Telomere length in non-neoplastic gastric mucosa and its relationship to H. pylori infection, degree of gastritis, and NSAID use

Tomomitsu Tahara, Tomoyuki Shibata, Tomohiko Kawamura, Takamitsu Ishizuka, Masaaki Okubo, Mitsuo Nagasaka, Yoshihito Nakagawa, Tomiyasu Arisawa, Naoki Ohmiya, Ichiro Hirata

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Telomere shortening occurs with human aging in many organs and tissues and is accelerated by rapid cell turnover and oxidative injury. We measured average telomere length using quantitative real-time PCR in non-neoplastic gastric mucosa and assessed its relationship to H. pylori-related gastritis, DNA methylation, ulcer disease, and nonsteroidal anti-inflammatory drug (NSAID) usage. Gastric biopsies were obtained from 151 cancer-free subjects including 49 chronic NSAID users and 102 nonusers. Relative telomere length in genomic DNA was measured by real-time PCR. H. pylori infection status, histological severity of gastritis, and serum pepsinogens (PGs) were also investigated. E-cadherin (CDH1) methylation status was determined by methylation-specific PCR (MSP). Average relative telomere length of H. pylori-infected subjects was significantly shortened when compared to H. pylori-negative subjects (p = 0.002) and was closely associated with all histological parameter of gastritis (all p values <0.01) and CDH1 methylation (p = 0.0002). In H. pylori-negative subjects, NSAID users presented significantly shorter telomere length than nonusers (p = 0.028). Shorter telomere length was observed in duodenal and gastric ulcer patients compared with non-ulcer subjects among NSAID users. Telomere shortening is closely associated with severity of H. pylori-induced gastritis and CDH1 methylation status. Also, telomere shortening is accelerated by NSAID usage especially in H. pylori-negative subjects.

Original languageEnglish
Pages (from-to)65-71
Number of pages7
JournalClinical and Experimental Medicine
Volume16
Issue number1
DOIs
Publication statusPublished - 01-02-2016

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Pylorus
Telomere
Gastritis
Gastric Mucosa
Methylation
Anti-Inflammatory Agents
Telomere Shortening
Infection
Pharmaceutical Preparations
Drug Users
Pepsinogens
Real-Time Polymerase Chain Reaction
Biopsy
Cadherins
Aging of materials
DNA Methylation
Stomach Ulcer
Duodenal Ulcer
Mucous Membrane
Tissue

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tahara, Tomomitsu ; Shibata, Tomoyuki ; Kawamura, Tomohiko ; Ishizuka, Takamitsu ; Okubo, Masaaki ; Nagasaka, Mitsuo ; Nakagawa, Yoshihito ; Arisawa, Tomiyasu ; Ohmiya, Naoki ; Hirata, Ichiro. / Telomere length in non-neoplastic gastric mucosa and its relationship to H. pylori infection, degree of gastritis, and NSAID use. In: Clinical and Experimental Medicine. 2016 ; Vol. 16, No. 1. pp. 65-71.
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Telomere length in non-neoplastic gastric mucosa and its relationship to H. pylori infection, degree of gastritis, and NSAID use. / Tahara, Tomomitsu; Shibata, Tomoyuki; Kawamura, Tomohiko; Ishizuka, Takamitsu; Okubo, Masaaki; Nagasaka, Mitsuo; Nakagawa, Yoshihito; Arisawa, Tomiyasu; Ohmiya, Naoki; Hirata, Ichiro.

In: Clinical and Experimental Medicine, Vol. 16, No. 1, 01.02.2016, p. 65-71.

Research output: Contribution to journalArticle

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