Sustained activation of MEK1-ERK1/2 pathway in membrane skeleton occurs dependently on cell adhesion in megakaryocytic differentiation

Chisato Mizutani, Yumi Tohyama, Yasuo Miura, Terutoshi Hishita, Toshio Nishihara, Hirohei Yamamura, Satoshi Ichiyama, Takashi Uchiyama, Kaoru Tohyama

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A human megakaryoblastic cell line, CMK, was treated with 12-o-tetradecanoylphorbol-13-acetate (TPA) for differentiation-induction. We examined TPA-induced activation of the MEK1-ERK1/2 pathway in the 100,000g Triton X-insoluble fraction of CMK cells as the membrane skeleton and researched the relation of the MEK1-ERK1/2 activation with integrin expression. We found that this activation was divided into two phases: the first activation occurred transiently in the membrane skeleton fraction of the suspended cell status and diminished after 1 h; and the second sustained activation was maintained by cell adhesion. TPA-treated CMK cells revealed increased expression of integrins αIIb and β3 only when the cell adhesion persisted, regardless of the difference of culture substratum. Sustained activation of the MEK1-ERK1/2 pathway is generated in the membrane skeleton by continuous cell adhesion and seems to be essential to TPA-induced megakaryocytic differentiation of CMK cells.

Original languageEnglish
Pages (from-to)664-671
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume297
Issue number3
DOIs
Publication statusPublished - 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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