TY - JOUR
T1 - Successful transplantation of rat hearts subjected to extended cold preservation with a novel preservation solution
AU - Wakayama, Kenji
AU - Fukai, Moto
AU - Yamashita, Kenichiro
AU - Kimura, Taichi
AU - Hirokata, Gentaro
AU - Shibasaki, Susumu
AU - Fukumori, Daisuke
AU - Haga, Sanae
AU - Sugawara, Mitsuru
AU - Suzuki, Tomomi
AU - Taniguchi, Masahiko
AU - Shimamura, Tsuyoshi
AU - Furukawa, Hiroyuki
AU - Ozaki, Michitaka
AU - Kamiyama, Toshiya
AU - Todo, Satoru
PY - 2012/6
Y1 - 2012/6
N2 - Since prolonged cold preservation of the heart deteriorates the outcome of heart transplantation, a more protective preservation solution is required. We therefore developed a new solution, named Dsol, and examined whether Dsol, in comparison to UW, could better inhibit myocardial injury resulting from prolonged cold preservation. Syngeneic heterotopic heart transplantation in Lewis rats was performed after cold preservation with UW or Dsol for 24 or 36 h. In addition to graft survival, myocardial injury, ATP content, and Ca 2+ -dependent proteases activity were assessed in the 24-h preservation group. The cytosolic Ca2+ concentration of H9c2 cardiomyocytes after 24-h cold preservation was assessed. Dsol significantly improved 7-day graft survival after 36-h preservation. After 24-h preservation, Dsol was associated with significantly faster recovery of ATP content and less activation of calpain and caspase-3 after reperfusion. Dsol diminished graft injury significantly, as revealed by the lower levels of infarction, apoptosis, serum LDH and AST release, and graft fibrosis at 7-day. Dsol significantly inhibited Ca2+ overload during cold preservation. Dsol inhibited myocardial injury and improved graft survival by suppressing Ca2+ overload during the preservation and the activation of Ca2+ -dependent proteases. Dsol is therefore considered a better alternative to UW to ameliorate the outcome of heart transplantation.
AB - Since prolonged cold preservation of the heart deteriorates the outcome of heart transplantation, a more protective preservation solution is required. We therefore developed a new solution, named Dsol, and examined whether Dsol, in comparison to UW, could better inhibit myocardial injury resulting from prolonged cold preservation. Syngeneic heterotopic heart transplantation in Lewis rats was performed after cold preservation with UW or Dsol for 24 or 36 h. In addition to graft survival, myocardial injury, ATP content, and Ca 2+ -dependent proteases activity were assessed in the 24-h preservation group. The cytosolic Ca2+ concentration of H9c2 cardiomyocytes after 24-h cold preservation was assessed. Dsol significantly improved 7-day graft survival after 36-h preservation. After 24-h preservation, Dsol was associated with significantly faster recovery of ATP content and less activation of calpain and caspase-3 after reperfusion. Dsol diminished graft injury significantly, as revealed by the lower levels of infarction, apoptosis, serum LDH and AST release, and graft fibrosis at 7-day. Dsol significantly inhibited Ca2+ overload during cold preservation. Dsol inhibited myocardial injury and improved graft survival by suppressing Ca2+ overload during the preservation and the activation of Ca2+ -dependent proteases. Dsol is therefore considered a better alternative to UW to ameliorate the outcome of heart transplantation.
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U2 - 10.1111/j.1432-2277.2012.01469.x
DO - 10.1111/j.1432-2277.2012.01469.x
M3 - Article
C2 - 22471391
AN - SCOPUS:84861183876
SN - 0934-0874
VL - 25
SP - 696
EP - 706
JO - Transplant International
JF - Transplant International
IS - 6
ER -