Suppression of human immunodeficiency virus type 1 replication by arginine deiminase of Mycoplasma arginini

Makoto Kubo, Hironori Nishitsuji, Kiyoshi Kurihara, Takaya Hayashi, Takao Masuda, Mari Kannagi

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17 Citations (Scopus)


It was found previously that human immunodeficiency virus type 1 (HIV-1)-irrelevant CD8+ cytotoxic T lymphocytes (CTLs) from uninfected donors suppressed HIV-1 replication in a cell-contact-dependent manner. However, one of these CTL lines (CTL-3) also significantly suppressed HIV-1 replication through its supernatant. Here, the suppressive fraction from CTL-3 supernatant was purified and analysed by mass spectrometry. A protein band specific for the suppressive fraction was identified as arginine deiminase from Mycoplasma arginini, which catalyses the hydrolysis of arginine to citrulline. Addition of L-arginine or the use of antibiotics against mycoplasma restored supernatant-mediated but not cell-contact-dependent suppression of HIV-1 replication by CTL-3, clearly indicating that arginine deiminase of M. arginini in the supernatants suppressed HIV-1 replication, which is independent of CD8+ T-cell-mediated HIV-1 suppression via cell contact. Arginine deiminase is known to be a chemotherapeutic agent against arginine-requiring tumours and these results suggest that it also has potential application in antiviral therapy.

Original languageEnglish
Pages (from-to)1589-1593
Number of pages5
JournalJournal of General Virology
Issue number6
Publication statusPublished - 06-2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Virology


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