Suppression of GABA-induced chloride current by 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2)

Yoshikatsu Kanai; Toshihiko Aosaki; Osamu Wada; Shigeo Manabe

doi:10.1016/0014-2999(89)90375-0

Suppression of GABA-induced chloride current by 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2)

Yoshikatsu Kanai, Toshihiko Aosaki, Osamu Wada, Shigeo Manabe

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The effect of a convulsive agent, 3-amino-1-methyl-5H-pyridol[4,3-b]indole (Trp-P-2), on the GABA-induced chloride current (I_C1) in dissociated mouse sensory neurons was investigated using the whole cell clamp method. Trp-P-2 reversibly suppressed the GABA-induced I_Cl in a dose-dependent manner. The IC₅₀ for Trp-P-2 on the I_Cl evoked by 3 μM GABA was 11.1 μM. Ro15-1788 had no effect on the suppressive action of Trp-P-2. These results suggest that the observed effect of Trp-P-2 is mediated by its action as antagonist at GABA_A receptors.

Original language	English
Pages (from-to)	553-556
Number of pages	4
Journal	European Journal of Pharmacology
Volume	166
Issue number	3
DOIs	https://doi.org/10.1016/0014-2999(89)90375-0
Publication status	Published - 03-08-1989
Externally published	Yes

All Science Journal Classification (ASJC) codes

Pharmacology

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10.1016/0014-2999(89)90375-0

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title = "Suppression of GABA-induced chloride current by 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2)",

abstract = "The effect of a convulsive agent, 3-amino-1-methyl-5H-pyridol[4,3-b]indole (Trp-P-2), on the GABA-induced chloride current (IC1) in dissociated mouse sensory neurons was investigated using the whole cell clamp method. Trp-P-2 reversibly suppressed the GABA-induced ICl in a dose-dependent manner. The IC50 for Trp-P-2 on the ICl evoked by 3 μM GABA was 11.1 μM. Ro15-1788 had no effect on the suppressive action of Trp-P-2. These results suggest that the observed effect of Trp-P-2 is mediated by its action as antagonist at GABAA receptors.",

keywords = "Amino-γ-carboline, Sensory neuron (Whole cell clamp, Mouse), γ-Aminobutyric acid",

author = "Yoshikatsu Kanai and Toshihiko Aosaki and Osamu Wada and Shigeo Manabe",

year = "1989",

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doi = "10.1016/0014-2999(89)90375-0",

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T1 - Suppression of GABA-induced chloride current by 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2)

AU - Kanai, Yoshikatsu

AU - Aosaki, Toshihiko

AU - Wada, Osamu

AU - Manabe, Shigeo

PY - 1989/8/3

Y1 - 1989/8/3

N2 - The effect of a convulsive agent, 3-amino-1-methyl-5H-pyridol[4,3-b]indole (Trp-P-2), on the GABA-induced chloride current (IC1) in dissociated mouse sensory neurons was investigated using the whole cell clamp method. Trp-P-2 reversibly suppressed the GABA-induced ICl in a dose-dependent manner. The IC50 for Trp-P-2 on the ICl evoked by 3 μM GABA was 11.1 μM. Ro15-1788 had no effect on the suppressive action of Trp-P-2. These results suggest that the observed effect of Trp-P-2 is mediated by its action as antagonist at GABAA receptors.

AB - The effect of a convulsive agent, 3-amino-1-methyl-5H-pyridol[4,3-b]indole (Trp-P-2), on the GABA-induced chloride current (IC1) in dissociated mouse sensory neurons was investigated using the whole cell clamp method. Trp-P-2 reversibly suppressed the GABA-induced ICl in a dose-dependent manner. The IC50 for Trp-P-2 on the ICl evoked by 3 μM GABA was 11.1 μM. Ro15-1788 had no effect on the suppressive action of Trp-P-2. These results suggest that the observed effect of Trp-P-2 is mediated by its action as antagonist at GABAA receptors.

KW - Amino-γ-carboline

KW - Sensory neuron (Whole cell clamp, Mouse)

KW - γ-Aminobutyric acid

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SN - 0014-2999

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JO - European Journal of Pharmacology

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IS - 3

ER -

Suppression of GABA-induced chloride current by 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2)

Abstract

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