Synthetic glycan ligand excludes CD22 from antigen receptor-containing lipid rafts

Jie Yu, Toshihiko Sawada, Takahiro Adachi, Xiaoming Gao, Hiromu Takematsu, Yasunori Kozutsumi, Hideharu Ishida, Makoto Kiso, Takeshi Tsubata

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

CD22/Siglec-2 is a B cell membrane-bound lectin that recognizes glycan ligands containing α2,6-linked sialic acid, and negatively regulates signaling through the B cell antigen receptor (BCR). Previous studies demonstrated that synthetic sialosides that bind to CD22 augment BCR signaling by inhibiting CD22-mediated BCR regulation. Here we demonstrate that, after antigen stimulation, CD22 forms a cap together with BCR, and translocates to lipid rafts. Both co-capping of CD22 with BCR and translocation of CD22 to lipid rafts were markedly blocked by a synthetic α2,6-linked sialic acid, Neu5Gcα2-6GalβSE. These results strongly suggest that synthetic glycan ligand excludes CD22 from BCR-containing lipid rafts. Because CD22-mediated signal regulation requires phosphorylation of CD22 by Lyn that localizes in lipid rafts and is activated by BCR, synthetic glycan ligand regulates localization of CD22 crucial for signal regulation.

Original languageEnglish
Pages (from-to)759-764
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume360
Issue number4
DOIs
Publication statusPublished - 07-09-2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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