Superoxide anion mediates pulmonary vascular permeability caused by neutrophils in cardiopulmonary bypass

Tatsuo Tanita, Chun Song, Hiroshi Kubo, Yasushi Hoshikawa, Masayuki Chida, Satoshi Suzuki, Sadafumi Ono, Shigefumi Fujimura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

During cardiopulmonary bypass (CPB), neutrophils (PMNs) may be stimulated by shear stress which could contribute to the pulmonary injury that occurs after CPB. To elucidate whether mechanically stimulated PMNs increase pulmonary vascular permeability, measured as the pulmonary filtration coefficient (K) and pulmonary vascular resistance, and to elucidate whether superoxide anion mediates this increase, we assessed the effects of stimulated and unstimulated PMNs, and of superoxide dismutase (SOD) on K and resistance in isolated perfused lungs from Sprague-Dawley rats. PMNs were stimulated by gentle agitation in a glass vial for 10s. Lungs perfused with the stimulated PMNs, being the stimulated group (n = 6), elicited a 5-fold increase in the filtration coefficient compared with lungs perfused with unstimulated cells, being the unstimulated group (n = 6). This increase in filtration was completely blocked by the preincubation of stimulated PMNs with CD18 monoclonal anti-body, being the Ab group (n = 6), and also by superoxide dismutase, being the SOD group (n = 6). Pulmonary vascular resistance was not increased by stimulated PMNs, and the accumulation of stimulated PMNs was not blocked by SOD. These findings suggest that stimulated PMNs increase K and that superoxide anion may injure the pulmonary vascular endothelial cells.

Original languageEnglish
Pages (from-to)755-761
Number of pages7
JournalSurgery Today
Volume29
Issue number8
DOIs
Publication statusPublished - 03-09-1999

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Capillary Permeability
Cardiopulmonary Bypass
Superoxides
Neutrophils
Lung
Superoxide Dismutase
Vascular Resistance
Lung Injury
Glass
Sprague Dawley Rats
Endothelial Cells

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Tanita, Tatsuo ; Song, Chun ; Kubo, Hiroshi ; Hoshikawa, Yasushi ; Chida, Masayuki ; Suzuki, Satoshi ; Ono, Sadafumi ; Fujimura, Shigefumi. / Superoxide anion mediates pulmonary vascular permeability caused by neutrophils in cardiopulmonary bypass. In: Surgery Today. 1999 ; Vol. 29, No. 8. pp. 755-761.
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Superoxide anion mediates pulmonary vascular permeability caused by neutrophils in cardiopulmonary bypass. / Tanita, Tatsuo; Song, Chun; Kubo, Hiroshi; Hoshikawa, Yasushi; Chida, Masayuki; Suzuki, Satoshi; Ono, Sadafumi; Fujimura, Shigefumi.

In: Surgery Today, Vol. 29, No. 8, 03.09.1999, p. 755-761.

Research output: Contribution to journalArticle

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