TY - JOUR
T1 - Tbx6, Thylacine1, and E47 synergistically activate bowline expression in Xenopus somitogenesis
AU - Hitachi, Keisuke
AU - Kondow, Akiko
AU - Danno, Hiroki
AU - Inui, Masafumi
AU - Uchiyama, Hideho
AU - Asashima, Makoto
N1 - Funding Information:
We thank Drs. T. Kinoshita, C. Kintner, Y. Saga, and Y. Sasai for their generous gifts of plasmids. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan. H.D. was also supported by the Japan Society for the Promotion of Science for Young Scientists.
PY - 2008/1/15
Y1 - 2008/1/15
N2 - T-box factor, Tbx6, is a prerequisite for somite segmentation in vertebrates. We recently identified a negative regulator of Tbx6, Bowline, which represses the expression of genes involved in somite segmentation by suppressing the transcriptional activity of Tbx6. According to this function, bowline gene expression is restricted to the most anterior presomitic mesoderm where the somite segmentation program terminates, although it remains unclear how bowline expression is activated. To address this, we investigated the cis-regulatory region of bowline. Measuring luciferase activity driven by the bowline promoter, we found that Tbx6, Thylacine1, and E47 synergistically activate bowline expression in vitro. We also found that Tbx6, Thylacine1, and E47 are spatiotemporally sufficient to induce bowline expression in Xenopus somitogenesis. Our findings indicated that besides being a negative regulator of Tbx6, bowline itself is also regulated by Tbx6, suggesting the negative feedback loop of Tbx6-Bowline in the termination step of somite segmentation.
AB - T-box factor, Tbx6, is a prerequisite for somite segmentation in vertebrates. We recently identified a negative regulator of Tbx6, Bowline, which represses the expression of genes involved in somite segmentation by suppressing the transcriptional activity of Tbx6. According to this function, bowline gene expression is restricted to the most anterior presomitic mesoderm where the somite segmentation program terminates, although it remains unclear how bowline expression is activated. To address this, we investigated the cis-regulatory region of bowline. Measuring luciferase activity driven by the bowline promoter, we found that Tbx6, Thylacine1, and E47 synergistically activate bowline expression in vitro. We also found that Tbx6, Thylacine1, and E47 are spatiotemporally sufficient to induce bowline expression in Xenopus somitogenesis. Our findings indicated that besides being a negative regulator of Tbx6, bowline itself is also regulated by Tbx6, suggesting the negative feedback loop of Tbx6-Bowline in the termination step of somite segmentation.
UR - http://www.scopus.com/inward/record.url?scp=37849051890&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=37849051890&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2007.10.015
DO - 10.1016/j.ydbio.2007.10.015
M3 - Article
C2 - 18035347
AN - SCOPUS:37849051890
SN - 0012-1606
VL - 313
SP - 816
EP - 828
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -