Surgical invasiveness of cell transplantation into the guinea pig cochlear modiolus

Hideaki Ogita; Takayuki Nakagawa; Kyu Yup Lee; Takatoshi Inaoka; Takayuki Okano; Yayoi S. Kikkawa; Tatsunori Sakamoto; Juichi Ito

doi:10.1159/000165915

Surgical invasiveness of cell transplantation into the guinea pig cochlear modiolus

Hideaki Ogita, Takayuki Nakagawa, Kyu Yup Lee, Takatoshi Inaoka, Takayuki Okano, Yayoi S. Kikkawa, Tatsunori Sakamoto, Juichi Ito

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Objective: Previous studies have demonstrated the potential of cell transplantation for regeneration of spiral ganglion neurons (SGNs). However, the effect of surgical invasion on host cochleae has yet to be evaluated. The present study investigated the efficiency and invasiveness of our surgical procedure using a fine glass pipette for injections into the cochlear modiolus. Methods: We examined the survival of transplanted embryonic stem cell-derived neurons in the cochlear modiolus of guinea pigs. Surgical invasiveness was assessed by measurements of electrically evoked auditory brainstem responses (eABRs) and SGN densities after an injection of 5 ml of saline into the cochlear modiolus. Results: All of the transplanted animals exhibited localization of transplanted cells in the cochlear modiolus. No significant alterations in the eABR thresholds or SGN densities were found following surgery. Conclusion: These findings indicate that our procedure is a viable method for testing the potential of transplants for SGN replacement.

Original language	English
Pages (from-to)	32-39
Number of pages	8
Journal	ORL
Volume	71
Issue number	1
DOIs	https://doi.org/10.1159/000165915
Publication status	Published - 12-2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Otorhinolaryngology

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10.1159/000165915

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title = "Surgical invasiveness of cell transplantation into the guinea pig cochlear modiolus",

abstract = "Objective: Previous studies have demonstrated the potential of cell transplantation for regeneration of spiral ganglion neurons (SGNs). However, the effect of surgical invasion on host cochleae has yet to be evaluated. The present study investigated the efficiency and invasiveness of our surgical procedure using a fine glass pipette for injections into the cochlear modiolus. Methods: We examined the survival of transplanted embryonic stem cell-derived neurons in the cochlear modiolus of guinea pigs. Surgical invasiveness was assessed by measurements of electrically evoked auditory brainstem responses (eABRs) and SGN densities after an injection of 5 ml of saline into the cochlear modiolus. Results: All of the transplanted animals exhibited localization of transplanted cells in the cochlear modiolus. No significant alterations in the eABR thresholds or SGN densities were found following surgery. Conclusion: These findings indicate that our procedure is a viable method for testing the potential of transplants for SGN replacement.",

author = "Hideaki Ogita and Takayuki Nakagawa and Lee, {Kyu Yup} and Takatoshi Inaoka and Takayuki Okano and Kikkawa, {Yayoi S.} and Tatsunori Sakamoto and Juichi Ito",

year = "2008",

month = dec,

doi = "10.1159/000165915",

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AU - Ogita, Hideaki

AU - Nakagawa, Takayuki

AU - Lee, Kyu Yup

AU - Inaoka, Takatoshi

AU - Okano, Takayuki

AU - Kikkawa, Yayoi S.

AU - Sakamoto, Tatsunori

AU - Ito, Juichi

PY - 2008/12

Y1 - 2008/12

N2 - Objective: Previous studies have demonstrated the potential of cell transplantation for regeneration of spiral ganglion neurons (SGNs). However, the effect of surgical invasion on host cochleae has yet to be evaluated. The present study investigated the efficiency and invasiveness of our surgical procedure using a fine glass pipette for injections into the cochlear modiolus. Methods: We examined the survival of transplanted embryonic stem cell-derived neurons in the cochlear modiolus of guinea pigs. Surgical invasiveness was assessed by measurements of electrically evoked auditory brainstem responses (eABRs) and SGN densities after an injection of 5 ml of saline into the cochlear modiolus. Results: All of the transplanted animals exhibited localization of transplanted cells in the cochlear modiolus. No significant alterations in the eABR thresholds or SGN densities were found following surgery. Conclusion: These findings indicate that our procedure is a viable method for testing the potential of transplants for SGN replacement.

AB - Objective: Previous studies have demonstrated the potential of cell transplantation for regeneration of spiral ganglion neurons (SGNs). However, the effect of surgical invasion on host cochleae has yet to be evaluated. The present study investigated the efficiency and invasiveness of our surgical procedure using a fine glass pipette for injections into the cochlear modiolus. Methods: We examined the survival of transplanted embryonic stem cell-derived neurons in the cochlear modiolus of guinea pigs. Surgical invasiveness was assessed by measurements of electrically evoked auditory brainstem responses (eABRs) and SGN densities after an injection of 5 ml of saline into the cochlear modiolus. Results: All of the transplanted animals exhibited localization of transplanted cells in the cochlear modiolus. No significant alterations in the eABR thresholds or SGN densities were found following surgery. Conclusion: These findings indicate that our procedure is a viable method for testing the potential of transplants for SGN replacement.

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Surgical invasiveness of cell transplantation into the guinea pig cochlear modiolus

Abstract

All Science Journal Classification (ASJC) codes

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