Abstract
The advent of methods for exploring T cell clonal diversity in detail by using the reverse transcriptase-polymerase chain reaction (RT-PCR) to amplify the CDR3 segment of the T cell receptor (TCR) Vβ gene represents a powerful tool for analyzing and monitoring T cell clones that may be responsible for graft-versus-host disease (GVHD) or specific immunity. In this report we describe studies of the posttransplant peripheral blood T cell repertoire in two patients receiving marrow grafts from unrelated donors. One patient received an unmodified T cell replete graft and the second patient received a T cell-depleted marrow graft. Both patients were matched with their donors for HLA-A and -B, but differed for a DRB1 minor mismatch. The patient receiving a TCD graft showed a progressive loss of expression of several Vβ genes during the first 6 months, although expression of some Vβ genes appeared transiently following reduction of immune suppression therapy and evidence of acute GVHD. Spectra-typing of CDR3 segments revealed evolution of new clones and clonal deletion during the post-transplant period. This method in conjunction with a functional analysis and monitoring of host-reactive clones would provide a new approach for evaluating the activity of immunosuppressive therapy.
| Original language | English |
|---|---|
| Pages (from-to) | 125-134 |
| Number of pages | 10 |
| Journal | Human Immunology |
| Volume | 48 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 01-01-1996 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
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T cell receptor clonal diversity following allogeneic marrow grafting. / Akatsuka, Yoshiki; Cerveny, Charles; Hansen, John A.
In: Human Immunology, Vol. 48, No. 1-2, 01.01.1996, p. 125-134.Research output: Contribution to journal › Article
TY - JOUR
T1 - T cell receptor clonal diversity following allogeneic marrow grafting
AU - Akatsuka, Yoshiki
AU - Cerveny, Charles
AU - Hansen, John A.
PY - 1996/1/1
Y1 - 1996/1/1
N2 - The advent of methods for exploring T cell clonal diversity in detail by using the reverse transcriptase-polymerase chain reaction (RT-PCR) to amplify the CDR3 segment of the T cell receptor (TCR) Vβ gene represents a powerful tool for analyzing and monitoring T cell clones that may be responsible for graft-versus-host disease (GVHD) or specific immunity. In this report we describe studies of the posttransplant peripheral blood T cell repertoire in two patients receiving marrow grafts from unrelated donors. One patient received an unmodified T cell replete graft and the second patient received a T cell-depleted marrow graft. Both patients were matched with their donors for HLA-A and -B, but differed for a DRB1 minor mismatch. The patient receiving a TCD graft showed a progressive loss of expression of several Vβ genes during the first 6 months, although expression of some Vβ genes appeared transiently following reduction of immune suppression therapy and evidence of acute GVHD. Spectra-typing of CDR3 segments revealed evolution of new clones and clonal deletion during the post-transplant period. This method in conjunction with a functional analysis and monitoring of host-reactive clones would provide a new approach for evaluating the activity of immunosuppressive therapy.
AB - The advent of methods for exploring T cell clonal diversity in detail by using the reverse transcriptase-polymerase chain reaction (RT-PCR) to amplify the CDR3 segment of the T cell receptor (TCR) Vβ gene represents a powerful tool for analyzing and monitoring T cell clones that may be responsible for graft-versus-host disease (GVHD) or specific immunity. In this report we describe studies of the posttransplant peripheral blood T cell repertoire in two patients receiving marrow grafts from unrelated donors. One patient received an unmodified T cell replete graft and the second patient received a T cell-depleted marrow graft. Both patients were matched with their donors for HLA-A and -B, but differed for a DRB1 minor mismatch. The patient receiving a TCD graft showed a progressive loss of expression of several Vβ genes during the first 6 months, although expression of some Vβ genes appeared transiently following reduction of immune suppression therapy and evidence of acute GVHD. Spectra-typing of CDR3 segments revealed evolution of new clones and clonal deletion during the post-transplant period. This method in conjunction with a functional analysis and monitoring of host-reactive clones would provide a new approach for evaluating the activity of immunosuppressive therapy.
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UR - http://www.scopus.com/inward/citedby.url?scp=0030176090&partnerID=8YFLogxK
U2 - 10.1016/0198-8859(96)00082-1
DO - 10.1016/0198-8859(96)00082-1
M3 - Article
C2 - 8824581
AN - SCOPUS:0030176090
VL - 48
SP - 125
EP - 134
JO - Human Immunology
JF - Human Immunology
SN - 0198-8859
IS - 1-2
ER -