Study of transforming growth factor-β1 gene, mRNA, and protein in Japanese renal transplant recipients

K. Saigo, N. Akutsu, M. Maruyama, K. Otsuki, M. Hasegawa, H. Aoyama, I. Matsumoto, T. Asano, Takashi Kenmochi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background Transforming growth factor (TGF)-β1 may contribute to chronic allograft nephropathy and graft loss; however, the exact molecular mechanism remains unclear. Therefore, we assess the relationship between TGF-β1 gene polymorphisms, expression, and development of allograft nephropathy. Methods We studied 135 renal transplant recipients at our hospital. TGF-β1 gene polymorphisms (codons 10 and 25) were determined from peripheral blood leukocyte DNA. Plasma TGF-β1 mRNA was measured by real-time polymerase chain reaction and TGF-β1 protein levels were assessed by enzyme-linked immunosorbent assay. The relationship between TGF-β1 genotyping, expression, and rejection and results of renal biopsy were evaluated. Results The genotype frequency of transplant recipients was 49.6%, 30.4%, and 20.0% for C/T, C/C and T/T at codon 10, 100% for G/G at codon 25, respectively. According to the criteria of Banff '97 classification, 24 cases were classified as acute rejection and whose genotypes were 16, 3, and 5 cases for C/T, C/C and T/T at codon 10. Plasma mRNA expression was elevated in 14 cases and decreased in 8 cases after acute rejection. We measured 267 specimens of TGF-β1 protein and there was no relation between amount of TGF-β1 protein and mRNA. Conclusion Our results suggest that the relationship between plasma TGF-β1 expression and the development of allograft nephropathy remains uncertain. Frequency of allograft rejection differ with TGF-β1 codon 10 genotypes and the high-risk genotype was different from the reports of other countries.

Original languageEnglish
Pages (from-to)372-375
Number of pages4
JournalTransplantation Proceedings
Volume46
Issue number2
DOIs
Publication statusPublished - 01-01-2014

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Transforming Growth Factors
Kidney
Messenger RNA
Codon
Proteins
Allografts
Genotype
Transplant Recipients
Real-Time Polymerase Chain Reaction
Leukocytes
Enzyme-Linked Immunosorbent Assay
Transplants
Biopsy
Gene Expression
DNA

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

Cite this

Saigo, K. ; Akutsu, N. ; Maruyama, M. ; Otsuki, K. ; Hasegawa, M. ; Aoyama, H. ; Matsumoto, I. ; Asano, T. ; Kenmochi, Takashi. / Study of transforming growth factor-β1 gene, mRNA, and protein in Japanese renal transplant recipients. In: Transplantation Proceedings. 2014 ; Vol. 46, No. 2. pp. 372-375.
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abstract = "Background Transforming growth factor (TGF)-β1 may contribute to chronic allograft nephropathy and graft loss; however, the exact molecular mechanism remains unclear. Therefore, we assess the relationship between TGF-β1 gene polymorphisms, expression, and development of allograft nephropathy. Methods We studied 135 renal transplant recipients at our hospital. TGF-β1 gene polymorphisms (codons 10 and 25) were determined from peripheral blood leukocyte DNA. Plasma TGF-β1 mRNA was measured by real-time polymerase chain reaction and TGF-β1 protein levels were assessed by enzyme-linked immunosorbent assay. The relationship between TGF-β1 genotyping, expression, and rejection and results of renal biopsy were evaluated. Results The genotype frequency of transplant recipients was 49.6{\%}, 30.4{\%}, and 20.0{\%} for C/T, C/C and T/T at codon 10, 100{\%} for G/G at codon 25, respectively. According to the criteria of Banff '97 classification, 24 cases were classified as acute rejection and whose genotypes were 16, 3, and 5 cases for C/T, C/C and T/T at codon 10. Plasma mRNA expression was elevated in 14 cases and decreased in 8 cases after acute rejection. We measured 267 specimens of TGF-β1 protein and there was no relation between amount of TGF-β1 protein and mRNA. Conclusion Our results suggest that the relationship between plasma TGF-β1 expression and the development of allograft nephropathy remains uncertain. Frequency of allograft rejection differ with TGF-β1 codon 10 genotypes and the high-risk genotype was different from the reports of other countries.",
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Saigo, K, Akutsu, N, Maruyama, M, Otsuki, K, Hasegawa, M, Aoyama, H, Matsumoto, I, Asano, T & Kenmochi, T 2014, 'Study of transforming growth factor-β1 gene, mRNA, and protein in Japanese renal transplant recipients', Transplantation Proceedings, vol. 46, no. 2, pp. 372-375. https://doi.org/10.1016/j.transproceed.2013.11.139

Study of transforming growth factor-β1 gene, mRNA, and protein in Japanese renal transplant recipients. / Saigo, K.; Akutsu, N.; Maruyama, M.; Otsuki, K.; Hasegawa, M.; Aoyama, H.; Matsumoto, I.; Asano, T.; Kenmochi, Takashi.

In: Transplantation Proceedings, Vol. 46, No. 2, 01.01.2014, p. 372-375.

Research output: Contribution to journalArticle

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T1 - Study of transforming growth factor-β1 gene, mRNA, and protein in Japanese renal transplant recipients

AU - Saigo, K.

AU - Akutsu, N.

AU - Maruyama, M.

AU - Otsuki, K.

AU - Hasegawa, M.

AU - Aoyama, H.

AU - Matsumoto, I.

AU - Asano, T.

AU - Kenmochi, Takashi

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N2 - Background Transforming growth factor (TGF)-β1 may contribute to chronic allograft nephropathy and graft loss; however, the exact molecular mechanism remains unclear. Therefore, we assess the relationship between TGF-β1 gene polymorphisms, expression, and development of allograft nephropathy. Methods We studied 135 renal transplant recipients at our hospital. TGF-β1 gene polymorphisms (codons 10 and 25) were determined from peripheral blood leukocyte DNA. Plasma TGF-β1 mRNA was measured by real-time polymerase chain reaction and TGF-β1 protein levels were assessed by enzyme-linked immunosorbent assay. The relationship between TGF-β1 genotyping, expression, and rejection and results of renal biopsy were evaluated. Results The genotype frequency of transplant recipients was 49.6%, 30.4%, and 20.0% for C/T, C/C and T/T at codon 10, 100% for G/G at codon 25, respectively. According to the criteria of Banff '97 classification, 24 cases were classified as acute rejection and whose genotypes were 16, 3, and 5 cases for C/T, C/C and T/T at codon 10. Plasma mRNA expression was elevated in 14 cases and decreased in 8 cases after acute rejection. We measured 267 specimens of TGF-β1 protein and there was no relation between amount of TGF-β1 protein and mRNA. Conclusion Our results suggest that the relationship between plasma TGF-β1 expression and the development of allograft nephropathy remains uncertain. Frequency of allograft rejection differ with TGF-β1 codon 10 genotypes and the high-risk genotype was different from the reports of other countries.

AB - Background Transforming growth factor (TGF)-β1 may contribute to chronic allograft nephropathy and graft loss; however, the exact molecular mechanism remains unclear. Therefore, we assess the relationship between TGF-β1 gene polymorphisms, expression, and development of allograft nephropathy. Methods We studied 135 renal transplant recipients at our hospital. TGF-β1 gene polymorphisms (codons 10 and 25) were determined from peripheral blood leukocyte DNA. Plasma TGF-β1 mRNA was measured by real-time polymerase chain reaction and TGF-β1 protein levels were assessed by enzyme-linked immunosorbent assay. The relationship between TGF-β1 genotyping, expression, and rejection and results of renal biopsy were evaluated. Results The genotype frequency of transplant recipients was 49.6%, 30.4%, and 20.0% for C/T, C/C and T/T at codon 10, 100% for G/G at codon 25, respectively. According to the criteria of Banff '97 classification, 24 cases were classified as acute rejection and whose genotypes were 16, 3, and 5 cases for C/T, C/C and T/T at codon 10. Plasma mRNA expression was elevated in 14 cases and decreased in 8 cases after acute rejection. We measured 267 specimens of TGF-β1 protein and there was no relation between amount of TGF-β1 protein and mRNA. Conclusion Our results suggest that the relationship between plasma TGF-β1 expression and the development of allograft nephropathy remains uncertain. Frequency of allograft rejection differ with TGF-β1 codon 10 genotypes and the high-risk genotype was different from the reports of other countries.

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