TY - JOUR
T1 - Suppression of invasion and peritoneal carcinomatosis of ovarian cancer cells by overexpression of AP-2α
AU - Sumigama, Seiji
AU - Ito, Tomomi
AU - Kajiyama, Hiroaki
AU - Shibata, Kiyosumi
AU - Tamakoshi, Koji
AU - Kikkawa, Fumitaka
AU - Williams, Trevor
AU - Tainsky, Michael A.
AU - Nomura, Seiji
AU - Mizutani, Shigehiko
N1 - Funding Information:
This study was partly supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan, from R01CA053475, and from the Ministry of Public Management, Home Affairs, Posts and Telec ommuni-cations of Japan for specific medical research (in collaboration with Nagoya Teishin Hospital).
PY - 2004/7/15
Y1 - 2004/7/15
N2 - A previous report demonstrated that AP-2α favors the survival of ovarian cancer patients by clinical findings. However, the functional roles of AP-2α in human ovarian cancers have not been determined. To clarify the roles, we overexpressed AP-2α in SKOV3 human ovarian cancer cells, which originally possess little AP-2α. AP-2α overexpression changed cell morphology from spindle to epithelioid type and suppressed cell proliferation and invasion, which would be partially correlated with decreased phosphorylation levels of the erbB2, Akt and ERK pathways, increased E-cadherin and reduced pro-matrix metalloproteinase-2 levels. Moreover, nude mice intraperitoneally injected with AP-2α-overexpressing cells survived longer than those with neotransfected cells. The present data represent the first direct evidence that AP-2α plays a tumor suppressive role in ovarian cancer.
AB - A previous report demonstrated that AP-2α favors the survival of ovarian cancer patients by clinical findings. However, the functional roles of AP-2α in human ovarian cancers have not been determined. To clarify the roles, we overexpressed AP-2α in SKOV3 human ovarian cancer cells, which originally possess little AP-2α. AP-2α overexpression changed cell morphology from spindle to epithelioid type and suppressed cell proliferation and invasion, which would be partially correlated with decreased phosphorylation levels of the erbB2, Akt and ERK pathways, increased E-cadherin and reduced pro-matrix metalloproteinase-2 levels. Moreover, nude mice intraperitoneally injected with AP-2α-overexpressing cells survived longer than those with neotransfected cells. The present data represent the first direct evidence that AP-2α plays a tumor suppressive role in ovarian cancer.
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U2 - 10.1038/sj.onc.1207723
DO - 10.1038/sj.onc.1207723
M3 - Article
C2 - 15146170
AN - SCOPUS:3843146276
SN - 0950-9232
VL - 23
SP - 5496
EP - 5504
JO - Oncogene
JF - Oncogene
IS - 32
ER -
