Taurine-containing uridine modifications in tRNA anticodons are required to decipher non-universal genetic codes in ascidian mitochondria

Takeo Suzuki, Kenjyo Miyauchi, Tsutomu Suzuki, Shin Ichi Yokobori, Naoki Shigi, Akiko Kondow, Nono Takeuchi, Akihiko Yamagishi, Kimitsuna Watanabe

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Variations in the genetic code are found frequently in mitochondrial decoding systems. Four non-universal genetic codes are employed in ascidian mitochondria: AUA for Met, UGA for Trp, and AGA/AGG(AGR) for Gly. To clarify the decoding mechanism for the non-universal genetic codes, we isolated and analyzed mitochondrial tRNAs for Trp, Met, and Gly from an ascidian, Halocynthia roretzi. Mass spectrometric analysis identified 5-taurinomethyluridine (τm 5U) at the anticodon wobble positions of tRNA Met(AUR), tRNA Trp(UGR), and tRNA Gly-(AGR), suggesting that τm 5U plays a critical role in the accurate deciphering of all four non-universal codes by preventing the misreading of pyrimidine-ending near-cognate codons (NNY) in their respective family boxes. Acquisition of the wobble modification appears to be a prerequisite for the genetic code alteration.

Original languageEnglish
Pages (from-to)35494-35498
Number of pages5
JournalJournal of Biological Chemistry
Volume286
Issue number41
DOIs
Publication statusPublished - 14-10-2011

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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