TY - JOUR
T1 - Superoxide production from human polymorphonuclear leukocytes by human mannan-binding protein (MBP)
AU - Uemura, Kazuhide
AU - Yamamoto, Harumi
AU - Nakagawa, Tomoaki
AU - Nakamura, Kishiko
AU - Kawasaki, Nobuko
AU - Oka, Shogo
AU - Ma, Bruce Yong
AU - Kawasaki, Toshisuke
PY - 2004
Y1 - 2004
N2 - Mannan-binding protein (MBP) is a Ca 2+-dependent mammalian lectin that plays an important role in innate immunity. In this study, we found that ligand-bound MBP stimulates polymorphonuclear leukocytes (PMN) to induce cell aggregation and superoxide production. The biological response of PMN to ligand-bound MBP was dose- and time-dependent. The PMN aggregation and superoxide production induced by ligand-bound MBP was blocked completely by pertussis toxin, and partially blocked by a platelet activation factor receptor antagonist, TCV-309. These findings suggest that the ligand-bound MBP stimulates PMN through a putative MBP receptor(s) on PMN.
AB - Mannan-binding protein (MBP) is a Ca 2+-dependent mammalian lectin that plays an important role in innate immunity. In this study, we found that ligand-bound MBP stimulates polymorphonuclear leukocytes (PMN) to induce cell aggregation and superoxide production. The biological response of PMN to ligand-bound MBP was dose- and time-dependent. The PMN aggregation and superoxide production induced by ligand-bound MBP was blocked completely by pertussis toxin, and partially blocked by a platelet activation factor receptor antagonist, TCV-309. These findings suggest that the ligand-bound MBP stimulates PMN through a putative MBP receptor(s) on PMN.
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U2 - 10.1023/B:GLYC.0000043753.11090.88
DO - 10.1023/B:GLYC.0000043753.11090.88
M3 - Article
C2 - 15467404
AN - SCOPUS:5444225218
SN - 0282-0080
VL - 21
SP - 79
EP - 84
JO - Glycoconjugate Journal
JF - Glycoconjugate Journal
IS - 1-2
ER -