Suppression of matrix metalloproteinase-3 synthesis by interleukin-4 in human articular chondrocytes

Osamu Nemoto, Harumoto Yamada, Toshiyuki Kikuchi, Masayuki Shimmei, Ken'ichi Obata, Hiroshi Sato, Motoharu Seiki

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)


Objective. To evaluate the effect of interleukin-4 (IL-4) on IL-1 induced matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) production by human articular chondrocytes. Methods. Monolayer cell culture of chondrocytes was obtained from human articular cartilage from patella within 24 h after death. MMP-3 and TIMP-1 protein levels were determined by ELISA. MMP-3 activity was assayed as caseinase activity. Amounts of MMP-3 and TIMP-1 mRNA were measured by Northern blot analysis. Results. IL-4 suppressed IL-1 stimulated MMP-3 protein and enzyme activity. Moreover, IL-4 suppressed IL-1 induced MMP-3 mRNA. In contrast, IL-4 did not alter the level of TIMP-1 protein and mRNA. Conclusion. IL-4 may be implicated as a protective mediator of joint destruction seen in inflammatory arthritis.

Original languageEnglish
Pages (from-to)1774-1779
Number of pages6
JournalJournal of Rheumatology
Issue number9
Publication statusPublished - 09-1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Immunology and Allergy
  • Immunology


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