Suppression of inflammation during cell-free concentrated ascites reinfusion therapy using a blood purification device

Research output: Contribution to journalArticlepeer-review

Abstract

In recent years, cell-free concentrated ascites reinfusion therapy has been used to treat patients with malignant ascites. However, concentrated ascites reinfusion therapy involves enrichment and reinfusion of useful proteins and inflammatory cytokines. Therefore, fever is a primary side effect and significant problem for patients with ascites. We removed IL-6, an inflammatory cytokine, by mixing malignant ascites and the hexadecyl group adsorbent from a β2-microglobulin-adsorbing column (Lixelle S-15). As a result, the hexadecyl group adsorbent did not adsorb the albumin of malignant ascites but adsorbed 43% of IL-6. To investigate the effect of the hexadecyl group adsorbent on hepatocytes, the adsorbed ascites was added to a human hepatoma cell line (HepG2), and the gene expression levels of albumin and serum amyloid A protein were examined. After absorption, ascites showed significantly suppressed serum amyloid A protein expression and significantly increased albumin gene expression compared to before adsorption. Our results suggest that incorporation of Lixelle to filter and concentrate malignant ascites can suppress inflammatory responses and reduce the inhibition of albumin synthesis in the liver after reinfusion.

Original languageEnglish
Pages (from-to)511-515
Number of pages5
JournalTherapeutic Apheresis and Dialysis
Volume24
Issue number5
DOIs
Publication statusPublished - 01-10-2020

All Science Journal Classification (ASJC) codes

  • Hematology
  • Nephrology

Fingerprint Dive into the research topics of 'Suppression of inflammation during cell-free concentrated ascites reinfusion therapy using a blood purification device'. Together they form a unique fingerprint.

Cite this