Subcutaneous tissue distribution of vancomycin from a fibrin glue/Dacron graft carrier

Katsuhiro Fujimoto, Keiko Yamamura, Takashi Osada, Tetsuo Hayashi, Toshitaka Nabeshima, Masahiro Matsushita, Naomichi Nishikimi, Tsunehisa Sakurai, Yuji Nimura

Research output: Contribution to journalArticle

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Abstract

We investigated the tissue distribution of vancomycin (VCM) incorporated in fibrin glue (FG) in a rat model. One VCM-loaded FG Dacron graft (VCM-FG, VCM 0.6 mg/graft) was implanted in the subcutaneous tissue of the anterior abdominal wall of each rat. VCM was injected intravenously at an equal dose (0.6 mg/rat) after implantation of one control graft (without VCM-FG). After the implantation and the iv injection of an equal dose of VCM (0.6 mg/rat), the tissue distribution of VCM for up to 24 h was determined through analysis of the implanted VCM-FG grafts, which releasd VCM over a 24 h period. The area under the VCM concentration-time curve (AUC) of the tissue was 89.58 μg · h/g after the implantation of the VCM-FG graft, and 7.40 μg · h/g after the iv injection of VCM, respectively. The targeting index of the tissue, defined as the ratio of AUC after the implantation of the VCM-FG graft to that after VCM iv injection, was 12.11. None of the six VCM-FG Dacron grafts after implantation became infected following inoculation with S. aureus ATCC 25923 (0.1 mL 108 CFU/mL). These results suggest that this VCM-FG Dacron graft delivery may be useful in preventing local infection by enhancing the delivery of VCM to the local areas of the implanted site in rats.

Original languageEnglish
Pages (from-to)564-567
Number of pages4
JournalJournal of Biomedical Materials Research
Volume36
Issue number4
DOIs
Publication statusPublished - 15-09-1997

Fingerprint

Fibrin Tissue Adhesive
Polyethylene Terephthalates
Glues
Vancomycin
Grafts
Tissue
Rats

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

Fujimoto, Katsuhiro ; Yamamura, Keiko ; Osada, Takashi ; Hayashi, Tetsuo ; Nabeshima, Toshitaka ; Matsushita, Masahiro ; Nishikimi, Naomichi ; Sakurai, Tsunehisa ; Nimura, Yuji. / Subcutaneous tissue distribution of vancomycin from a fibrin glue/Dacron graft carrier. In: Journal of Biomedical Materials Research. 1997 ; Vol. 36, No. 4. pp. 564-567.
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Subcutaneous tissue distribution of vancomycin from a fibrin glue/Dacron graft carrier. / Fujimoto, Katsuhiro; Yamamura, Keiko; Osada, Takashi; Hayashi, Tetsuo; Nabeshima, Toshitaka; Matsushita, Masahiro; Nishikimi, Naomichi; Sakurai, Tsunehisa; Nimura, Yuji.

In: Journal of Biomedical Materials Research, Vol. 36, No. 4, 15.09.1997, p. 564-567.

Research output: Contribution to journalArticle

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AU - Fujimoto, Katsuhiro

AU - Yamamura, Keiko

AU - Osada, Takashi

AU - Hayashi, Tetsuo

AU - Nabeshima, Toshitaka

AU - Matsushita, Masahiro

AU - Nishikimi, Naomichi

AU - Sakurai, Tsunehisa

AU - Nimura, Yuji

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N2 - We investigated the tissue distribution of vancomycin (VCM) incorporated in fibrin glue (FG) in a rat model. One VCM-loaded FG Dacron graft (VCM-FG, VCM 0.6 mg/graft) was implanted in the subcutaneous tissue of the anterior abdominal wall of each rat. VCM was injected intravenously at an equal dose (0.6 mg/rat) after implantation of one control graft (without VCM-FG). After the implantation and the iv injection of an equal dose of VCM (0.6 mg/rat), the tissue distribution of VCM for up to 24 h was determined through analysis of the implanted VCM-FG grafts, which releasd VCM over a 24 h period. The area under the VCM concentration-time curve (AUC) of the tissue was 89.58 μg · h/g after the implantation of the VCM-FG graft, and 7.40 μg · h/g after the iv injection of VCM, respectively. The targeting index of the tissue, defined as the ratio of AUC after the implantation of the VCM-FG graft to that after VCM iv injection, was 12.11. None of the six VCM-FG Dacron grafts after implantation became infected following inoculation with S. aureus ATCC 25923 (0.1 mL 108 CFU/mL). These results suggest that this VCM-FG Dacron graft delivery may be useful in preventing local infection by enhancing the delivery of VCM to the local areas of the implanted site in rats.

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