Targeted disruption of the bcl-2 gene in mice exacerbates focal ischemic brain injury

Ryuji Hata, Frank Gillardon, Theologos M. Michaelidis, Konstantin Alexander Hossmann

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Neuronal death after brain ischemia is mainly due to necrosis but there is also evidence for involvement of apoptosis. To test the importance of apoptosis, we investigated the effect of targeted disruption of the apoptosis-suppressive gene bcl-2 on the severity of ischemic brain injury. Transient focal ischemia for 1 hour was induced by occlusion of the middle cerebral artery in homozygous (n = 7) and heterozygous (n = 6) bcl-2 knockout mice as well as in their wildtype littermates (n = 5). Bcl-2 ablation did not influence cerebral blood flow but it significantly increased infarct size and neurological deficit score at 1 day after reperfusion in a gene-dose dependent manner. The exacerbation of tissue damage in the absence of Bcl-2 underscores the importance of apoptotic pathways for the manifestation of ischemic injury after transient vascular occlusion.

Original languageEnglish
Pages (from-to)117-124
Number of pages8
JournalMetabolic Brain Disease
Volume14
Issue number2
DOIs
Publication statusPublished - 14-09-1999

Fingerprint

bcl-2 Genes
Brain Injuries
Brain
Genes
Apoptosis
Cerebrovascular Circulation
Middle Cerebral Artery Infarction
Ablation
Brain Ischemia
Knockout Mice
Reperfusion
Blood Vessels
Blood
Necrosis
Ischemia
Tissue
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Hata, Ryuji ; Gillardon, Frank ; Michaelidis, Theologos M. ; Hossmann, Konstantin Alexander. / Targeted disruption of the bcl-2 gene in mice exacerbates focal ischemic brain injury. In: Metabolic Brain Disease. 1999 ; Vol. 14, No. 2. pp. 117-124.
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Targeted disruption of the bcl-2 gene in mice exacerbates focal ischemic brain injury. / Hata, Ryuji; Gillardon, Frank; Michaelidis, Theologos M.; Hossmann, Konstantin Alexander.

In: Metabolic Brain Disease, Vol. 14, No. 2, 14.09.1999, p. 117-124.

Research output: Contribution to journalArticle

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