Suppression of matrix metalloproteinase-3 synthesis by interleukin-4 in human articular chondrocytes

Osamu Nemoto, Harumoto Yamada, Toshiyuki Kikuchi, Masayuki Shimmei, Ken'ichi Obata, Hiroshi Sato, Motoharu Seiki

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Objective. To evaluate the effect of interleukin-4 (IL-4) on IL-1 induced matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) production by human articular chondrocytes. Methods. Monolayer cell culture of chondrocytes was obtained from human articular cartilage from patella within 24 h after death. MMP-3 and TIMP-1 protein levels were determined by ELISA. MMP-3 activity was assayed as caseinase activity. Amounts of MMP-3 and TIMP-1 mRNA were measured by Northern blot analysis. Results. IL-4 suppressed IL-1 stimulated MMP-3 protein and enzyme activity. Moreover, IL-4 suppressed IL-1 induced MMP-3 mRNA. In contrast, IL-4 did not alter the level of TIMP-1 protein and mRNA. Conclusion. IL-4 may be implicated as a protective mediator of joint destruction seen in inflammatory arthritis.

Original languageEnglish
Pages (from-to)1774-1779
Number of pages6
JournalJournal of Rheumatology
Volume24
Issue number9
Publication statusPublished - 01-09-1997

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Matrix Metalloproteinase 3
Chondrocytes
Interleukin-4
Tissue Inhibitor of Metalloproteinase-1
Joints
Matrix Metalloproteinase Inhibitors
Interleukin-1
Messenger RNA
Proteins
Patella
Articular Cartilage
Northern Blotting
Arthritis
Cell Culture Techniques
Enzyme-Linked Immunosorbent Assay
Enzymes

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Immunology and Allergy
  • Immunology

Cite this

Nemoto, O., Yamada, H., Kikuchi, T., Shimmei, M., Obata, K., Sato, H., & Seiki, M. (1997). Suppression of matrix metalloproteinase-3 synthesis by interleukin-4 in human articular chondrocytes. Journal of Rheumatology, 24(9), 1774-1779.
Nemoto, Osamu ; Yamada, Harumoto ; Kikuchi, Toshiyuki ; Shimmei, Masayuki ; Obata, Ken'ichi ; Sato, Hiroshi ; Seiki, Motoharu. / Suppression of matrix metalloproteinase-3 synthesis by interleukin-4 in human articular chondrocytes. In: Journal of Rheumatology. 1997 ; Vol. 24, No. 9. pp. 1774-1779.
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abstract = "Objective. To evaluate the effect of interleukin-4 (IL-4) on IL-1 induced matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) production by human articular chondrocytes. Methods. Monolayer cell culture of chondrocytes was obtained from human articular cartilage from patella within 24 h after death. MMP-3 and TIMP-1 protein levels were determined by ELISA. MMP-3 activity was assayed as caseinase activity. Amounts of MMP-3 and TIMP-1 mRNA were measured by Northern blot analysis. Results. IL-4 suppressed IL-1 stimulated MMP-3 protein and enzyme activity. Moreover, IL-4 suppressed IL-1 induced MMP-3 mRNA. In contrast, IL-4 did not alter the level of TIMP-1 protein and mRNA. Conclusion. IL-4 may be implicated as a protective mediator of joint destruction seen in inflammatory arthritis.",
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Nemoto, O, Yamada, H, Kikuchi, T, Shimmei, M, Obata, K, Sato, H & Seiki, M 1997, 'Suppression of matrix metalloproteinase-3 synthesis by interleukin-4 in human articular chondrocytes', Journal of Rheumatology, vol. 24, no. 9, pp. 1774-1779.

Suppression of matrix metalloproteinase-3 synthesis by interleukin-4 in human articular chondrocytes. / Nemoto, Osamu; Yamada, Harumoto; Kikuchi, Toshiyuki; Shimmei, Masayuki; Obata, Ken'ichi; Sato, Hiroshi; Seiki, Motoharu.

In: Journal of Rheumatology, Vol. 24, No. 9, 01.09.1997, p. 1774-1779.

Research output: Contribution to journalArticle

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T1 - Suppression of matrix metalloproteinase-3 synthesis by interleukin-4 in human articular chondrocytes

AU - Nemoto, Osamu

AU - Yamada, Harumoto

AU - Kikuchi, Toshiyuki

AU - Shimmei, Masayuki

AU - Obata, Ken'ichi

AU - Sato, Hiroshi

AU - Seiki, Motoharu

PY - 1997/9/1

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N2 - Objective. To evaluate the effect of interleukin-4 (IL-4) on IL-1 induced matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) production by human articular chondrocytes. Methods. Monolayer cell culture of chondrocytes was obtained from human articular cartilage from patella within 24 h after death. MMP-3 and TIMP-1 protein levels were determined by ELISA. MMP-3 activity was assayed as caseinase activity. Amounts of MMP-3 and TIMP-1 mRNA were measured by Northern blot analysis. Results. IL-4 suppressed IL-1 stimulated MMP-3 protein and enzyme activity. Moreover, IL-4 suppressed IL-1 induced MMP-3 mRNA. In contrast, IL-4 did not alter the level of TIMP-1 protein and mRNA. Conclusion. IL-4 may be implicated as a protective mediator of joint destruction seen in inflammatory arthritis.

AB - Objective. To evaluate the effect of interleukin-4 (IL-4) on IL-1 induced matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) production by human articular chondrocytes. Methods. Monolayer cell culture of chondrocytes was obtained from human articular cartilage from patella within 24 h after death. MMP-3 and TIMP-1 protein levels were determined by ELISA. MMP-3 activity was assayed as caseinase activity. Amounts of MMP-3 and TIMP-1 mRNA were measured by Northern blot analysis. Results. IL-4 suppressed IL-1 stimulated MMP-3 protein and enzyme activity. Moreover, IL-4 suppressed IL-1 induced MMP-3 mRNA. In contrast, IL-4 did not alter the level of TIMP-1 protein and mRNA. Conclusion. IL-4 may be implicated as a protective mediator of joint destruction seen in inflammatory arthritis.

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