Synthesis of biotinylated sialoside to probe CD22-ligand interactions

Hajjaj H.M. Abdu-Allah, Kozo Watanabe, Koji Hayashizaki, Yuki Iwayama, Hiromu Takematsu, Yasunori Kozutsumi, Takeshi Tsubata, Hideharu Ishida, Makoto Kiso

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Biotin conjugate of the most potent ligand for mouse CD22 was designed and synthesized. The key synthetic step involved the dual capability of Hanessian reaction for debenzylation and simultaneous conversion of the anomeric p-methoxyphenyl group into the corresponding phenylthioglycoside in one step. Competition enzyme-linked immunosorbent assay (ELISA) for testing the binding affinity of synthetic sialosides was developed based on this biotinylated ligand.

Original languageEnglish
Pages (from-to)4488-4491
Number of pages4
JournalTetrahedron Letters
Volume50
Issue number31
DOIs
Publication statusPublished - 05-08-2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

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