TY - JOUR
T1 - Survey of human immunodeficiency virus (HIV)-seropositive patients with mycobacterial infection in Japan
AU - Otsuka, Yayoi
AU - Fujino, Tomoko
AU - Mori, Namiko
AU - Sekiguchi, Jun Ichiro
AU - Toyota, Emiko
AU - Saruta, Katsutoshi
AU - Kikuchi, Yoshihiro
AU - Sasaki, Yuka
AU - Ajisawa, Atsushi
AU - Otsuka, Yoshito
AU - Nagai, Hideaki
AU - Takahara, Makoto
AU - Saka, Hideo
AU - Shirasaka, Takuma
AU - Yamashita, Yasuki
AU - Kiyosuke, Makiko
AU - Koga, Hideyuki
AU - Oka, Shinichi
AU - Kimura, Satoshi
AU - Mori, Toru
AU - Kuratsuji, Tadatoshi
AU - Kirikae, Teruo
PY - 2005/12
Y1 - 2005/12
N2 - Objective: To assess DNA polymorphisms in mycobacterial isolates obtained from human immunodeficiency virus (HIV)-seropositive patients with tuberculosis in Japan from 1996 to 2003. Methods: Restriction fragment length polymorphisms (RFLP) from Mycobacterium tuberculosis and Mycobacterium avium isolates obtained from individual seropositive patients with tuberculosis (n=78) were analysed with the use of IS6110 and (CGG)5 or IS1245 and IS1311, respectively, as markers. As a control, the same procedures were applied to isolates from HIV-seronegative tuberculosis patients (n=87). Results: Of 86 mycobacterial strains, M. tuberculosis, M. avium and Mycobacterium chelonae were identified in 48 (55.8%), 36 (41.9%) and 2 (2.3%) isolates, respectively. The obtained RFLP patterns of M. tuberculosis isolates from both the HIV-seropositive and -seronegative groups were variable, suggesting no obvious clustering among the isolates. Similar results were obtained in isolates of M. avium. Conclusions: This is the first report on the molecular epidemiology of Mycobacterium spp. isolated from HIV-seropositive patients in Japan. The results indicate that no particular clones of M. tuberculosis or M. avium prevail in HIV-seropositive patients in Japan. Further monitoring of mycobacterial infection associated with HIV infection in Japan should be continued.
AB - Objective: To assess DNA polymorphisms in mycobacterial isolates obtained from human immunodeficiency virus (HIV)-seropositive patients with tuberculosis in Japan from 1996 to 2003. Methods: Restriction fragment length polymorphisms (RFLP) from Mycobacterium tuberculosis and Mycobacterium avium isolates obtained from individual seropositive patients with tuberculosis (n=78) were analysed with the use of IS6110 and (CGG)5 or IS1245 and IS1311, respectively, as markers. As a control, the same procedures were applied to isolates from HIV-seronegative tuberculosis patients (n=87). Results: Of 86 mycobacterial strains, M. tuberculosis, M. avium and Mycobacterium chelonae were identified in 48 (55.8%), 36 (41.9%) and 2 (2.3%) isolates, respectively. The obtained RFLP patterns of M. tuberculosis isolates from both the HIV-seropositive and -seronegative groups were variable, suggesting no obvious clustering among the isolates. Similar results were obtained in isolates of M. avium. Conclusions: This is the first report on the molecular epidemiology of Mycobacterium spp. isolated from HIV-seropositive patients in Japan. The results indicate that no particular clones of M. tuberculosis or M. avium prevail in HIV-seropositive patients in Japan. Further monitoring of mycobacterial infection associated with HIV infection in Japan should be continued.
UR - http://www.scopus.com/inward/record.url?scp=29444437548&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=29444437548&partnerID=8YFLogxK
U2 - 10.1016/j.jinf.2004.12.015
DO - 10.1016/j.jinf.2004.12.015
M3 - Article
C2 - 16321647
AN - SCOPUS:29444437548
SN - 0163-4453
VL - 51
SP - 364
EP - 374
JO - Journal of Infection
JF - Journal of Infection
IS - 5
ER -
