TY - JOUR
T1 - Sustained activation of MEK1-ERK1/2 pathway in membrane skeleton occurs dependently on cell adhesion in megakaryocytic differentiation
AU - Mizutani, Chisato
AU - Tohyama, Yumi
AU - Miura, Yasuo
AU - Hishita, Terutoshi
AU - Nishihara, Toshio
AU - Yamamura, Hirohei
AU - Ichiyama, Satoshi
AU - Uchiyama, Takashi
AU - Tohyama, Kaoru
N1 - Funding Information:
This study was supported in part by grants for Intractable Diseases from the Ministry of Health and Welfare of Japan and by Charitable Trust Clinical Pathology Research Foundation of Japan.
PY - 2002
Y1 - 2002
N2 - A human megakaryoblastic cell line, CMK, was treated with 12-o-tetradecanoylphorbol-13-acetate (TPA) for differentiation-induction. We examined TPA-induced activation of the MEK1-ERK1/2 pathway in the 100,000g Triton X-insoluble fraction of CMK cells as the membrane skeleton and researched the relation of the MEK1-ERK1/2 activation with integrin expression. We found that this activation was divided into two phases: the first activation occurred transiently in the membrane skeleton fraction of the suspended cell status and diminished after 1 h; and the second sustained activation was maintained by cell adhesion. TPA-treated CMK cells revealed increased expression of integrins αIIb and β3 only when the cell adhesion persisted, regardless of the difference of culture substratum. Sustained activation of the MEK1-ERK1/2 pathway is generated in the membrane skeleton by continuous cell adhesion and seems to be essential to TPA-induced megakaryocytic differentiation of CMK cells.
AB - A human megakaryoblastic cell line, CMK, was treated with 12-o-tetradecanoylphorbol-13-acetate (TPA) for differentiation-induction. We examined TPA-induced activation of the MEK1-ERK1/2 pathway in the 100,000g Triton X-insoluble fraction of CMK cells as the membrane skeleton and researched the relation of the MEK1-ERK1/2 activation with integrin expression. We found that this activation was divided into two phases: the first activation occurred transiently in the membrane skeleton fraction of the suspended cell status and diminished after 1 h; and the second sustained activation was maintained by cell adhesion. TPA-treated CMK cells revealed increased expression of integrins αIIb and β3 only when the cell adhesion persisted, regardless of the difference of culture substratum. Sustained activation of the MEK1-ERK1/2 pathway is generated in the membrane skeleton by continuous cell adhesion and seems to be essential to TPA-induced megakaryocytic differentiation of CMK cells.
UR - https://www.scopus.com/pages/publications/0036387182
UR - https://www.scopus.com/pages/publications/0036387182#tab=citedBy
U2 - 10.1016/S0006-291X(02)02235-0
DO - 10.1016/S0006-291X(02)02235-0
M3 - Article
C2 - 12270146
AN - SCOPUS:0036387182
SN - 0006-291X
VL - 297
SP - 664
EP - 671
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -