TY - JOUR
T1 - TCL1A Single-Nucleotide Polymorphisms and Estrogen-Mediated Toll-Like Receptor-MyD88–Dependent Nuclear Factor-kB Activation
T2 - Single-Nucleotide Polymorphism– and Selective Estrogen Receptor Modulator–Dependent Modification of Inflammation and Immune Response
AU - Ho, Ming Fen
AU - Ingle, James N.
AU - Bongartz, Tim
AU - Kalari, Krishna R.
AU - Goss, Paul E.
AU - Shepherd, Lois E.
AU - Mushiroda, Taisei
AU - Kubo, Michiaki
AU - Wang, Liewei
AU - Weinshilboum, Richard M.
N1 - Funding Information:
This work was supported in part by the National Institutes of Health (NIH) National Institute of General Medical Sciences [U19 GM61388] and NIH [P50 CA11620, RO1 CA196648, RO1 GM28157, and RO1 CA138461], and the Breast Cancer Research Foundation.
PY - 2017/8
Y1 - 2017/8
N2 - In a previous genome-wide association study (GWAS) for musculoskeletal adverse events during aromatase inhibitor therapy for breast cancer, we reported that single nucleotide polymorphisms (SNPs) near the TCL1A gene were associated with this adverse drug reaction. Functional genomic studies showed that TCL1A expression was induced by estradiol, but only in cells with the variant sequence for the top GWAS SNP (rs11849538), a SNP that created a functional estrogen response element. In addition, TCL1A genotype influenced the downstream expression of a series of cytokines and chemokines and had a striking effect on nuclear factor kB (NF-kB) transcriptional activity. Furthermore, this SNP-dependent regulation could be reversed by selective ER modulators (SERMs). The present study was designed to pursue mechanisms underlying TCL1A SNP-mediated, estrogen-dependent NF-kB activation. Functional genomic studies were performed using a panel of 300 lymphoblastoid cell lines for which we had generated genome-wide SNP and gene expression data. It is known that toll-like receptors (TLRs) can regulate NF-kB signaling by a process that requires the adaptor protein MyD88. We found that TLR2, TLR7, TLR9, and TLR10 expression, as well as that of MyD88, could be modulated by TCL1A in a SNP and estrogen-dependent fashion and that these effects were reversed in the presence of SERMs. Furthermore, MyD88 inhibition blocked the TCL1A SNP and estrogen-dependent NF-kB activation, as well as protein-protein interaction between TCL1A and MyD88. These observations greatly expand the range of pathways influenced by TCL1A genotype and raise the possibility that this estrogen- and SNP-dependent regulation might be altered pharmacologically by SERMs.
AB - In a previous genome-wide association study (GWAS) for musculoskeletal adverse events during aromatase inhibitor therapy for breast cancer, we reported that single nucleotide polymorphisms (SNPs) near the TCL1A gene were associated with this adverse drug reaction. Functional genomic studies showed that TCL1A expression was induced by estradiol, but only in cells with the variant sequence for the top GWAS SNP (rs11849538), a SNP that created a functional estrogen response element. In addition, TCL1A genotype influenced the downstream expression of a series of cytokines and chemokines and had a striking effect on nuclear factor kB (NF-kB) transcriptional activity. Furthermore, this SNP-dependent regulation could be reversed by selective ER modulators (SERMs). The present study was designed to pursue mechanisms underlying TCL1A SNP-mediated, estrogen-dependent NF-kB activation. Functional genomic studies were performed using a panel of 300 lymphoblastoid cell lines for which we had generated genome-wide SNP and gene expression data. It is known that toll-like receptors (TLRs) can regulate NF-kB signaling by a process that requires the adaptor protein MyD88. We found that TLR2, TLR7, TLR9, and TLR10 expression, as well as that of MyD88, could be modulated by TCL1A in a SNP and estrogen-dependent fashion and that these effects were reversed in the presence of SERMs. Furthermore, MyD88 inhibition blocked the TCL1A SNP and estrogen-dependent NF-kB activation, as well as protein-protein interaction between TCL1A and MyD88. These observations greatly expand the range of pathways influenced by TCL1A genotype and raise the possibility that this estrogen- and SNP-dependent regulation might be altered pharmacologically by SERMs.
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U2 - 10.1124/mol.117.108340
DO - 10.1124/mol.117.108340
M3 - Article
C2 - 28615284
AN - SCOPUS:85024090029
SN - 0026-895X
VL - 92
SP - 175
EP - 184
JO - Molecular Pharmacology
JF - Molecular Pharmacology
IS - 2
ER -