Taurine-containing uridine modifications in tRNA anticodons are required to decipher non-universal genetic codes in ascidian mitochondria

Takeo Suzuki, Kenjyo Miyauchi, Tsutomu Suzuki, Shin Ichi Yokobori, Naoki Shigi, Akiko Kondo, Nono Takeuchi, Akihiko Yamagishi, Kimitsuna Watanabe

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Variations in the genetic code are found frequently in mitochondrial decoding systems. Four non-universal genetic codes are employed in ascidian mitochondria: AUA for Met, UGA for Trp, and AGA/AGG(AGR) for Gly. To clarify the decoding mechanism for the non-universal genetic codes, we isolated and analyzed mitochondrial tRNAs for Trp, Met, and Gly from an ascidian, Halocynthia roretzi. Mass spectrometric analysis identified 5-taurinomethyluridine (τm 5U) at the anticodon wobble positions of tRNA Met(AUR), tRNA Trp(UGR), and tRNA Gly-(AGR), suggesting that τm 5U plays a critical role in the accurate deciphering of all four non-universal codes by preventing the misreading of pyrimidine-ending near-cognate codons (NNY) in their respective family boxes. Acquisition of the wobble modification appears to be a prerequisite for the genetic code alteration.

Original languageEnglish
Pages (from-to)35494-35498
Number of pages5
JournalJournal of Biological Chemistry
Volume286
Issue number41
DOIs
Publication statusPublished - 14-10-2011

Fingerprint

RNA, Transfer, Trp
Genetic Code
Anticodon
Urochordata
Mitochondria
Uridine
Taurine
Transfer RNA
Decoding
RNA, Transfer, Gly
RNA, Transfer, Met
Codon

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Suzuki, Takeo ; Miyauchi, Kenjyo ; Suzuki, Tsutomu ; Yokobori, Shin Ichi ; Shigi, Naoki ; Kondo, Akiko ; Takeuchi, Nono ; Yamagishi, Akihiko ; Watanabe, Kimitsuna. / Taurine-containing uridine modifications in tRNA anticodons are required to decipher non-universal genetic codes in ascidian mitochondria. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 41. pp. 35494-35498.
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Suzuki, T, Miyauchi, K, Suzuki, T, Yokobori, SI, Shigi, N, Kondo, A, Takeuchi, N, Yamagishi, A & Watanabe, K 2011, 'Taurine-containing uridine modifications in tRNA anticodons are required to decipher non-universal genetic codes in ascidian mitochondria', Journal of Biological Chemistry, vol. 286, no. 41, pp. 35494-35498. https://doi.org/10.1074/jbc.M111.279810

Taurine-containing uridine modifications in tRNA anticodons are required to decipher non-universal genetic codes in ascidian mitochondria. / Suzuki, Takeo; Miyauchi, Kenjyo; Suzuki, Tsutomu; Yokobori, Shin Ichi; Shigi, Naoki; Kondo, Akiko; Takeuchi, Nono; Yamagishi, Akihiko; Watanabe, Kimitsuna.

In: Journal of Biological Chemistry, Vol. 286, No. 41, 14.10.2011, p. 35494-35498.

Research output: Contribution to journalArticle

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AU - Suzuki, Takeo

AU - Miyauchi, Kenjyo

AU - Suzuki, Tsutomu

AU - Yokobori, Shin Ichi

AU - Shigi, Naoki

AU - Kondo, Akiko

AU - Takeuchi, Nono

AU - Yamagishi, Akihiko

AU - Watanabe, Kimitsuna

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N2 - Variations in the genetic code are found frequently in mitochondrial decoding systems. Four non-universal genetic codes are employed in ascidian mitochondria: AUA for Met, UGA for Trp, and AGA/AGG(AGR) for Gly. To clarify the decoding mechanism for the non-universal genetic codes, we isolated and analyzed mitochondrial tRNAs for Trp, Met, and Gly from an ascidian, Halocynthia roretzi. Mass spectrometric analysis identified 5-taurinomethyluridine (τm 5U) at the anticodon wobble positions of tRNA Met(AUR), tRNA Trp(UGR), and tRNA Gly-(AGR), suggesting that τm 5U plays a critical role in the accurate deciphering of all four non-universal codes by preventing the misreading of pyrimidine-ending near-cognate codons (NNY) in their respective family boxes. Acquisition of the wobble modification appears to be a prerequisite for the genetic code alteration.

AB - Variations in the genetic code are found frequently in mitochondrial decoding systems. Four non-universal genetic codes are employed in ascidian mitochondria: AUA for Met, UGA for Trp, and AGA/AGG(AGR) for Gly. To clarify the decoding mechanism for the non-universal genetic codes, we isolated and analyzed mitochondrial tRNAs for Trp, Met, and Gly from an ascidian, Halocynthia roretzi. Mass spectrometric analysis identified 5-taurinomethyluridine (τm 5U) at the anticodon wobble positions of tRNA Met(AUR), tRNA Trp(UGR), and tRNA Gly-(AGR), suggesting that τm 5U plays a critical role in the accurate deciphering of all four non-universal codes by preventing the misreading of pyrimidine-ending near-cognate codons (NNY) in their respective family boxes. Acquisition of the wobble modification appears to be a prerequisite for the genetic code alteration.

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