Tec protein tyrosine kinase inhibits CD25 expression in human T-lymphocyte

Kentaro Susaki, Akira Kitanaka, Hiroaki Dobashi, Yoshitsugu Kubota, Katsuharu Kittaka, Tomohiro Kameda, Genji Yamaoka, Hiroyuki Mano, Keichiro Mihara, Toshihiko Ishida

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The Tec protein tyrosine kinase (PTK) belongs to a group of structurally related nonreceptor PTKs that also includes Btk, Itk, Rlk, and Bmx. Previous studies have suggested that these kinases play important roles in hematopoiesis and in the lymphocyte signaling pathway. Despite evidence suggesting the involvement of Tec in the T-lymphocyte activation pathway via T-cell receptor (TCR) and CD28, Tec's role in T-lymphocytes remains unclear because of the lack of apparent defects in T-lymphocyte function in Tec-deficient mice. In this study, we investigated the role of Tec in human T-lymphocyte using the Jurkat T-lymphoid cell line stably transfected with a cDNA encoding Tec. We found that the expression of wild-type Tec inhibited the expression of CD25 induced by TCR cross-linking. Second, we observed that LFM-A13, a selective inhibitor of Tec family PTK, rescued the suppression of TCR-induced CD25 expression observed in wild-type Tec-expressing Jurkat cells. In addition, expression of kinase-deleted Tec did not alter the expression level of CD25 after TCR ligation. We conclude that Tec PTK mediates signals that negatively regulate CD25 expression induced by TCR cross-linking. This, in turn, implies that this PTK plays a role in the attenuation of IL-2 activity in human T-lymphocytes.

Original languageEnglish
Pages (from-to)135-142
Number of pages8
JournalImmunology Letters
Volume127
Issue number2
DOIs
Publication statusPublished - 04-01-2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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