Suppressed expression of GTP cyclohydrolase I mRNA and accelerated expression of inducible nitric oxide synthase mRNA in endomyocardial biopsy specimens from patients with dilated cardiomyopathy

Masatsugu Ohtsuki, Takahide Nomura, Shin Ichiro Morimoto, Shinya Hiramitsu, Akihisa Uemura, Shigeru Kato, Yasuchika Kato, Hiroaki Shiraishi, Junko Shimokubo, Naoki Yamamoto, Hitoshi Hishida

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Tetrahydrobiopterin (BH4) is an essential cofactor of nitric oxide synthase, and GTP cyclohydrolase I (GCHI) is a rate-limiting enzyme in the biosynthesis of BH4. The expression of inducible nitric oxide synthase (iNOS) was earlier demonstrated in the ventricles of patients with dilated cardiomyopathy (DCM) although that of GCHI was not clarified. The present study was designed to determine the GCHI mRNA expression as well as to confirm iNOS mRNA expression in endomyocardial biopsy specimens from patients with DCM. Methods: Clinical details were assessed in 19 patients with DCM and in 9 control subjects. The real-time reverse transcription polymerase chain reaction (PCR) was performed on total RNA extracted from endomyocardial biopsy specimens. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA was quantified for use as an internal control. Results: iNOS/GAPDH for the DCM samples was 4.8-fold greater than that for the control ones (P<0.01), whereas the GCHI/GAPDH for the DCM samples was reduced to 31.1% of the control (P<0.05). Conclusions: The increased expression of iNOS mRNA was confirmed in endomyocardial biopsy specimens from patients with DCM. The GCHI mRNA level was suppressed in these specimens.

Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalClinica Chimica Acta
Volume353
Issue number1-2
DOIs
Publication statusPublished - 01-01-2005

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GTP Cyclohydrolase
Biopsy
Dilated Cardiomyopathy
Nitric Oxide Synthase Type II
Glyceraldehyde-3-Phosphate Dehydrogenases
Messenger RNA
Polymerase chain reaction
Biosynthesis
Transcription
Nitric Oxide Synthase
Reverse Transcription
RNA
Enzymes
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Ohtsuki, Masatsugu ; Nomura, Takahide ; Morimoto, Shin Ichiro ; Hiramitsu, Shinya ; Uemura, Akihisa ; Kato, Shigeru ; Kato, Yasuchika ; Shiraishi, Hiroaki ; Shimokubo, Junko ; Yamamoto, Naoki ; Hishida, Hitoshi. / Suppressed expression of GTP cyclohydrolase I mRNA and accelerated expression of inducible nitric oxide synthase mRNA in endomyocardial biopsy specimens from patients with dilated cardiomyopathy. In: Clinica Chimica Acta. 2005 ; Vol. 353, No. 1-2. pp. 103-107.
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title = "Suppressed expression of GTP cyclohydrolase I mRNA and accelerated expression of inducible nitric oxide synthase mRNA in endomyocardial biopsy specimens from patients with dilated cardiomyopathy",
abstract = "Background: Tetrahydrobiopterin (BH4) is an essential cofactor of nitric oxide synthase, and GTP cyclohydrolase I (GCHI) is a rate-limiting enzyme in the biosynthesis of BH4. The expression of inducible nitric oxide synthase (iNOS) was earlier demonstrated in the ventricles of patients with dilated cardiomyopathy (DCM) although that of GCHI was not clarified. The present study was designed to determine the GCHI mRNA expression as well as to confirm iNOS mRNA expression in endomyocardial biopsy specimens from patients with DCM. Methods: Clinical details were assessed in 19 patients with DCM and in 9 control subjects. The real-time reverse transcription polymerase chain reaction (PCR) was performed on total RNA extracted from endomyocardial biopsy specimens. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA was quantified for use as an internal control. Results: iNOS/GAPDH for the DCM samples was 4.8-fold greater than that for the control ones (P<0.01), whereas the GCHI/GAPDH for the DCM samples was reduced to 31.1{\%} of the control (P<0.05). Conclusions: The increased expression of iNOS mRNA was confirmed in endomyocardial biopsy specimens from patients with DCM. The GCHI mRNA level was suppressed in these specimens.",
author = "Masatsugu Ohtsuki and Takahide Nomura and Morimoto, {Shin Ichiro} and Shinya Hiramitsu and Akihisa Uemura and Shigeru Kato and Yasuchika Kato and Hiroaki Shiraishi and Junko Shimokubo and Naoki Yamamoto and Hitoshi Hishida",
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Suppressed expression of GTP cyclohydrolase I mRNA and accelerated expression of inducible nitric oxide synthase mRNA in endomyocardial biopsy specimens from patients with dilated cardiomyopathy. / Ohtsuki, Masatsugu; Nomura, Takahide; Morimoto, Shin Ichiro; Hiramitsu, Shinya; Uemura, Akihisa; Kato, Shigeru; Kato, Yasuchika; Shiraishi, Hiroaki; Shimokubo, Junko; Yamamoto, Naoki; Hishida, Hitoshi.

In: Clinica Chimica Acta, Vol. 353, No. 1-2, 01.01.2005, p. 103-107.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Suppressed expression of GTP cyclohydrolase I mRNA and accelerated expression of inducible nitric oxide synthase mRNA in endomyocardial biopsy specimens from patients with dilated cardiomyopathy

AU - Ohtsuki, Masatsugu

AU - Nomura, Takahide

AU - Morimoto, Shin Ichiro

AU - Hiramitsu, Shinya

AU - Uemura, Akihisa

AU - Kato, Shigeru

AU - Kato, Yasuchika

AU - Shiraishi, Hiroaki

AU - Shimokubo, Junko

AU - Yamamoto, Naoki

AU - Hishida, Hitoshi

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Background: Tetrahydrobiopterin (BH4) is an essential cofactor of nitric oxide synthase, and GTP cyclohydrolase I (GCHI) is a rate-limiting enzyme in the biosynthesis of BH4. The expression of inducible nitric oxide synthase (iNOS) was earlier demonstrated in the ventricles of patients with dilated cardiomyopathy (DCM) although that of GCHI was not clarified. The present study was designed to determine the GCHI mRNA expression as well as to confirm iNOS mRNA expression in endomyocardial biopsy specimens from patients with DCM. Methods: Clinical details were assessed in 19 patients with DCM and in 9 control subjects. The real-time reverse transcription polymerase chain reaction (PCR) was performed on total RNA extracted from endomyocardial biopsy specimens. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA was quantified for use as an internal control. Results: iNOS/GAPDH for the DCM samples was 4.8-fold greater than that for the control ones (P<0.01), whereas the GCHI/GAPDH for the DCM samples was reduced to 31.1% of the control (P<0.05). Conclusions: The increased expression of iNOS mRNA was confirmed in endomyocardial biopsy specimens from patients with DCM. The GCHI mRNA level was suppressed in these specimens.

AB - Background: Tetrahydrobiopterin (BH4) is an essential cofactor of nitric oxide synthase, and GTP cyclohydrolase I (GCHI) is a rate-limiting enzyme in the biosynthesis of BH4. The expression of inducible nitric oxide synthase (iNOS) was earlier demonstrated in the ventricles of patients with dilated cardiomyopathy (DCM) although that of GCHI was not clarified. The present study was designed to determine the GCHI mRNA expression as well as to confirm iNOS mRNA expression in endomyocardial biopsy specimens from patients with DCM. Methods: Clinical details were assessed in 19 patients with DCM and in 9 control subjects. The real-time reverse transcription polymerase chain reaction (PCR) was performed on total RNA extracted from endomyocardial biopsy specimens. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA was quantified for use as an internal control. Results: iNOS/GAPDH for the DCM samples was 4.8-fold greater than that for the control ones (P<0.01), whereas the GCHI/GAPDH for the DCM samples was reduced to 31.1% of the control (P<0.05). Conclusions: The increased expression of iNOS mRNA was confirmed in endomyocardial biopsy specimens from patients with DCM. The GCHI mRNA level was suppressed in these specimens.

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