Study on Immunological Function of Melanosis Faciei Feminae “Problems with examination of HLA antigens

Nineteen patients with melanosis faciei feminae (MFF), of whom thirteen with patch test positive reaction to Sudan I and the rests with negative reaction to the allergen were studied compared with thirty control subjects on the immunological function tests. Sudan I positive group and whole patients group had statistically significant strong reactions to DNCB sensitization test compared with the controls (P<0.01). Sudan I positive group showed statistically significant strong reactions to PHA intracutaneous test compared with the control group (P<0.05). HLA antigens were first studied with seventeen patients by Kitasato Biochemical Laboratories. The result was that HLA-B7 antigen had the frequency of 52. which deviated significantly from the control subjects with the relative risk of 10. 48 (P<0.0000997). Further studies on HLA antigens performed by Tokyo Clinical Laboratory made it clear HLA-B7 antigen was not an associated antigen with MFF and that there exist some differences in the results of examination of HLA antigens between two laboratories.