamoto, Misaki Kawasoe, Yuko Arioka, Yuki Murakami, Masato Hoshi, Kuniaki Saito

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Indoleamine 2,3-dioxygenase 1 (IDO1) catabolizes tryptophan to kynurenine at the first step of tryptophan metabolism. Recently, in addition to IDO1, a new isoform called IDO2 was identified. In this study, we examined the tissue expression pattern of IDO2 mRNA and the cellular localization of expressed IDO2 protein in mice. IDO1 mRNA expression was observed in the colon and epididymis, whereas IDO2 mRNA expression was found in the cerebral cortex, liver, kidney, and epididymis. Immunohistochemical analysis revealed that IDO2 protein was exclusively expressed on the hepatocytes, interlobular bile ducts, neuronal cells of the cerebrum cortex, Purkinje cells of the cerebellum cortex, lamina epithelialis, proximal convoluted tubule, and the collecting tubule of the kidney. In the epididymis, IDO1 protein expression was restricted to the caput, whereas IDO2 protein expression was observed on the caput, corpus, and cauda. Both IDO proteins were expressed on the caput, but both showed a different protein expression pattern in the segments. Immunohistochemical analysis in IDO1-/- mouse epididymis showed that IDO2 protein was extensively upregulated due to the loss of IDO1 expression.

Original languageEnglish
Pages (from-to)854-860
Number of pages7
JournalJournal of Histochemistry and Cytochemistry
Volume60
Issue number11
DOIs
Publication statusPublished - 01-12-2012

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Indoleamine-Pyrrole 2,3,-Dioxygenase
Epididymis
Tissue Distribution
Up-Regulation
Proteins
Tryptophan
Messenger RNA
Collecting Kidney Tubules
Kynurenine
Purkinje Cells
Cerebrum
Bile Ducts
Cerebral Cortex
Cerebellum
Hepatocytes
Protein Isoforms
Colon
Kidney
Liver

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology
  • Medicine(all)

Cite this

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abstract = "Indoleamine 2,3-dioxygenase 1 (IDO1) catabolizes tryptophan to kynurenine at the first step of tryptophan metabolism. Recently, in addition to IDO1, a new isoform called IDO2 was identified. In this study, we examined the tissue expression pattern of IDO2 mRNA and the cellular localization of expressed IDO2 protein in mice. IDO1 mRNA expression was observed in the colon and epididymis, whereas IDO2 mRNA expression was found in the cerebral cortex, liver, kidney, and epididymis. Immunohistochemical analysis revealed that IDO2 protein was exclusively expressed on the hepatocytes, interlobular bile ducts, neuronal cells of the cerebrum cortex, Purkinje cells of the cerebellum cortex, lamina epithelialis, proximal convoluted tubule, and the collecting tubule of the kidney. In the epididymis, IDO1 protein expression was restricted to the caput, whereas IDO2 protein expression was observed on the caput, corpus, and cauda. Both IDO proteins were expressed on the caput, but both showed a different protein expression pattern in the segments. Immunohistochemical analysis in IDO1-/- mouse epididymis showed that IDO2 protein was extensively upregulated due to the loss of IDO1 expression.",
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Studies on tissue and cellular distribution of indoleamine 2,3-dioxygenase 2 : The absence of IDO1 upregulates IDO2 expression in the epididymis. / Fukunaga, Masakazu; Yamamoto, Yasuko; Kawasoe, Misaki; Arioka, Yuko; Murakami, Yuki; Hoshi, Masato; Saito, Kuniaki.

In: Journal of Histochemistry and Cytochemistry, Vol. 60, No. 11, 01.12.2012, p. 854-860.

Research output: Contribution to journalArticle

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AU - Fukunaga, Masakazu

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AU - Kawasoe, Misaki

AU - Arioka, Yuko

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AU - Hoshi, Masato

AU - Saito, Kuniaki

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